Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
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Genetic Diversity of Salicornia herbacea according to Habitat Area by ISSR Markers

ISSR 마커를 이용한 서식 면적에 따른 퉁퉁마디의 유전적 다양성

  • Kim, Suk-Kyu (Tidal Flat Research Center-National Institute of Fisheries Science) ;
  • Cho, Yoon Sik (Tidal Flat Research Center-National Institute of Fisheries Science) ;
  • Hur, Young Baek (Tidal Flat Research Center-National Institute of Fisheries Science) ;
  • Song, Jae Hee (Tidal Flat Research Center-National Institute of Fisheries Science) ;
  • Jeong, Hee Do (Tidal Flat Research Center-National Institute of Fisheries Science) ;
  • Chung, Sang Ok (Tidal Flat Research Center-National Institute of Fisheries Science)
  • 김석규 (국립수산과학원 서해수산연구소 갯벌연구센터) ;
  • 조윤식 (국립수산과학원 서해수산연구소 갯벌연구센터) ;
  • 허영백 (국립수산과학원 서해수산연구소 갯벌연구센터) ;
  • 송재희 (국립수산과학원 서해수산연구소 갯벌연구센터) ;
  • 정희도 (국립수산과학원 서해수산연구소 갯벌연구센터) ;
  • 정상옥 (국립수산과학원 서해수산연구소 갯벌연구센터)
  • Received : 2017.07.14
  • Accepted : 2017.11.15
  • Published : 2017.12.29
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Abstract

This study analyzed 96 individuals in 6 populations using ISSR marker to investigate the genetic diversity of Salicornia herbacea populations. The total of 49 PCR amplification bands was observed in 6 ISSR primers, and 30 of them had genetic polymorphisms. The Shannon's information index (I) and gene diversity index (h), which indicate the genetic diversity of the Salicornia herbacea populations, were 0.382 and 0.249, respectively. The genetic diversity according to the population size was lowest with 0.092 (I) and 0.058 (h) in $0.1m{\times}0.1m$ and highest with 0.338 (I) and 0.227 (h) in $25m{\times}25m$, which was suitable for the furtherance of the high population with high genetic diversity. The UPGMA dendrogram based on Nei's genetic distance did not show a significant correlation with the distance between the Salicornia herbacea population. The results indicate that the Salicornia herbacea habiting in the restricted environment should have an area over a certain size to ensure the formation of a population with genetic diversity.

퉁퉁마디 개체군의 서식 면적에 따른 유전적 다양성을 조사하기 위하여 6개 군집 96개체를 대상으로 ISSR marker를 사용하여 분석하였다. 6개 ISSR 프라이머에서 총 49개의 PCR 증폭 밴드가 관찰되었으며 이 중 30개의 밴드가 유전적 다형성을 갖는 밴드로 나타났다. 퉁퉁마디 개체군 전체의 유전적 다양성을 나타내는 지수 I(Shannon's information index)는 0.382로 나타났으며 h(gene diversity)는 0.249으로 나타났다. 군집 크기에 따른 유전적 다양성 지수는 $0.1m{\times}0.1m$에서 0.092(I), 0.058(h)로 가장 낮게 나타났고 $25m{\times}25m$에서 0.338(I), 0.227(h)로 가장 높게 나타나 유전적 다양성이 높은 군집 형성에 적합한 면적이라 할 수 있다. 퉁퉁마디 개체군 간 거리에 따른 유전적 다양성의 상관관계를 UPGMA 방법으로 분석한 결과 퉁퉁마디 개체군 간 거리와는 유의한 상관관계를 보이지 않았다. 본 연구 결과 제한된 환경에서 서식하는 퉁퉁마디는 유전적 다양성을 갖는 군집 형성을 위해 일정한 크기 이상의 면적이 확보되어야 할 것으로 판단된다.

Keywords

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