The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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A study of the Mori Radicis Cortex pre-treatment on transient ischemic brain injury in mice

상백피(桑白皮) 메탄올 추출물 전처치가 일과성 허혈에 의한 생쥐의 뇌 손상에 미치는 영향

  • Chung, Byung-Woo (School of Korean Medicine, Pusan National University) ;
  • Lim, Jae-Yu (School of Korean Medicine, Pusan National University) ;
  • Lee, Se-Eun (School of Korean Medicine, Pusan National University) ;
  • Lee, Byoungho (Hongjae Korean Medicine Clinic) ;
  • Lim, Sehyun (School of Public Health, Far East University) ;
  • Lim, Chiyeon (College of Medicine, Dongguk University) ;
  • Cho, Suin (School of Korean Medicine, Pusan National University)
  • 정병우 (부산대학교 한의학전문대학원) ;
  • 임재유 (부산대학교 한의학전문대학원) ;
  • 이세은 (부산대학교 한의학전문대학원) ;
  • 이병호 (홍재한의원) ;
  • 임세현 (극동대학교 의료보건과학대학) ;
  • 임지연 (동국대학교 의과대학) ;
  • 조수인 (부산대학교 한의학전문대학원)
  • Received : 2016.12.10
  • Accepted : 2017.01.15
  • Published : 2017.01.30
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Abstract

Objectives : Mori Radicis Cortex (MRC), the root epidermis of Morus alba L., has been traditionally used to treat lung-related diseases in Korean Medicine. The common of MRC is Mulberry bark Morus bark, and it's pharmaceutical properties and taste are known as sweet and cold, and it promotes urination and reduce edema by reducing heat from the lungs and soothe asthma. In the present study, anti-apoptotic mechanism of MRC in middle cerebral artery occlusion (MCAO) model in mice. Methods : Two-hundred grams of MRC was extracted with methanol at room temperature for 5 days, and this was repeated one time. After filtration, the methanol was removed using vacuum evaporator, then stored at $-20^{\circ}C$ until use. C57BL/6 male mice were housed in an environment with controlled humidity, temperature, and light cycle. In order to determine beneficial effects of MRC on ischemia induced brain damage, infarct volume, neurological deficit scores, activities of several apoptosis-related proteins such as caspase-8, -9, Bcl-xL in MCAO-induced brains of mice were analyzed. Mice in MRC-treated groups were orally administered 30, 100, or 300 mg/kg of body weight for three consecutive days before commencing the MCAO procedure. Results : Pre-treatment of MRC significantly reduced infarct volume in MCAO subjected mice applied with 300 mg/kg of MRC methanol extract, and MRC effectively inhibited Bcl-xL reduction and caspase-9 activation caused by MCAO-induced brain damage. Conclusions : MRC showed neuro-protective effects by regulating apoptosis-related protein signals, and it can be a potential candidate for the therapy of ischemia-induced brain damage.

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References

  1. Poisson SN, Glidden D, Johnston SC, Fullerton HJ. Deaths from stroke in US young adults, 1989-2009. Neurology. 2014;83(23):2110-2115. https://doi.org/10.1212/WNL.0000000000001042
  2. Bejot Y, Delpont B, Giroud M. Rising stroke incidence in young adults: More epidemiological evidence, more questions to be answered. J Am Heart Assoc. 2016;5(5) :10.1161/JAHA.116.003661.
  3. Hadadha M, Vakili A, Bandegi AR. Effect of the inhibition of hydrogen sulfide synthesis on ischemic injury and oxidative stress biomarkers in a transient model of focal cerebral ischemia in rats. J Stroke Cerebrovasc Dis. 2015;24(12):2676-2684. https://doi.org/10.1016/j.jstrokecerebrovasdis.2015.07.020
  4. Zhao P, Zhou R, Zhu XY. Matrine attenuates focal cerebral ischemic injury by improving antioxidant activity and inhibiting apoptosis in mice. Int J Mol Med. 2015;36(3):633-644. https://doi.org/10.3892/ijmm.2015.2260
  5. Durukan A, Tatlisumak T. Handbook of clinical neurology stroke, part 1 basic and epidemiological aspects. New York: Elsevier; 2009:1-464.
  6. Kim D. Animal models of stroke. Brain & Neurorehabilitation. 2011;4(1):1-11. https://doi.org/10.12786/bn.2011.4.1.1
  7. Park J. Mori Radicis Cortex. The GOSHIGYE, a monthly law journal. 2010;55(12):85-86.
  8. Ham A, Lee HJ, Hong SS, Lee D, Mar W. Moracenin D from Mori Cortex Radicis protects SH-SY5Y cells against dopamine-induced cell death by regulating nurr1 and alpha-synuclein expression. Phytother Res. 2012;26(4):620-624. https://doi.org/10.1002/ptr.3592
  9. Jung JW, Ko WM, Park JH, Seo KH, Oh EJ, Lee DY, Lee DS, Kim YC, Lim DW, Han D, Baek NI. Isoprenylated flavonoids from the root bark of Morus alba and their hepatoprotective and neuroprotective activities. Arch Pharm Res. 2015;38:2066-2075. https://doi.org/10.1007/s12272-015-0613-8
  10. Lee HJ, Lyu da H, Koo U, Lee SJ, Hong SS, Kim K, Kim KH, Lee D, Mar W. Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (Moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells. Arch Pharm Res. 2011;34(8):1373-1380. https://doi.org/10.1007/s12272-011-0818-4
  11. Lee HJ, Lyu da H, Koo U, Nam KW, Hong SS, Kim KO, Kim KH, Lee D, Mar W. Protection of prenylated flavonoids from Mori Cortex Radicis (Moraceae) against nitric oxide-induced cell death in neuroblastoma SH-SY5Y cells. Arch Pharm Res. 2012;35(1):163-170. https://doi.org/10.1007/s12272-012-0118-7
  12. Kong SY, Park MH, Lee M, Kim JO, Lee HR, Han BW, Svendsen CN, Sung SH, Kim HJ. Kuwanon V inhibits proliferation, promotes cell survival and increases Neurogenesis of neural stem cells. PLOS ONE. 2015;10:e0118188. https://doi.org/10.1371/journal.pone.0118188
  13. Wang CP, Zhang LZ, Li GC, Shi YW, Li JL, Zhang XC, Wang ZW, Ding F, Liang XM. Mulberroside a protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion. J Neurosci Res. 2014;92:944-954. https://doi.org/10.1002/jnr.23374
  14. Kang SY, Woo EL, Park YK. Effect of the 70% ethanol extract of Mori Cortex Radidus on ovalbumin-induced allergic asthma in mice. The Korea Journal of Herbology. 2014;29(4):1-8. https://doi.org/10.6116/KJH.2014.29.4.1
  15. Lee KJ, Kim BK, Gil KJ. Suppressive effects of Morus alba Linne root bark (MRAL) on activation of MC/9 mast cells. The Korea Journal of Herbology. 2013;28(1):33-42. https://doi.org/10.6116/kjh.2013.28.1.33
  16. Cho HK. Mori cortex decreases the acute edematous lung injury induced by skin burn. The Korea Journal of Herbology. 2003;18(3):195-202.
  17. Koizumi JI, Yoshida Y, Nakazawa T, Ooneda G. Experimental studies of ischemic brain edema. I. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke. 1986;8:1-8. https://doi.org/10.3995/jstroke.8.1
  18. Roth S, Liesz A. Stroke research at the crossroads- where are we heading? Swiss Med Wkly. 2016;146 :w14329.
  19. Chamorro A, Dirnagl U, Urra X, Planas AM. Neuroprotection in acute stroke: Targeting excitotoxicity, oxidative and nitrosative stress, and inflammation. Lancet Neurol. 2016;15(8):869-881. https://doi.org/10.1016/S1474-4422(16)00114-9
  20. Alberts MJ, Ovbiagele B. Current strategies for ischemic stroke prevention: Role of multimodal combination therapies. J Neurol. 2007;254(10): 1414-1426. https://doi.org/10.1007/s00415-007-0569-9
  21. Pinto A, Tuttolomondo A, Di Raimondo D, Fernandez P, Licata G. Cerebrovascular risk factors and clinical classification of strokes. Semin Vasc Med. 2004;4(3) :287-303. https://doi.org/10.1055/s-2004-861497
  22. Barlow SJ. Identifying the brain regions associated with acute spasticity in patients diagnosed with an ischemic stroke. Somatosens Mot Res. 2016:1-8.
  23. Dragovich T, Rudin CM, Thompson CB. Signal transduction pathways that regulate cell survival and cell death. Oncogene. 1998;17(25):3207-3213. https://doi.org/10.1038/sj.onc.1202587
  24. Wu Y, Tang L. Bcl-2 family proteins regulate apoptosis and epithelial to mesenchymal transition by calcium signals. Curr Pharm Des. 2016.
  25. McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 2015;7(4):10.1101/cshperspect.a026716.
  26. Sun Y, Xu Y, Geng L. Caspase-3 inhibitor prevents the apoptosis of brain tissue in rats with acute cerebral infarction. Exp Ther Med. 2015;10(1): 133-138. https://doi.org/10.3892/etm.2015.2462
  27. Wen XR, Fu YY, Liu HZ, Wu J, Shao XP, Zhang XB, Tang M, Shi Y, Ma K, Zhang F, Wang YW, Tang H, Han D, Zhang P, Wang SL, Xu Z, Song YJ. Neuroprotection of sevoflurane against ischemia/reperfusion-induced brain injury through inhibiting JNK3/caspase-3 by enhancing akt signaling pathway. Mol Neurobiol. 2016;53(3):1661-1671. https://doi.org/10.1007/s12035-015-9111-8
  28. Yang PS, Lin PY, Chang CC, Yu MC, Yen TL, Lan CC, Jayakumar T, Yang CH, Antrodia camphorata potentiates neuroprotection against cerebral ischemia in rats via downregulation of iNOS/HO-1/bax and activated caspase-3 and inhibition of hydroxyl radical formation. Evid Based Complement Alternat Med. 2015;Article ID 232789.

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