Egg Development and Early Life History of the Korean Southern Spine Loach, Iksookimia hugowolfeldi (Pisces: Cobitidae)

한국고유종 남방종개 Iksookimia hugowolfeldi (미꾸리과)의 난발생 및 초기생활사

  • Ko, Myeong-Hun (Division of EcoScience, Ewha Womans University) ;
  • Bang, In-Chul (Department of Life Sciences and Biotechnology, Soonchunhyang University)
  • 고명훈 (이화여자대학교 에코과학부) ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Received : 2016.09.08
  • Accepted : 2016.09.26
  • Published : 2016.09.30

Abstract

Egg development and early life history of Korean southern spine loach, Iksookimia hugowolfeldi, were observed in the present study. Eggs were obtained after injecting females with 0.5 mL/kg of Ovarprim. Eggs were artificially fertilized using the dry method in the laboratory. Number of spawned eggs were $1,933{\pm}530per$ individual. Mature eggs were slightly adhesive with light yellowish coloring, and they measured $1.35{\pm}0.03mm$ in diameter. Hatching of the embryo occurred 56 h (50%) after fertilization at water temperature $25^{\circ}C$, and newly hatched larvae were averaged of $5.6{\pm}0.18mm$ in total length. At 5 days after hatching, larvae averaged $7.8{\pm}0.31mm$ in total length and their yolk sacs had been completely absorbed. Beginning at 15 days after hatching, the fish entered the juvenile stage and reached $13.2{\pm}0.87mm$ in total length. At 100 days after hatching, the band patterns and external form of juvenile were similar to those of adults, and they averaged $49.2{\pm}4.29mm$ in total length.

한국 고유종 남방종개 Iksookimia hugowolfeldi의 난발생 및 초기생활사를 밝히기 위하여 조사를 실시하였다. 성숙한 개체 복강에 Ovaprim (0.5 mL/kg)을 주사하여 12시간 경과 후 복부 압박법으로 채란하여 건식법으로 인공수정 시켰다. 산란된 난은 평균 $1,933{\pm}530$개로, 약한 점착성을 띤 밝은 노란색의 분리침성난이었으며, 난경은 $1.35{\pm}0.03mm$였다. 수온 $25^{\circ}C$에서 수정 후 56시간(50%)에 부화하였으며, 크기는 전장 $5.6{\pm}0.18mm$였다. 부화 후 5일에는 전장 $7.8{\pm}0.31mm$로 난황을 모두 흡수하여 후기자어기로 넘어갔으며, 부화 후 15일에는 전장 $13.2{\pm}0.87mm$로 지느러미 기조가 모두 정수가 되어 치어기로 이행하였다. 부화 후 100일에는 전장 $49.2{\pm}4.29mm$로 성장하고 외부형태 및 체측반문이 성어와 비교적 유사하였다.

Keywords

References

  1. Balon, E.K. 1985. Early Life Histories of Fishes: New developmental, ecological and evolutionary perspectives. Dr. W. Junk Publisher, Dordrecht, 280pp.
  2. Blexter, J.H.S. 1974. The Early Life History of Fish. Spring-Verlag, Berlin, 765pp.
  3. Choi, E.K. 2003. Biology of the southern spined loach, Iksookimia hugowolfeldi (Pisces, Cobitidae). Master Thesis, Chonbuk National University, 42pp. (in Korean)
  4. Kim, I.S. 1997. Illustarated Encyclopedia of Fauna & Flora of Korea. Vol. 37 Freshwater Fishes. Ministry of Education. Youngi 629pp. (in Korean)
  5. Kim, I.S. 2009. A review of the spined loaches, family Cobitidae (Cypriniformes) in Korea. Korean J. Ichthyol., 21(Suppl.): 7-28.
  6. Kim, I.S. and E.H. Lee. 1995. Studies on early embryonic development of Niwaella multifasciata (Pisces: Cobitidae). Korean J. Limnol., 28: 455-462. (in Korean)
  7. Kim, I.S. and J.Y. Park. 2007. Freshwater Fishes of Korea. Kyohak Publishing, Seoul 465pp. (in Korean)
  8. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated Book of Korean Fishes. Kyohak Publishing, Seoul, 615pp. (in Korean)
  9. Kim, S.Y., I.S. Kim, K.Y. Jahng and M.H. Chang. 2000. Molecular phylogeny of Korean loaches inferred from mitochondrial DNA Cytochrome b Sequences. Korean J. Ichthyol., 12: 223-229.
  10. Kim, Y.U., Y.S. Park and D.S. Kim. 1987. Development of eggs, larvae and juveniles of loach, Misgurnus mizolepis Günther. Bull. Korean Fish. Soc., 20: 16-23. (in Korean)
  11. Ko, M.H. and I.C. Bang. 2014. Spawning character and early life history of the endangered Korean dwarf loach, Kichulchoia brevifasciata (Teleostei: Cobitidae). Korean J. Ichthyol., 26: 89-98. (in Korean)
  12. Ko, M.H., J.Y. Park and I.S. Kim. 2009. Development of eggs and early life history of Iksookimia longicorpa (Pisces: Cobitidae) from Nakdong River of Korea. Korean J. Ichthyol., 21: 15-22. (in Korean)
  13. Ko, M.H., S.Y. Park and I.C. Bang. 2012. Egg development and early life history of Korean spined loach, Iksookimia koreensis (Pisces: Cobitidae). Korean J. Limnol., 45: 93-101. (in Korean)
  14. Ko, M.H., S.Y. Park and I.C. Bang. 2013. Egg development and early life history of the endangered Korean spine loach, Iksookimia pumila (Pisces: Cobitidae). Korean J. Ichthyol., 25: 65-73. (in Korean)
  15. Ko, M.H. and Y.J. Won. 2015. Egg development and early life history of the Korean spine loach, Cobitis tetralineata (Pisces: Cobitidae). Korean J. Ichthyol., 27: 95-103. (in Korean)
  16. Kottelat, M. 2012. Conspectus Cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidea). Raffles Bull. Zool., 26(Suppl.): 1-199.
  17. Kwan, Y.S. 2015. Molecular phylogenetic and population genetic studies of the freshwater fish family Cobitidae (Teleostei: Cypriniformes) in Korea. Doctoral Thesis, Ewha Womans University, 143pp.
  18. Lee, W.O., K.H. Kim, J.M. Baek, Y.J. Kang, H.Z. Jeon and C.H. Kim. 2011. Embryonic development and early life history of the northern loach, Cobitis pacifica (Pisces: Cobitidae). Korean J. Limnol., 44: 1-8. (in Korean)
  19. ME (Ministry of Environment). 2009. Development of Genetic Diversity Analysis, Culture and Ecosystem Restoration Techniques for Endangered Fish, Iksookimia choii. Soonchunhyang University, Asan 537pp. (in Korean)
  20. MLTM (Ministry of Land & Transport and Maritime Affairs). 2010. Culture and Restoration of Endangered Species in the Major Four River Drainages. Soonchunhyang University, Asan 489pp. (in Korean)
  21. MLTM (Ministry of Land & Transport and Maritime Affairs). 2011. Culture and Restoration of Endangered Species in the Major Four River Drainages (II) Soonchunhyang University, Asan, 364pp. (in Korean)
  22. Nalbant, T.T. 1993. Some problems in the systematics of the genus Cobitis and its relative (Pisces: Ostariophysi, Cobitidae). Rev. Roum. Biol. (Biol. Anim.), 38: 101-110.
  23. Okada, Y. 1959-1960. Studies on the fresh water fishes of Japan. J. Fac. Fish. Mie Pref. Univ., 4: 433-568.
  24. Park, C.W. 2016. Ecology of the endemic Korean Southern king spine loach, Iksookimia hugowolfeldi. Master Thesis, Chonbuk National University, 56pp. (in Korean)
  25. Park, J.Y. 1996. A Morphological study on the gonad of species in the family Cobitidae (Pisces: Cypriniformes) from Korea. Doctoral Thesis, Chonbuk National University, pp. 13-42. (in Korean)
  26. Park, J.Y. and I.S. Kim. 2001. Fine structure of oocyte envelopes of three related cobitid species in the genus Iksookimia (Cobitidae). Ichthyol. Res., 48: 71-75. https://doi.org/10.1007/s10228-001-8118-5
  27. Perdices, A., J. Bohlen, V. Slechtova and I. Doadrio. 2016. Molecular evidence for multiple origins of the European spined loaches (Teleostei, Cobitidae). PLoS ONE, 11: e0144628. doi:10.1371. https://doi.org/10.1371/journal.pone.0144628
  28. Saitoh, K. and K. Hosoya. 1988. Cobitis biwae Jordan et Synder. Page 159 in M. Okiyama, ed. An atlas of the early stage fishes in Japan. Tokai University Press. Tokyo, xii+1154pp. (in Japanese)
  29. Song, H.Y., W.J. Kim, W.O. Lee and I.C. Bang. 2008. Morphological development of egg and larvae of Iksookimia choii (Cobitidae). Korean J. Limnol., 41: 104-110. (in Korean)
  30. Song, H.Y., H. Yang, E.M. Cho, H.C. Shin and I.C. Bang. 2009. Morphological development of egg and larvae of Koreocobitis naktongensis (Cobitidae). Korean J. Ichthyol., 21: 247-252. (in Korean)
  31. Shimizu, T., H. Sakai and N. Mizuno. 1998. Embryonic and larval development of a Japanese spinous loach, Cobitis takatsuensis. Ichthyol. Res., 45: 377-384. https://doi.org/10.1007/BF02725190
  32. Slechtova, V., J. Bohlen and A. Perdices. 2008. Molecular phylogeny of the freshwater fish family Cobitidae (Cypriniformes: Teleostei): Delimitation of genera, mitochondrial introgression and evolution of sexual dimorphism. Mol. Phylogenet. Evol., 47: 812-831. https://doi.org/10.1016/j.ympev.2007.12.018
  33. Suzuki, R. 1966. Artificial spawning and early development of the loach, Cobitis delicata Niwa. Bull. Freshwater Fish. Res. Lab., 15: 175-188. (in Japanese)
  34. Uchida, K. 1939. The fishes of Tyosen. Part I. Numatognathi, Eventognathi. Bull. Fish Exp. Sta. Gov. Gener. Tyosen, pp. 428-458. (in Japanese)