Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of a Screening Method and Device for the Detection of Escherichia coli from Agri-Food Production Environments and Fresh Produce

  • Yun, Bohyun (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration) ;
  • An, Hyun-Mi (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration) ;
  • Shim, Won-Bo (Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) ;
  • Kim, Won-Il (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration) ;
  • Hung, Nguyen Bao (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration) ;
  • Han, Sanghyun (Division of Research Policy, Research Policy Bureau, Rural Development Administration) ;
  • Kim, Hyun-Ju (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration) ;
  • Lee, Seungdon (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration) ;
  • Kim, Se-Ri (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration)
  • Received : 2017.09.12
  • Accepted : 2017.10.18
  • Published : 2017.12.28
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Abstract

This study was conducted to develop a screening method using Colilert-18 and a device for the detection of E. coli from agri-food production environments and fresh vegetables. The specificity and sensitivity of Colilert-18 by temperature ($37^{\circ}C$ and $44^{\circ}C$) were evaluated with 38 E. coli and 78 non-E. coli strains. The false-positive rate was 3.8% (3/78) and 0% (0/78) at $37^{\circ}C$ and $44^{\circ}C$, respectively. The detection limit of E. coli at $37^{\circ}C$ at <1.0 log CFU/250 ml was lower than that at $44^{\circ}C$. The efficiency of the developed device, which comprised an incubator equipped with a UV lamp to detect E. coli in the field, was evaluated by measuring the temperature and UV lamp brightness. The difference between the set temperature and actual temperature of the developed device was about $1.0^{\circ}C$. When applying the developed method and device to various samples, including utensils, gloves, irrigation water, seeds, and vegetables, there were no differences in detection rates of E. coli compared with the Korean Food Code method. For sanitary disposal of culture samples after experiments, the sterilization effect of sodium dichloroisocyanurate (NaDCC) tablets was assessed for use as a substitute for an autoclave. The addition of one tablet of NaDCC per 50 ml was sufficient to kill E. coli cultured in Colilert-18. These results show that the developed protocol and device can efficiently detect E. coli from agri-food production environments and vegetables.

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