Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of explant parts and medium components on in vitro regeneration in Osmunda cinnamomea var. forkiensis

꿩고비(Osmunda cinnamomea var. forkiensis) 기내 포자체 재생에 영향을 미치는 배양부위와 배지구성물질

  • Kwon, Hyuk Joon (National Institute of Biological Resources) ;
  • Shin, So Lim (National Institute of Biological Resources) ;
  • Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Kim, Soo-Young (National Institute of Biological Resources)
  • 권혁준 (국립생물자원관) ;
  • 신소림 (국립생물자원관) ;
  • 이철희 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 김수영 (국립생물자원관)
  • Received : 2017.06.07
  • Accepted : 2017.10.23
  • Published : 2017.12.31
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Abstract

This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and $NaH_2PO_4$ concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to $50mg{\cdot}L^{-1}\;NaH_2PO_4$ in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, $50mg{\cdot}L^{-1}\;NaH_2PO_4$, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.

본 연구는 꿩고비 포자체의 기내증식을 위한 배양재료 및 배지 구성물질을 구명하기 위해 수행되었다. 포자체의 유묘를 부위별로 나누어 활성탄 0.1%를 첨가한 1/2MS 배지에 다져서 배양한 결과, 근경에서 포자체 재생이 가장 우수하였다. 곱게 다진 근경을 농도를 달리한 MS 배지에 배양한 결과, 1/8MS 배지에서 포자체 형성이 가장 왕성하였으며, 2MS 배지에서는 배양한 절편이 모두 괴사하였다. 상기의 연구를 토대로 1/8MS 배지에 sucrose와 $NaH_2PO_4$의 농도를 조절하여 배양한 결과 sucrose 2%, $NaH_2PO_4\;50mg{\cdot}L^{-1}$에서 포자체 재생이 가장 왕성하였다. 이상의 연구결과, 활성탄 0.1%를 포함한 변형 1/8MS 배지(sucrose 2%, $NaH_2PO_4\;50mg{\cdot}L^{-1}$, agar 0.8%, pH 5.8)에서 포자체 재생이 가장 왕성하였다.

Keywords

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