The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Improvement Effect of Sibjotang on Blood Glucose and Renal Dysfunction in Type II Diabetic Mice

제2형 당뇨 마우스에서 십조탕(十棗湯)에 의한 혈당 및 신기능 부전 개선효과

  • Yoon, Jung Joo (Department of Physiology, College of Korean Medicine) ;
  • Lee, Yun Jung (Department of Physiology, College of Korean Medicine) ;
  • Kim, Hye Yoom (Department of Physiology, College of Korean Medicine) ;
  • Ahn, You Mee (Department of Physiology, College of Korean Medicine) ;
  • Jin, Xian Jun (Department of Physiology, College of Korean Medicine) ;
  • Hong, Mi Hyeon (Department of Physiology, College of Korean Medicine) ;
  • Hwang, Jin Seok (Department of Physiology, College of Korean Medicine) ;
  • Lee, Ho Sub (Department of Physiology, College of Korean Medicine) ;
  • Kang, Dae Gill (Department of Physiology, College of Korean Medicine)
  • 윤정주 (원광대학교 한의과대학 생리학교실) ;
  • 이윤정 (원광대학교 한의과대학 생리학교실) ;
  • 김혜윰 (원광대학교 한의과대학 생리학교실) ;
  • 안유미 (원광대학교 한의과대학 생리학교실) ;
  • 김현준 (원광대학교 한의과대학 생리학교실) ;
  • 홍미현 (원광대학교 한의과대학 생리학교실) ;
  • 황진석 (원광대학교 한의과대학 생리학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실) ;
  • 강대길 (원광대학교 한의과대학 생리학교실)
  • Received : 2016.10.27
  • Accepted : 2017.01.15
  • Published : 2017.01.30
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Abstract

Objectives : It is well known that Sibjotang (Shizaotang), traditional herbal medicine formula, regulates the body fluid blood pressure homeostasis. This study is to investigate whether Sibjotang improves diabetic renal dysfunction in type II diabetes mellitus animal model, db/db mice. Methods : The animals model were divided into three groups at the age of 8 weeks; control group (C57BLKS/J-db/m mice), diabetic group [(C57BLKS/J+Lepr)-db/db mice], and Sibjotang group [(C57BLKS/J+Lepr)-db/db mice + Sibjotang 100 mg/kg/day]. During 8 weeks of treatment, blood glucose and urinary albumin excretion were checked in metabolic chamber at 8, 12, and 16 weeks of age, respectively. Results : Body weight and food intake of diabetic group were significantly higher than control group after 8 weeks administration. However, there were not significant different between the diabetic group and Sibjotang group. Urinary albumin excretion was significantly decreased in the Sibjotang group than the diabetic group. In addition, supplementation with Sibjotang significantly lowered levels of blood glucose, insulin, and homeostatic model assessment-insulin resistance (HOMA-IR), suggesting reduced insulin resistance. The ratio of mesangial matrix/glomerular area was markedly larger in diabetic group than control group, whereas Sibjotang significantly reduced this expansion. Moreover, immunohistological study revealed that Sibjotang attenuated the increase of transforming growth $factor(TGF)-{\beta}$ expression in kidney. Conclusion : Sibjotang ameliorates diabetes-associated renal injury through the improvement of the blood glucose and insulin sensitivity, and inhibiting the $TGF-{\beta}1$ expression. Therefore, Sibjotang may be a new therapeutic formula for the treatment of diabetic-associated renal dysfunction.

Keywords

References

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