Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-inflammatory Effects of Amelanchier asiatica Fruits Ethanol Extract

채진목 에탄올 추출물의 항염증 효과 검증

  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Yoo, Dan-Hee (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Joo, Da Hye (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Kim, So-Ra (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Jo, Hui-Seon (Gyeonggi-do Forest Environment Research Institute) ;
  • Joo, Sung-Hyun (Department of forestry, Kyungpook National University) ;
  • Chae, Jung-Woo (Gyeonggi-do Forest Environment Research Institute)
  • 이진영 (호서대학교 한방화장품과학과) ;
  • 유단희 (호서대학교 한방화장품과학과) ;
  • 주다혜 (호서대학교 한방화장품과학과) ;
  • 김소라 (호서대학교 한방화장품과학과) ;
  • 조희선 ((재)경기도산림환경연구소) ;
  • 주성현 (경북대학교 임학과) ;
  • 채정우 ((재)경기도산림환경연구소)
  • Received : 2016.10.27
  • Accepted : 2017.03.24
  • Published : 2017.03.31
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Abstract

This study investigated the anti-inflammatory activities and cell viability of Amelanchier asiatica (A. asiatica) 70% ethanol extracts against RAW 264.7 cells induced by lipopolysaccharide (LPS). Cell toxicity test on macrophage cells (RAW 264.7) was performed by 3-[4,5-dimethyl-thiazol-2-yl]-2, 5-diphenyl-tetrazoliumbromide (MTT) assay and results showed 96% cell viability at $1,000{\mu}g/mL$ concentration. Anti-inflammatory activity was examined via the inhibitory tests on the production of LPS induced NO in RAW 264.7 cells by Griess assay. The result showed that the extract inhibited NO production in concentration dependent manner. The iNOS and COX-2 protein expression inhibitory effects were confirmed by western blot and by reverse transcription-polymerase chain reaction (RT-PCR). From the former they were decreased by 84.3%, 56.2% at $500{\mu}g/mL$ concentration, respectively, and from the latter decreased by 89.8%, 84.9% at $500{\mu}g/mL$, respectively. In conclusion, this study showed the anti-inflammatory effects of A. asiatica extracts. Thus, this could be applied to an anti-inflammatory agent.

본 연구에서는 채진목의 항염증 효과를 알아보기 위하여 LPS로 염증을 유도한 RAW 264.7 세포에 대한 채진목 70% 에탄올 추출물의 효과를 살펴보았다. 채진목 70% 에탄올 추출물의 대식세포에서의 세포 독성 측정을 MTT assay를 수행하였다. 세포 독성을 측정한 결과, $1,000{\mu}g/mL$의 농도에서 96%의 세포 생존율을 나타내었다. 항염증 활성을 효과적으로 검증하기 위해, LPS로 유도된 대식세포 내 NO 생산을 억제하는 효과를 Griess의 방법으로 조사하였다. 그 결과, 채진목 70% 에탄올 추출물에서 NO의 생성이 농도 의존적으로 저해되었음을 확인하였다. 채진목 70% 에탄올 추출물을 western blot을 이용하여 단백질 발현을 측정한 결과 처리한 세포군에서 농도가 증가함에 따라 iNOS와 COX-2의 단백질 발현양이 감소하여 최고 농도인 $500{\mu}g/mL$에서 각각 84.3%, 56.2%의 발현 억제를 보여주었다. Reverse transcription-polymerase chain reaction (RT-PCR)을 통하여 mRNA 발현양을 측정한 결과 iNOS와 COX-2의 mRNA 발현양이 감소하여 $500{\mu}g/mL$ 농도에서 89.8%, 84.9%로 나타내었다. 이러한 결과로 보아 채진목 70% 에탄올 추출물의 항염증에 관한 그 효능을 확인할 수 있었고, 따라서 채진목 70% 에탄올 추출물이 천연 항염증 소재로써 가능성이 있다고 판단된다.

Keywords

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