International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Development of Quantitative Real-Time PCR Primers for Detection of Streptococcus sobrinus

  • Park, Soon-Nang (Korean Collection for Oral Microbiology, School of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Korean Collection for Oral Microbiology, School of Dentistry, Chosun University)
  • Received : 2016.08.13
  • Accepted : 2016.09.17
  • Published : 2016.09.30
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Abstract

The purpose of this study was to develop Streptococcus sobrinus-specific qPCR primers based on the nucleotide sequence of the RNA polymerase ${\beta}-subunit$ gene (rpoB). The specificity of the primers was determined by conventional polymerase chain reaction (PCR) with 12 strains of S. sobrinus and 50 strains (50 species) of non-S. sobrinus bacteria. The sensitivity of the primers was determined by quantitative real-time PCR (qPCR) with serial dilutions of the purified genomic DNAs (40 ng to 4 fg) of S. sobrinus ATCC $33478^T$. The specificity data showed that the S. sobrinus-specific qPCR primers (RTSsob-F4/RTSsob-R4) detected only the genomic DNAs of S. sobrinus strains with a detection limit of up to 4 fg of S. sobrinus genomic DNA. Our results suggest that the RTSsob-F4/RTSsob-R4 primers are useful in detecting S. sobrinus with high sensitivity and specificity for epidemiological studies of dental caries..

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References

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