Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Whey Protein Attenuates Angiotensin II-Primed Premature Senescence of Vascular Smooth Muscle Cells through Upregulation of SIRT1

  • Hwang, Jung Seok (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University) ;
  • Han, Sung Gu (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University) ;
  • Lee, Chi-Ho (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University) ;
  • Seo, Han Geuk (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University)
  • Received : 2017.11.02
  • Accepted : 2017.11.28
  • Published : 2017.12.31
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Abstract

Whey protein, a by-product of milk curdling, exhibits diverse biological activities and is used as a dietary supplement. However, its effects on stress-induced vascular aging have not yet been elucidated. In this study, we found that whey protein significantly inhibited the Ang II-primed premature senescence of vascular smooth muscle cells (VSMCs). In addition, we observed a marked dose- and time-dependent increase in SIRT1 promoter activity and mRNA in VSMCs exposed to whey protein, accompanied by elevated SIRT1 protein expression. Ang II-mediated repression of SIRT1 level was dose-dependently reversed in VSMCs treated with whey protein, suggesting that SIRT1 is involved in preventing senescence in response to this treatment. Furthermore, resveratrol, a well-defined activator of SIRT1, potentiated the effects of whey protein on Ang II-primed premature senescence, whereas sirtinol, an inhibitor of SIRT1, exerted the opposite. Taken together, these results indicated that whey protein-mediated upregulation of SIRT1 exerts an anti-senescence effect, and can thus ameliorate Ang II-induced vascular aging as a dietary supplement.

Keywords

References

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