Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Influence of Reactive Oxygen Species Produced by Chlorine Dioxide on Induction of Insect Cell Apoptosis

이산화염소의 활성산소 유발에 따른 곤충 세포의 아폽토시스

  • Kim, Minhyun (Department of Bioresource Sciences, Andong National University) ;
  • Kumar, Sunil (Department of Bioresource Sciences, Andong National University) ;
  • Kwon, Hyeok (Department of Biosystems and Biotechnology, Korea University) ;
  • Kim, Wook (Department of Biosystems and Biotechnology, Korea University) ;
  • Kim, Yonggyun (Department of Bioresource Sciences, Andong National University)
  • 김민현 (안동대학교 식물의학과) ;
  • 수닐 쿠마르 (안동대학교 식물의학과) ;
  • 권혁 (고려대학교 바이오시스템공학과) ;
  • 김욱 (고려대학교 바이오시스템공학과) ;
  • 김용균 (안동대학교 식물의학과)
  • Received : 2016.06.27
  • Accepted : 2016.07.19
  • Published : 2016.09.01
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Abstract

Chlorine dioxide has an insecticidal activity via its production of reactive oxygen species (ROS). Its cytotoxic activity has been regarded as a main cause of the insecticidal activity. This study tested a hypothesis that cytotoxicity of chlorine dioxide is resulted from its induction of apoptosis against target cells using ROS. Injection of chlorine dioxide significantly reduced total hemocyte counts of Plodia interpunctella larvae and subsequently killed the larvae. To analyze the cytotoxicity with respect to apoptosis, terminal deoxyribonucleotidyl transferase nick end translation (TUNEL) assay was performed. An insect cell line (Sf9) cells were exposed to different concentrations of chlorine dioxide. TUNEL assay showed that chlorine dioxide induced significant apoptosis of Sf9 cells in a dose-dependent manner. When different concentrations of chlorine dioxide were injected to larvae of P. interpunctella, it showed a dose-dependent induction of apoptosis against hemocytes. However, addition of vitamin E significantly suppressed the apoptosis induction and insecticidal activity of chlorine dioxide in a dose-dependent manner. These results suggest that cytotoxicity of chlorine dioxide is resulted from its induction of apoptosis against insect cells using ROS.

이산화염소는 살충효과를 지니며, 이는 이 물질이 발생시키는 활성산소에 기인된다. 살충효과를 주는 주요 원인으로 이산화염소의 세포독성에 주목하고 있다. 본 연구는 이산화염소가 유발하는 세포독성이 활성산소에 기인한 아폽토시스 유발로 가설을 세우고 이를 검증하였다. 화랑곡나방(Plodia interpunctella) 유충에 이산화염소를 주입한 결과 전체혈구수의 뚜렷한 감소를 보였고, 이후 처리 유충은 사망하였다. 아폽토시스 세포치사과정을 규명하기 위해 TUNEL (terminal deoxynucleotidyl transferase nick end translation) 분석법을 적용하였다. 곤충 세포주의 하나인 Sf9 세포에 서로 다른 이산화염소를 처리하고 TUNEL 분석법으로 관찰한 결과 처리 농도에 비례하여 아폽토시스 비율이 증가하였다. 다음으로 서로 다른 농도의 이산화염소를 화랑곡나방 유충에 주입하고 혈구 세포를 TUNEL 분석법으로 관찰한 결과 이산화염소는 처리 농도에 비례하여 아폽토시스 유발을 나타냈다. 그러나 항산화제인 비타민 E를 이산화염소와 함께 처리하면 비타민 E의 농도에 비례하여 이산화염소의 아폽토시스 유발을 억제하고 이에 따라 살충률도 감소하였다. 이러한 결과는 이산화염소에 기인한 세포독성은 활성산소에 기인한 아폽토시스 유발로 이뤄졌다는 것을 제시하고 있다.

Keywords

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