Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-inflammatory and Anti-cancer Effect of Stachys affinis Tubers

  • Guo, Hui-Fang (Department of Medical Biotechnology, Kangwon National University) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, Kangwon National University)
  • Received : 2017.10.09
  • Accepted : 2017.12.11
  • Published : 2017.12.31
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Abstract

Stachys affinis tubers are known for its high content of stachyose and eaten as an edible vegetable. In this work, we assessed on the anti-inflammatory and anti-proliferation activity of a various type of extracts derived from S. affinis tubers. The n-hexane and dichloromethane fractions were showed the high cytotoxicity on the cell lines including RAW264.7 macrophages, HEK293 human kidney cell, A549 human lung cancer cell, KB human oral cancer cell, and a PC-3 human prostate cancer cell. N-butanol and water fractions were not exhibited cytotoxicity on the tested cancer cells, limited in anti-inflammatory and anti-cancer activities. Nevertheless, the ethyl acetate fraction showed little harm to RAW264.7 cells but inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) significantly. In addition, it arrests the cell growth in A549, KB, and PC-3 cell while little cytotoxicity on HEK293 cells. Consequently, these results supported that the ethyl acetate fraction of S. affinis tubers could be a potential anti-inflammatory and anti-cancer ingredient.

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References

  1. Arulselvan, P., M.T. Fard, W.S. Tan, S. Gothai, S. Fakurazi, M.E. Norhaizan and S.S. Kumar. 2016. Role of antioxidants and natural products in inflammation. Oxid. Med. Cell. Longev. 2016:1-15.
  2. Belayachi, L., C. Aceves-Luquero, N. Merghoub, S.F. de Mattos, S. Amzazi, P. Villalonga and Y. Bakri. 2017. Induction of cell cycle arrest and apoptosis by Ormenis eriolepis a Morrocan endemic plant in various human cancer cell lines. Afr. J. Tradit. Complement. Altern. Med. 14:356. https://doi.org/10.21010/ajtcam.v14i2.37
  3. Bryan, N.S. and J.R. Lancaster Jr. 2011. Nitric oxide signaling in health and disease. In Bryan, N. and J. Loscalzo (eds.), Nitrite and Nitrate in Human Health and Disease. Nutrition and Health. Humana Press, Totowa, NJ (USA). pp. 169-186.
  4. Dos Santos, M.D., M.C. Almeida, N.P. Lopes and G.E.P. De Souza. 2006. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol. Pharm. Bull. 29:2236-2240. https://doi.org/10.1248/bpb.29.2236
  5. El-Rahman, A.A.E.M.A., S.M.A.E.A. ElShafei, E.V. Ivanova, A.N. Fattakhova, A.V. Pankova, M.A.E.A. El-Shafei, E.M. A.E.F. El-Morsi and F.K. Alimova. 2014. Cytotoxicity of Trichoderma spp. cultural filtrate against human cervical and breast cancer cell lines. Asian Pac. J. Cancer Prev. 15:7229-7234. https://doi.org/10.7314/APJCP.2014.15.17.7229
  6. Feng, K., W. Chen, L. Sun, J. Liu, Y. Zhao, L. Li, Y. Wang and W. Zhang. 2015. Optimization extraction, preliminary characterization and antioxidant activity in vitro of polysaccharides from Stachys sieboldii Miq. tubers. Carbohydr. Polym. 125:45-52. https://doi.org/10.1016/j.carbpol.2015.02.026
  7. Gerlier, D. and N. Thomasset. 1986. Use of MTT colorimetric assay to measure cell activation. J. Immunol. Methods 94: 57-63. https://doi.org/10.1016/0022-1759(86)90215-2
  8. Guo, H.F. and M.H. Wang. 2017. Dichloromethane fraction of Asiasarum heterotropoides induces S phase arrest and apoptosis in KB oral epithelial carcinoma cells. Biomed. Pharmacother. 89:559-564. https://doi.org/10.1016/j.biopha.2017.02.072
  9. Kato, K., S. Yokoi, H. Inagaki and Y. Ueno. 1979. Methyl ${\alpha}$-d-Galactopyranoside from the Tubers of Stachys affinis. Agric. Biol. Chem. 43:187-188.
  10. Kim, S.M., J.H. Park, H.O. Boo, S.G. Song and H.Y. Park. 2017. In vitro Comparision of biological activities of solvent fraction extracts from Orostachys japonicus. Korean. J. Plant Res. 30(2):133-143 (in Korean) https://doi.org/10.7732/kjpr.2017.30.2.133
  11. Ko, Y.J., S.M. Song, W.C. Hyun, S.K. Yang, C.K. Song, D.S. Lee and W.J. Yoon. 2014. Anti-inflammatory effect of Castanopsis cuspidata extracts in murine macrophage RAW 264.7 cells. Korean J. Plant Res. 27(5):436-446 (in Korean).
  12. Moilanen, E. and H. Vapaatalo. 1995. Nitric oxide in inflammation and immune response. Ann. Med. 27:359-367. https://doi.org/10.3109/07853899509002589
  13. Nakanishi, M. and D.W. Rosenberg. 2013. Multifaceted roles of PGE2 in inflammation and cancer. In Semin. Immunopathol. 35(2):123-137. https://doi.org/10.1007/s00281-012-0342-8
  14. Nayak, D., S. Ashe, P.R. Rauta, M. Kumari and B. Nayak. 2016. Bark extract mediated green synthesis of silver nanoparticles: Evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Mater. Sci. Eng. C. 58:44-52. https://doi.org/10.1016/j.msec.2015.08.022
  15. Nishimura, H., H. Sasaki, N. Inagaki, C. Masao, Z. Chen and H. Mitsuhashi. 1991. Nine phenethyl alcohol glycosides from Stachys sieboldii. Phytochemistry 30:965-969. https://doi.org/10.1016/0031-9422(91)85288-B
  16. Pacher, P., J.S. Beckman and L. Liaudet. 2007. Nitric oxide and peroxynitrite in health and disease. Physiol. Rev. 87:315-424. https://doi.org/10.1152/physrev.00029.2006
  17. Park, H.J. and Y.J. Cho. 2014. The effect on Anti-oxidative activity and incerasing extraction yield of Aralia elata cortex by Gamma irradiation. Korean J. Plant Res. 27 (5):421-428 (in Korean). https://doi.org/10.7732/kjpr.2014.27.5.421
  18. Rechenberg, D.K., J.C. Galicia and O.A. Peters. 2016. Biological markers for pulpal inflammation: A systematic review. PLoS One 11:e0167289. https://doi.org/10.1371/journal.pone.0167289
  19. Sastry, K.V.H., R.P. Moudgal, J. Mohan, J.S. Tyagi and G.S. Rao. 2002. Spectrophotometric determination of serum nitrite and nitrate by copper-cadmium alloy. Anal. Biochem. 306: 79-82. https://doi.org/10.1006/abio.2002.5676
  20. Sorensen, M., M. Penn, A. El-Mowafi, T. Storebakken, C. Chunfang, M. Overland and ${\AA}$. Krogdahl. 2011. Effect of stachyose, raffinose and soya-saponins supplementation on nutrient digestibility, digestive enzymes, gut morphology and growth performance in Atlantic salmon (Salmo salar L). Aquaculture 314:145-152. https://doi.org/10.1016/j.aquaculture.2011.02.013
  21. Ueno, Y., T. Ikami, R. Yamauchi and K. Kato. 1980. Purification and some properties of ${\alpha}$-galactosidase from tubers of Stachys affinis. Agric. Biol. Chem. 44:2623-2629.
  22. van Meerloo, J., G.J. Kaspers and J. Cloos. 2011. Cell sensitivity assays: the MTT assay. Cancer cell culture: methods and protocols. I.A. Cree, Editor. 2011, Humana Press: Totowa, NJ (USA). pp. 237-245.
  23. Venditti, A., C. Frezza, D. Celona, A. Bianco, M. Serafini, K. Cianfaglione, D. Fiorini, S. Ferraro, F. Maggi and A.R. Lizzi. 2017. Polar constituents, protection against reactive oxygen species, and nutritional value of Chinese artichoke (Stachys affinis Bunge). Food Chem. 221:473-481. https://doi.org/10.1016/j.foodchem.2016.10.096
  24. Vundac, V., Z. Males, M. Plazibat, P. Golja and B. Cetina-Cizmek. 2005. HPTLC determination of flavonoids and phenolic acids in some Croatian Stachys taxa. J. Planar. Chromatogr-Mod. TLC. 18:269-273. https://doi.org/10.1556/JPC.18.2005.4.3
  25. Wang, M.H., S.H. Jeong, H.F. Guo and J.B. Park. 2016. Anti-inflammatory and cytotoxic effects of methanol, ethanol, and water extracts of Angelicae Dahuricae Radix. J. Oral Sci. 58:125-131. https://doi.org/10.2334/josnusd.58.125
  26. Xu, J., Y. Zhao and H.A. Aisa. 2017. Anti-inflammatory effect of pomegranate flower in lipopolysaccharide (LPS)-stimulated RAW264. 7 macrophages. Pharm. Biol. 55:2095-2101. https://doi.org/10.1080/13880209.2017.1357737
  27. Yamahara, J., T. Kitani, H. Kobayashi and Y. Kawahara. 1990. Studies on Stachys sieboldii MIQ. II. Anti-anoxia action and the active constituents. Yakugaku zasshi-J. Pharmaceutical Society of Japan. 110:932-935. https://doi.org/10.1248/yakushi1947.110.12_932
  28. Yi, M.R., C.H. Kang and H.J. Bu. 2017. Anti-inflammatory and tyrosinase inhibition effects of amaranth (Amarantus spp l.) seed extract. Korean. J. Plant Res. 30(2):144-151 (in Korean). https://doi.org/10.7732/kjpr.2017.30.2.144
  29. Yin, J., G. Yang, S. Wang and Y. Chen. 2006. Purification and determination of stachyose in Chinese artichoke (Stachys sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection. Talanta 70:208-212. https://doi.org/10.1016/j.talanta.2006.03.027

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