Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Humic Acid Confers HIGH-AFFINITY K+ TRANSPORTER 1-Mediated Salinity Stress Tolerance in Arabidopsis

  • Khaleda, Laila (Division of Applied Life Science (BK21Plus), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Research Institute of Life Sciences (RILS), Gyeongsang National University) ;
  • Park, Hee Jin (Institute of Glocal Disease Control, Konkuk University) ;
  • Yun, Dae-Jin (Department of Biomedical Science and Engineering, Konkuk University) ;
  • Jeon, Jong-Rok (Department of Agriculture Chemistry and Food Science & Technology, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Kim, Min Gab (College of Pharmacy and Research Institute of Pharmaceutical Science, PMBBRC, Gyeongsang National University) ;
  • Cha, Joon-Yung (Division of Applied Life Science (BK21Plus), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Research Institute of Life Sciences (RILS), Gyeongsang National University) ;
  • Kim, Woe-Yeon (Division of Applied Life Science (BK21Plus), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Research Institute of Life Sciences (RILS), Gyeongsang National University)
  • Received : 2017.09.25
  • Accepted : 2017.11.05
  • Published : 2017.12.31
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Abstract

Excessive salt disrupts intracellular ion homeostasis and inhibits plant growth, which poses a serious threat to global food security. Plants have adapted various strategies to survive in unfavorable saline soil conditions. Here, we show that humic acid (HA) is a good soil amendment that can be used to help overcome salinity stress because it markedly reduces the adverse effects of salinity on Arabidopsis thaliana seedlings. To identify the molecular mechanisms of HA-induced salt stress tolerance in Arabidopsis, we examined possible roles of a sodium influx transporter HIGH-AFFINITY $K^+$ TRANSPORTER 1 (HKT1). Salt-induced root growth inhibition in HKT1 overexpressor transgenic plants (HKT1-OX) was rescued by application of HA, but not in wild-type and other plants. Moreover, salt-induced degradation of HKT1 protein was blocked by HA treatment. In addition, the application of HA to HKT1-OX seedlings led to increased distribution of $Na^+$ in roots up to the elongation zone and caused the reabsorption of $Na^+$ by xylem and parenchyma cells. Both the influx of the secondary messenger calcium and its cytosolic release appear to function in the destabilization of HKT1 protein under salt stress. Taken together, these results suggest that HA could be applied to the field to enhance plant growth and salt stress tolerance via post-transcriptional control of the HKT1 transporter gene under saline conditions.

Keywords

References

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  1. One-Pot Transformation of Technical Lignins into Humic-Like Plant Stimulants through Fenton-Based Advanced Oxidation: Accelerating Natural Fungus-Driven Humification vol.3, pp.7, 2017, https://doi.org/10.1021/acsomega.8b00697
  2. Structure and action mechanism of humic substances for plant stimulations vol.38, pp.3, 2017, https://doi.org/10.5333/kgfs.2018.38.3.175
  3. Can Humic Substances Improve Soil Fertility under Salt Stress and Drought Conditions? vol.48, pp.6, 2017, https://doi.org/10.2134/jeq2019.02.0071
  4. Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant vol.8, pp.4, 2017, https://doi.org/10.3390/ht8040018
  5. Quantitative Structure-Activity Relationship of Humic-Like Biostimulants Derived From Agro-Industrial Byproducts and Energy Crops vol.11, pp.None, 2017, https://doi.org/10.3389/fpls.2020.00581
  6. Synergistic Release of Crop Nutrients and Stimulants from Hydroxyapatite Nanoparticles Functionalized with Humic Substances: Toward a Multifunctional Nanofertilizer vol.5, pp.12, 2017, https://doi.org/10.1021/acsomega.9b04354
  7. Extending thermotolerance to tomato seedlings by inoculation with SA1 isolate of Bacillus cereus and comparison with exogenous humic acid application vol.15, pp.4, 2017, https://doi.org/10.1371/journal.pone.0232228
  8. Structural variation of humic-like substances and its impact on plant stimulation: Implication for structure-function relationship of soil organic matters vol.725, pp.None, 2017, https://doi.org/10.1016/j.scitotenv.2020.138409
  9. Impact of Seed Dressing and Soil Application of Potassium Humate on Cotton Plants Productivity and Fiber Quality vol.9, pp.11, 2017, https://doi.org/10.3390/plants9111444
  10. Plant chemical priming by humic acids vol.7, pp.None, 2020, https://doi.org/10.1186/s40538-020-00178-4
  11. Effects of Microbes from Coal-Related Commercial Humic Substances on Hydroponic Crop Cultivation: A Microbiological View for Agronomical Use of Humic Substances vol.69, pp.2, 2017, https://doi.org/10.1021/acs.jafc.0c05474
  12. Which Traits of Humic Substances Are Investigated to Improve Their Agronomical Value? vol.26, pp.3, 2017, https://doi.org/10.3390/molecules26030760
  13. Transcriptome Changes Reveal the Molecular Mechanisms of Humic Acid-Induced Salt Stress Tolerance in Arabidopsis vol.26, pp.4, 2017, https://doi.org/10.3390/molecules26040782
  14. Acclimation with humic acids enhances maize and tomato tolerance to salinity vol.8, pp.1, 2017, https://doi.org/10.1186/s40538-021-00239-2