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Defatting and Sonication Enhances Protein Extraction from Edible Insects

  • Choi, Byoung Deug (Department of Food Science and Technology, Chung-Ang University) ;
  • Wong, Nathan A.K. (Department of Food Science and Technology, Chung-Ang University) ;
  • Auh, Joong-Hyuck (Department of Food Science and Technology, Chung-Ang University)
  • Received : 2017.11.27
  • Accepted : 2017.12.11
  • Published : 2017.12.31

Abstract

Edible insects are attracting growing interest as a sustainable source of protein for addition to processed meat and dairy products. The current study investigated the optimal method for protein extraction from mealworm larvae (Tenebrio molitor), cricket adults (Gryllus bimaculatus), and silkworm pupae (Bombyx mori), for use in further applications. After defatting with n-hexane for up to 48 h, sonication was applied for 1-20 min and the protein yield was measured. All samples showed a total residual fat percentage below 1.36%, and a 35% to 94% improvement in protein yield (%). In conclusion, defatting with n-hexane combined with sonication improves the protein yield from insect samples.

Keywords

References

  1. AOAC. (2000) Official methods of analysis of AOAC, 17th Ed. Association of Official Analytical Chemists. Washington DC, USA.
  2. Arzeni, C., Pérez, O. E., and Pilosof, A. M. R. (2012) Functionality of egg white proteins as affected by high intensity ultrasound. Food Hydrocolloid. 29, 308-316. https://doi.org/10.1016/j.foodhyd.2012.03.009
  3. Chandrapala, J., Zisu, B., Palmer, M., Kentish, S., and Ashokkumar, M. (2011) Effects of ultrasound on the thermal and structural characteristics of proteins in reconstituted whey protein concentrate. Ultrason. Sonochem. 18, 951-957. https://doi.org/10.1016/j.ultsonch.2010.12.016
  4. Finke, M. D. (2002) Complete nutrient composition of commercially raised invertebrates used as food for insectivores. Zoo Biol. 21, 269-285. https://doi.org/10.1002/zoo.10031
  5. Fishman, S. M., Caulfield, L. E., de Onis, M., Blossner, M., Hyder, A. A., Mullany, L., and Black, R. E. (2002) Childhood and maternal undernutrition. In: Comparative quantification of health risks. Ezzati, M., A. D. Lopez, A. Rodgers, and C. J. L. Murray, (eds), World Health Organization, Switzerland, pp. 39-161.
  6. Ghosh, S., Lee, S.-M., Jung, C., and Meyer-Rochow, V. B. (2017) Nutritional composition of five commercial edible insects in South Korea. J. Asia-Pac. Entomol. 20, 686-694. https://doi.org/10.1016/j.aspen.2017.04.003
  7. Hall, F. G., Jones, O. G., O Haire, M. E., and Liceaga, A. M. (2017) Functional properties of tropical banded cricket (Gryllodes sigillatus) protein hydrolysates. Food Chem. 224, 414-422. https://doi.org/10.1016/j.foodchem.2016.11.138
  8. Jambrak, A. R., Lelas, V., Mason, T. J., Krešić, G., and Badanjak, M. (2009) Physical properties of ultrasound treated soy proteins. J. Food Eng. 93, 386-393. https://doi.org/10.1016/j.jfoodeng.2009.02.001
  9. Karki, B., Lamsal, B. P., Jung, S., van Leeuwen, J., Pometto, A. L., Grewell, D., and Khanal, S. K. (2010) Enhancing protein and sugar release from defatted soy flakes using ultrasound technology. J. Food Eng. 96, 270-278. https://doi.org/10.1016/j.jfoodeng.2009.07.023
  10. Kim, H.-W., Setyabrata, D., Lee, Y. J., Jones, O. G., and Kim, Y. H. B. (2016) Pre-treated mealworm larvae and silkworm pupae as a novel protein ingredient in emulsion sausages. Innov. Food Sci. Emerg. 38, 116-123. https://doi.org/10.1016/j.ifset.2016.09.023
  11. L Hocine, L., Boye, J. I., and Yves, A. (2006) Composition and functional properties of soy protein isolates prepared using alternative defatting and extraction procedures. J. Food Sci. 71, C137-C145. https://doi.org/10.1111/j.1365-2621.2006.tb15609.x
  12. Lee, H., Yildiz, G., dos Santos, L. C., Jiang, S., Andrade, J. E., Engeseth, N. J., and Feng, H. (2016) Soy protein nano-aggregates with improved functional properties prepared by sequential pH treatment and ultrasonication. Food Hydrocolloid. 55, 200-209. https://doi.org/10.1016/j.foodhyd.2015.11.022
  13. Longvah, T., Mangthya, K., and Ramulu, P. (2011) Nutrient composition and protein quality evaluation of eri silkworm (Samia ricinii) prepupae and pupae. Food Chem. 128, 400-403. https://doi.org/10.1016/j.foodchem.2011.03.041
  14. Lou, Z., Wang, H., Zhang, M., and Wang, Z. (2010) Improved extraction of oil from chickpea under ultrasound in a dynamic system. J. Food Eng. 98, 13-18. https://doi.org/10.1016/j.jfoodeng.2009.11.015
  15. Melo, V., Garcia, M., Sandoval, H., Jimenez, H. D., and Calvo, C. (2011) Quality proteins from edible indigenous insect food of Latin America and Asia. Emir. J. Food Agr. 23, 283-289.
  16. Omotoso, O. T. (2006) Nutritional quality, functional properties and anti-nutrient compositions of the larva of Cirina forda (Westwood) (Lepidoptera: Saturniidae). J. Zhejiang Univ. Sci. B 7, 51-55. https://doi.org/10.1631/jzus.2006.B0051
  17. Preece, K. E., Hooshyar, N., Krijgsman, A. J., Fryer, P. J., and Zuidam, N. J. (2017) Pilot-scale ultrasound-assisted extraction of protein from soybean processing materials shows it is not recommended for industrial usage. J. Food Eng. 206, 1-12. https://doi.org/10.1016/j.jfoodeng.2017.02.002
  18. Purschke, B., Stegmann, T., Schreiner, M., and Jager, H. (2017) Pilot-scale supercritical $CO_2$ extraction of edible insect oil from Tenebrio molitor L. larvae - Influence of extraction conditions on kinetics, defatting performance and compositional properties. Eur. J. Lipid Sci. Tech. 119, 1600134. https://doi.org/10.1002/ejlt.201600134
  19. Ricochon, G. and Muniglia, L. (2010) Influence of enzymes on the oil extraction processes in aqueous media. OCL. 17, 356-359. https://doi.org/10.1051/ocl.2010.0337
  20. Rumpold, B. A. and Schluter, O. K. (2013) Potential and challenges of insects as an innovative source for food and feed production. Innov. Food Sci. Emerg. 17, 1-11. https://doi.org/10.1016/j.ifset.2012.11.005
  21. Tzompa-Sosa, D. A., Yi, L., van Valenberg, H. J. F., van Boekel, M. A. J. S., and Lakemond, C. M. M. (2014) Insect lipid profile: Aqueous versus organic solvent-based extraction methods. Food Res. Int. 62, 1087-1094. https://doi.org/10.1016/j.foodres.2014.05.052
  22. van Huis, A., Itterbeeck, J. V., Klunder, H., Mertens, E., Halloran, A., Muir, G., and Vantomme, P. (2013) Edible insects: Future prospects for food and feed security, Food and Agriculture Organization of the United Nations.
  23. Vilkhu, K., Mawson, R., Simons, L., and Bates, D. (2008) Applications and opportunities for ultrasound assisted extraction in the food industry - A review. Innov. Food Sci. Emerg. 9, 161-169. https://doi.org/10.1016/j.ifset.2007.04.014
  24. Wang, W., Wang, N., Liu, C., and Jin, J. (2017) Effect of silkworm pupae peptide on the fermentation and quality of yogurt. J. Food Process. Pres. 41, e12893. https://doi.org/10.1111/jfpp.12893
  25. Yi, L., Lakemond, C. M., Sagis, L. M., Eisner-Schadler, V., van Huis, A., and van Boekel, M. A. (2013) Extraction and characterisation of protein fractions from five insect species. Food Chem. 141, 3341-3348. https://doi.org/10.1016/j.foodchem.2013.05.115
  26. Yi, L., Van Boekel, M. A. J. S., Boeren, S., and Lakemond, C. M. M. (2016) Protein identification and in vitro digestion of fractions from Tenebrio molitor. Eur. Food Res. Technol. 242, 1285-1297. https://doi.org/10.1007/s00217-015-2632-6
  27. Zhao, X., Vazquez-Gutierrez, J. L., Johansson, D. P., Landberg, R., and Langton, M. (2016) Yellow mealworm protein for food purposes - Extraction and functional properties. PLoS One 11, e0147791. https://doi.org/10.1371/journal.pone.0147791
  28. Zielinska, E., Baraniak, B., Karas, M., Rybczynska, K., and Jakubczyk, A. (2015) Selected species of edible insects as a source of nutrient composition. Food Res. Int. 77, 460-466. https://doi.org/10.1016/j.foodres.2015.09.008

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