한국어병학회지 (Journal of fish pathology)

  • 제30권2호
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  • Pages.149-154
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  • 2017
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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넙치 유래 viral hemorrhagic septicemia virus (VHSV, genotype IVa)에 대한 단클론 항체 생산

Production of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa) from olive flounder

  • 정하나 (전남대학교 수산생명의학과) ;
  • 장민석 (전남대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과)
  • Jeong, Ha-Na (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Jang, Min-Seok (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 투고 : 2017.11.27
  • 심사 : 2017.12.13
  • 발행 : 2017.12.30
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초록

Mouse monoclonal antibodies (MAbs) were produced by using viral hemorrhagic septicemia virus (VHSV, genotype IVa) as an immunogen, isolated from diseased olive flounder (Paralichthys olivaceus). Four hybridoma clones secreting MAbs against VHSV were established. The MAbs were recognized the nucleoprotein (MAb 4), phosphoprotein (MAb 1) and matrix protein (MAbs 2 and 3) of VHSV by western blot analysis. Among them, the MAbs 1 and 4 strongly reacted with the VHSV-infected FHM cells, but not normal FHM cells. In enzyme linked immunosorbent assay, the four MAbs reacted with the VHSV, but not different six fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus and nervous necrosis virus). These results indicate that the MAbs are useful for diagnosis of VHSV infection.

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참고문헌

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피인용 문헌

  1. 신경괴사증바이러스(nervous necrosis virus, RGNNV genotype)에 대한 단클론 항체 생산 vol.51, pp.3, 2018, https://doi.org/10.5657/kfas.2018.0328
  2. VHS (viral hemorrhagic septicemia)의 원인병원체인 VHSV (genotype IVa)에 대한 단클론 항체 개발 vol.32, pp.2, 2019, https://doi.org/10.7847/jfp.2019.32.2.059
  3. Marine birnavirus (MABV)에 대한 단클론 항체 생산 vol.33, pp.2, 2020, https://doi.org/10.7847/jfp.2020.33.2.171