• Title/Summary/Keyword: Zygomycete fungi

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Characterization of Two New Records of Zygomycete Species Belonging to Undiscovered Taxa in Korea

  • Nguyen, Thi Thuong Thuong;Lee, Seo Hee;Bae, Sarah;Jeon, Sun Jeong;Mun, Hye Yeon;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.1
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    • pp.29-37
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    • 2016
  • During a biodiversity survey of undiscovered taxa in Korea, two zygomycetous fungal strains were isolated. The first strain, EML-FSDY6-1 was isolated from a soil sample collected at Dokdo Island in the East Sea of Korea in 2013, and the second strain, EML-DG-NH3-1 was isolated from a rat dung sample collected at Chonnam National University garden, Gwangju, Korea in 2014. Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer, 18S and 28S rDNA, actin and translation elongation $factor-1{\alpha}$ genes. EML-FSDY6-1 and EML-DG-NH3-1 isolates were confirmed as zygomycete species, Absidia pseudocylindrospora and Absidia glauca, respectively. Neither species has previously been described in Korea.

Phylogenetic Status of Two Undescribed Zygomycete Species from Korea: Actinomucor elegans and Mucor minutus

  • Nguyen, Thuong T.T.;Jung, Hee-Young;Lee, Youn Su;Voigt, Kerstin;Lee, Hyang Burm
    • Mycobiology
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    • v.45 no.4
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    • pp.344-352
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    • 2017
  • During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.

Isolation and Characterization of Three Unrecorded Zygomycete Fungi in Korea: Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans

  • Nguyen, Thuong T.T.;Choi, Young-Joon;Lee, Hyang Burm
    • Mycobiology
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    • v.45 no.4
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    • pp.318-326
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    • 2017
  • In a survey of undiscovered taxa in Korea, three zygomycete fungal strains-EML-W31, EML-HGD1-1, and EML-RUS1-1-were isolated from freshwater, grasshopper fecal, and soil samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of internal transcribed spacer and 28S rDNA, the isolates of EML-W31, EML-HGD1-1, and EML-RUS1-1 were confirmed to be Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans, respectively. These species have not been previously described in Korea.

Fungus flora of paddy fields in Korea. II. Fungal flora of paddy fields. (한국 논 토양중의 균류에 관한 연구 II. 토양균류상)

  • Kyung Hee MIN;CHUN Kyung Sook;Tadayoshi ITO;Tatsuo YOKOYAMA
    • Korean Journal of Microbiology
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    • v.20 no.1
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    • pp.41-51
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    • 1982
  • The soil microfungal flora of the paddy fields in Korea was investigated at four different seasons. The fungi were isolated by the dilution plate method from soil samples of two selected sites around Seoul. A total of 85 isolates was obtained as pure cultures and 30 species 13 genera were identified and 11 isolates were unidentified. Among these, 6 species of Deuteromycetous fungi, Penicillium spp., were found to be dominant in paddy field soils. Penicillium funiculosum, P. piceum, P. roqueforti and P. verruculosum were described as new to Korea. P. piceum has remarkable characteristics of the typical columnar head similar to a compact spruce-like evergreen tree. P. requeforti has penicilli variable in pattern with compactly branched metulae and appressed or Paecilomyces variotii were also described. In addition, Zygorhynchus moelleri, a remarkably dominant Zygomycete in Korean paddy soils, produces subglobose sporangia with oval columella and dark colored zygospores of about $35\mu\textrm{m}$ in diameter.

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Rice Straw-Decomposing Fungi and Their Cellulolytic and Xylanolytic Enzymes

  • Lee, Sang-Joon;Jang, Yeong-Seon;Lee, Young-Min;Lee, Jae-Jung;Lee, Han-Byul;Kim, Gyu-Hyeok;Kim, Jae-Jin
    • Journal of Microbiology and Biotechnology
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    • v.21 no.12
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    • pp.1322-1329
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    • 2011
  • Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cellulolytic and xylanolytic enzymes were assessed through a two-step process, wherein highly active cellulase- and/or hemicellulase-producing fungi were selected in a first screening step followed by a second step to isolate the best enzyme-producer. Twenty-five fungal species were first screened for the production of total cellulase (TC), endo-${\beta}$-1,4 glucanase (EG), and endo-${\beta}$-1,4 xylanase (XYL) using solid-state fermentation with rice straw as substrate. From this screening, six species, namely, Aspergillus niger KUC5183, A. ochraceus KUC5204, A. versicolor KUC5201, Mucor circinelloides KUC6014, Trichoderma harzianum 1 KUC5182, and an unknown basidiomycete species, KUC8721, were selected. These six species were then incubated in liquid Mandels' media containing cellulose, glucose, rice straw, or xylan as the sole carbon source and the activities of six different enzymes were measured. Enzyme production was highly influenced by media conditions and in some cases significantly increased. Through this screening process, Trichoderma harzianum 1 KUC5182 was selected as the best enzyme producer. Rice straw and xylan were good carbon sources for the screening of cellulolytic and xylanolytic enzymes.