Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Effect of Cationization Agent Concentration on Glycan Detection Using MALDI TOF-MS

  • Kim, Inyoung (Department of Chemistry, Chungnam National University) ;
  • Shin, Dongwon (Department of Chemistry, Chungnam National University) ;
  • Paek, Jihyun (Department of Chemistry, Chungnam National University) ;
  • Kim, Jeongkwon (Department of Chemistry, Chungnam National University)
  • Received : 2017.02.22
  • Accepted : 2017.03.13
  • Published : 2017.03.30
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Abstract

The effect of cationization agent concentration on glycan detection via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was investigated using $Na^+$ ions in the form of NaCl as the cationization agent. NaCl solution concentrations ranging from 1 mM to 1 M were investigated. Glycans from ovalbumin were mixed with the cationization agent solution and the 2,5-dihydroxybenzoic acid (2,5-DHB) matrix solution in a volume ratio of 1:1:1. The resulting mixture was loaded onto the MALDI plate. Two MALDI-TOF MS instruments (Voyager DE-STR MALDI-TOF MS and Tinkerbell RT MALDI-TOF MS) were used for detection of glycans. The best detection, in terms of the number of identified glycans, the peak intensity, and the signal-to-noise (S/N) ratio, was obtained with NaCl concentrations of 0.01-0.1 M for both MALDI-TOF MS instruments.

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