Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Metagenomic Analysis of Airborne Bacteria Community and Diversity in Gyeonggi-do, Korea, during March 2016, Asian Dust Event

2016년 한국 경기도의 3월 황사기간 동안 부유세균 군집과 다양성에 대한 메타지노믹 분석

  • Jang, Jun Hyeong (Department of Life Science, Dongguk University-Seoul) ;
  • Kim, Ji Hye (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Bae, Kyung-seon (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Kim, Jeong Myeong (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Lee, Won seok (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Chung, Hyen-mi (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Park, Sangjung (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Seo, Taegun (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
  • 장준형 (동국대학교) ;
  • 김지혜 (국립환경과학원 환경기반연구부 상하수도연구과) ;
  • 배경선 (국립환경과학원 환경기반연구부 상하수도연구과) ;
  • 김정명 (국립환경과학원 환경기반연구부 상하수도연구과) ;
  • 이원석 (국립환경과학원 환경기반연구부 상하수도연구과) ;
  • 정현미 (국립환경과학원 환경기반연구부 상하수도연구과) ;
  • 박상정 (국립환경과학원 환경기반연구부 상하수도연구과) ;
  • 서태근 (국립환경과학원 환경기반연구부 상하수도연구과)
  • Received : 2017.11.20
  • Accepted : 2017.12.18
  • Published : 2017.12.30
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Abstract

Objective: Bacterial abundance and community compositions have been examined in Asian dust events, clarifying their impacts on public health. This study aims to determine the bacterial community compositions and viable bacteria in Asian dust particles in the Asian dust or non-Asian dust event of March 2016. Methods: The dust samples were collected using the high volume air sampler or high volume cascade impactor, and bacterial 16S rRNA genes were amplified using PCR, followed by pyrosequencing. Bacterial diversity index, richness estimate and community composition in the particles were analyzed from the sequencing data using Mothur software. Results: The results showed that the diversity and richness during Asian dust events were higher than them in non-Asian dust events. The total bacterial community analysis showed that at the phylum Proteobacteria, Actinobacteria and Firmicutes were the most dominant of Asian dust events and non-Asian dust events. In addition, the bacterial colony counts were higher during Asian dust event, comparing with non-Asian dust event. Conclusions: This study showed that bacterial community and richness of Asian dust samples was more complex and higher than non-Asian dust samples in Gyeonggi-do, Korea, which could affect public health and environment. Thus, the continuous monitoring of Asian dust could be an alternative for managing airborne bacteria.

Keywords

References

  1. Atkinson RW, Carey IM, Kent AJ, van Staa TP, Anderson HR, Cook DG. Long-term exposure to outdoor air pollution and incidence of cardiovascular diseases. Epidemiology. 2013; 24(1): 44-53. https://doi.org/10.1097/EDE.0b013e318276ccb8
  2. Cho YS, Lee JT, SON JY, Kim YS. A Meta-Analysis of Air Pollution Relation to Daily Mortality in Seven Major Cities of Korea, 1998-2001. J Environ Health Sci. 2006; 32(4): 304-315.
  3. Cho YS, Son JY, Lee JT. Air Pollution Exposure and Low Birth Weight of Firstborn Fetus - A Birth Cohort Studyin Seoul, 1999-2003. J Environ Health Sci. 2007; 33(4): 227-234.
  4. Pascal M, Corso M, Chanel O, Declercq C, Badaloni C, Cesaroni G, et al. Assessing the public health impacts of urban air pollution in 25 European cities: results of the Aphekom project. Sci Total Environ. 2013; 449: 390-400. https://doi.org/10.1016/j.scitotenv.2013.01.077
  5. Kim SH, Chung YS. On the observation of Sandstorms and Associated Episodes of Airborne Dustfalls in the East Asian Region in 2005. J Korean Earth Sci Soc. 2009; 30(2): 196-209. https://doi.org/10.5467/JKESS.2009.30.2.196
  6. Lee MY, Lee CW, Suh SK. Effect of Air Pollution on Daily Mortakity in Daegu (1993-1997) Korean J Occup Environ Med. 2000; 12(2): 235-248.
  7. Hong JH, Go YG. The Health Effects of PM2.5-Evidence from Korea. Korean Resource Economics Association. 2003; 12(3): 469-487.
  8. WORLD HEALTH ORGANIZATION(WHO). Economic cost of the health impact of air pollution in Europe: Clean air, health and wealth. WHO Regional office for Europe, Copenhagen; 2015.
  9. Kim YM, Kim YJ, Lee SB, Moon KC, Bae GN. Review on the Recent $PM_{2.5}$ Studies in China. J Korean Soc Atmos Environ. 2015; 31(5): 411-429. https://doi.org/10.5572/KOSAE.2015.31.5.411
  10. Park JW, Lim HY, Kyung SY, An CH, Lee SP, Jeong SH. Detection of Pathogenic Viruses in the Atmosphere during Asian Dust Events in Incheon City. Tuberc Respir Dis. 2005; 59(3): 279-285. https://doi.org/10.4046/trd.2005.59.3.279
  11. Bae KW, Kim JH, Kim YS, Park JS, Jee YK, Lee YK. Microbiological Identification and Distribution of Metal Components in Suspended Particulate Matter during Yellow Sand Phenomena at TaeAn Region in 2003. Tuberc Respir Dis. 2005; 58(2): 279-285.
  12. Kang GU. Concentration Characteristics and Health Effect Assessment of Atmospheric Particulate Matters During Asian Dust Storm Episodes. J Environ Health Sci. 2009; 35(1): 21-35.
  13. Kwon HJ. Toxicity and health effects of Asian dust: a literature review. J Korean Med Assoc. 2012; 55(3): 234-242. https://doi.org/10.5124/jkma.2012.55.3.234
  14. Kang JH, Song CH, Ock SM, Park HJ. Particulate Matter and Skin. Korean J Fam Pract. 2014; 4: 116-121.
  15. Lee KK. The Fluruation Patterns of Conjunctivitis Cases Caused by Asian Dust Storm(ADS) : Focused on the ADS Density and the Accuracy of ADS Forecast. Korean management science review. 2013; 30(1): 91-102. https://doi.org/10.7737/KMSR.2013.30.1.091
  16. Goudie AS. Desert dust and human health disorders. Environ Int. 2014; 63: 101-113. https://doi.org/10.1016/j.envint.2013.10.011
  17. Vasileiadis S, Puhlisi E, Arena M, Cappa F, Cocconcelli PS, Trevisan M. Soli bacterial diversity screening using single 16S rRNA gene V regions coupled with multi-million read generation sequencing technologies. PLos One. 2012; 7(8): e42671. https://doi.org/10.1371/journal.pone.0042671
  18. Schloss PD, et al. Introducing mother: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microb 2009; 75(23): 7537-7541. https://doi.org/10.1128/AEM.01541-09
  19. Lee SW, Park SJ, Kim JH, Min BD, Chng HM, Park SJ. Characterization of Culturable Bacteria in the Atmospheric Environment in Incheon, Korea. J Environ Health Sci. 2016; 42(2): 126-132.
  20. Draxier RR, Hess GD. An overview of the HYSPLIT_4 modelling system for trajectories, dispersion and deposition. Aust Meteorol Mag. 1998; 47(4): 295-308.
  21. Draxler RR. The use of global and mesoscale meteorological model data to predict the transport and dispersion of tracer plumes over Washington, D. C. Weather Forecast. 2006; 21(3): 383-94. https://doi.org/10.1175/WAF926.1
  22. Kim SY, Lee SH. The Analysis of the Weather Characteristics by Source Region of the Asian Dust observed in South Korea. J. Geo. Soc. Korea. 2013; 48(2): 167-183.
  23. Cha S, Lee D, Jang JH, Lim S, Yang D, Seo T. Alterations in the airborne bacterial community during Asian dust events occurring between February and March 2015 in South Korea. Sci Rep. 2016; 16(6): 37271.
  24. Cha SH, Srinivasan S, Jang JH, Lee DW, Lim SR, Kim KS, et al. Metagenomic Analysis of Airborne Bacterial Community and Diversity in Seoul, Korea, during December 2014, Asian Dust Event. PLoS One. 2017; 25:12(1) e0170693. https://doi.org/10.1371/journal.pone.0170693
  25. Jeon EM, Kim HJ, Jung K, Kim JH, Kim MY, Kim YP, et al. Impact of Asian dust events on airborne bacterial community assessed by molecular analyses. Atmos Environ. 2011; 45(25):4313-4321. https://doi.org/10.1016/j.atmosenv.2010.11.054
  26. Koh JY, Jang CG, Cha MJ, Park KN, Kim MK, Kim JS. Distribution and Characteristics of Microorganisms Associated with Settled Particles During Asian Dust Events. Kor J Microbiol. 2012; 48(2): 134-140. https://doi.org/10.7845/kjm.2012.48.2.134
  27. Lee SW,Chung HM,Park SJ,Choe B,Kim JH,Lee BR, et al. Identification and Phylogenetic Analysis of Culturable Bacteria in the Bioareosol from Several Environments. Microbiol Biotechnol Lett. 2015; 43(2):142-149. https://doi.org/10.4014/mbl.1503.03008