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Phylogenetic Status of Two Undescribed Zygomycete Species from Korea: Actinomucor elegans and Mucor minutus

  • Nguyen, Thuong T.T. (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Youn Su (Division of Bioresource Sciences, Kangwon National University) ;
  • Voigt, Kerstin (JMRC at Leibniz Institute for Natural Product Research and Infection Biology e.V. HKI and Friedrich Schiller University Jena) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
  • 투고 : 2017.10.25
  • 심사 : 2017.12.20
  • 발행 : 2017.12.01

초록

During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.

키워드

참고문헌

  1. Voigt K. Chytridiomycota. In: Frey W, editor. Syllabus of plant families-A. Engler's syllabus der pflanzenfamilien. Part 1/1: Blue-green algae, Myxomycetes and Myxomycetelike organisms, phytoparasitic Protists, heterotrophic Heterokontobionta and Fungi. Stuttgart: Borntraeger Verlag; 2012. p. 106-29.
  2. Schostakowitsch W. 1898. Actinomucor repens n. gen. n. sp. Ber Deut Bot Ges 1898;16:155-8.
  3. Benjamin CR, Hesseltine CW. The genus Actinomucor. Mycologia 1957;49:240-9. https://doi.org/10.2307/3755632
  4. Jong SC, Yuan GF. Actinomucor taiwanensis sp. nov., for manufacture of fermented soybean food. Mycotaxon 1985;23: 261-4.
  5. Zheng RY, Liu XY. Actinomucor elegans var. meitauzae, the correct name for A. taiwanensis and Mucor meitauzae (Mucorales, Zygomycota). Nova Hedwigia 2005;80:419-31. https://doi.org/10.1127/0029-5035/2005/0080-0419
  6. Khan ZU, Ahmad S, Mokaddas E, Chandy R, Cano J, Guarro J. Actinomucor elegans var. kuwaitiensis isolated from the wound of a diabetic patient. Antonie Van Leeuwenhoek 2008; 94:343-52. https://doi.org/10.1007/s10482-008-9251-1
  7. Tully CC, Romanelli AM, Sutton DA, Wickes BL, Hospenthal DR. Fatal Actinomucor elegans var. kuwaitiensis infection following combat trauma. J Clin Microbiol 2009;47:3394-9. https://doi.org/10.1128/JCM.00797-09
  8. Hesseltine CW. A millennium of fungi, food and fermentation. Mycologia 1965;57:149-97. https://doi.org/10.2307/3756821
  9. Ma X, Zhou X, Yoshimoto T. Purification and properties of a novel glycine amino peptidase from Actinomucor elegans and its potential application. J Appl Microbiol 2004;97:985-91. https://doi.org/10.1111/j.1365-2672.2004.02373.x
  10. Wang S, Li P, Su J, Liang R, Wu XK. Enhanced glucosamine production with Actinomucor elegans based on stimulating factor of methanol. Indian J Microbiol 2014;54:459-65. https://doi.org/10.1007/s12088-014-0485-5
  11. Karimi K, Arzanlou M, Ahari AB, Ghazi MM. Phenotypic and molecular characterization of the causal agent of chafer beetle mortality in the wheat fields of the Kurdistan province, Iran. J Plant Prot Res 2015;55:227-34. https://doi.org/10.1515/jppr-2015-0031
  12. Benny GL, Humber RA, Voigt K. Zygomycetous fungi: phylum Entomophthoromycota and subphyla Kickxellomycotina, Mortierellomycotina, Mucoromycotina, and Zoopagomycotina. In: McLaughlin DJ, Spatafora JW, editors. The Mycota, Vol. VII, part A, Systematics and evolution. New York: Springer- Verlag; 2014. p. 209-50.
  13. Benny GL. Methods used by Dr. R. K. Benjamin, and other mycologists, to isolate Zygomycetes. Aliso 2008;26:37-61. https://doi.org/10.5642/aliso.20082601.08
  14. Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA, Abdel-Wahab MA, Alvarado P, Alves-Silva G, Ammirati JF, Ariyawansa HA, et al. Fungal diversity notes 253-366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 2016;78:1-237. https://doi.org/10.1007/s13225-016-0366-9
  15. Nguyen TT, Duong TT, Lee HB. Characterization of two new records of mucoralean species isolated from gut of soldier fly larva in Korea. Mycobiology 2016;44:310-3. https://doi.org/10.5941/MYCO.2016.44.4.310
  16. Alves MH, Campos-Takaki GM, Porto AL, Milanez AI. Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease. Braz J Microbiol 2002;33: 325-30.
  17. Thompson DP, Eribo BE. Extracellular enzyme production by Rhizopus and Mucor species on solid media. Can J Microbiol 1984;30:126-8. https://doi.org/10.1139/m84-020
  18. Alvarez E, Stchigel AM, Cano J, Sutton DA, Fothergill AW, Chander J, Salas V, Rinaldi MG, Guarro J. Molecular phylogenetic diversity of the emerging mucoralean fungus Apophysomyces: proposal of three new species. Rev Iberoam Micol 2010;27:80-9. https://doi.org/10.1016/j.riam.2010.01.006
  19. Jacobs K, Botha A. Mucor renisporus sp. nov., a new coprophilous species from Southern Africa. Fungal Divers 2008;29:27-35.
  20. O'Donnell K, Lutzoni FM, Ward TJ, Benny GL. Evolutionary relationships among mucoralean fungi (Zygomycota): evidence for family polyphyly on a large scale. Mycologia 2001;93:286-97. https://doi.org/10.2307/3761650
  21. Walther G, Pawlowska J, Alastruey-Izquierdo A, Wrzosek M, Rodriguez-Tudela JL, Dolatabadi S, Chakrabarti A, de Hoog GS. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 2013;30:11-47. https://doi.org/10.3767/003158513X665070
  22. Tibpromma S, Hyde KD, Jeewon R, Maharachchikumbura SS, Liu JK, Bhat DJ, Jones EB, McKenzie EH, Camporesi E, Bulgakov TS, et al. Fungal diversity notes 491-602: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 2017;83:1-261. https://doi.org/10.1007/s13225-017-0378-0
  23. Lee YS, Jung HY, Lee HB, Kim SH, Shin KS, Eom AH, Kim C, Lee SY, Korean Society of Mycology. National list of species of Korea. Ascomycota, Glomeromycota, Zygomycota, Myxomycota, Oomycota. Incheon: National Institute of Biological Resources; 2015.
  24. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego (CA): Academic Press; 1990. p. 315-22.
  25. Lee HB. Molecular phylogenetic status of Korean strain of Podosphaera xanthii, a causal pathogen of powdery mildew on Japanese thistle (Cirsium japonicum) in Korea. J Microbiol 2012;50:1075-80. https://doi.org/10.1007/s12275-012-2618-z
  26. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
  27. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  28. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  29. Schipper MA. On Mucor mucedo, Mucor flavus and related species. Stud Mycol 1975;10:1-33.
  30. Kwon-Chung KJ. Taxonomy of fungi causing mucormycosis and entomophthoramycosis (zygomycosis) and nomenclature of the disease: molecular mycologic perspectives. Clin Infect Dis 2012;54(Suppl 1):S8-15. https://doi.org/10.1093/cid/cir864
  31. Vitale RG, de Hoog GS, Schwarz P, Dannaoui E, Deng S, Machouart M, Voigt K, van de Sande WW, Dolatabadi S, Meis JF, et al. Antifungal susceptibility and phylogeny of opportunistic members of the order Mucorales. J Clin Microbiol 2012;50:66-75. https://doi.org/10.1128/JCM.06133-11
  32. Mahmud A, Lee R, Munfus-McCray D, Kwiatkowski N, Subramanian A, Neofytos D, Carroll K, Zhang SX. Actinomucor elegans as an emerging cause of mucormycosis. J Clin Microbiol 2012;50:1092-5. https://doi.org/10.1128/JCM.05338-11
  33. Li Y, Sadiq FA, Fu L, Zhu H, Zhong M, Sohail M. Identification of angiotensin I-converting enzyme inhibitory peptides derived from enzymatic hydrolysates of razor clam Sinonovacula constricta. Mar Drugs 2016;14:E110. https://doi.org/10.3390/md14060110