Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Associated Bacterial Community Structures with the Growth of the Marine Centric Diatom Cyclotella meneghiniana: Evidence in Culture Stages

해양 원형 규조류 Cyclotella meneghiniana 성장 연관 미생물 군집구조 분석: 배양단계에 따른 증거

  • Choi, Won-Ji (Department of Biotechnology, Sangmyung University) ;
  • Park, Bum Soo (Department of Biotechnology, Sangmyung University) ;
  • Guo, Ruoyu (Department of Biotechnology, Sangmyung University) ;
  • Ki, Jang-Seu (Department of Biotechnology, Sangmyung University)
  • Received : 2017.08.22
  • Accepted : 2017.11.28
  • Published : 2017.12.30
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Abstract

There are a number of pieces of evidences that suggest a link between marine diatoms and microorganisms, but knowledge about related microbial communities is greatly lacking. The present study investigated the microbial community structures related to the growth of the marine diatom Cyclotella meneghiniana. We collected free-living bacteria (FLB) and particle-associated bacteria (PAB) at each growth stage (e.g., lag, exponential, stationary and death) of the diatom, and analyzed their bacterial 16S rDNA using pyrosequencing. Metagenomics analysis showed that community structures of FLB and PAB differed considerably with the progress of growth stages. FLB showed higher diversity than PAB, but variation in the different growth stages of C. meneghiniana was more evident in PAB. The proportion of the genus Hoeflea, belonging to the order Rhizobiales, was dominant in both FLB and PAB, and it gradually increased with the growth of C. meneghiniana. However, Enhydrobacter clade tended to considerably decrease in PAB. In addition, Marinobacter decreased steadily in FLB, but first increased and then decreased in PAB. These results suggest that Hoeflea, Enhydrobacter, and Marinobacter may be closely related to the growth of diatom C. meneghiniana.

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References

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