The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Myometrial relaxation of mice via expression of two pore domain acid sensitive K+ (TASK-2) channels

  • Kyeong, Kyu-Sang (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Hong, Seung Hwa (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Kim, Young Chul (Department of Physiology, Chungbuk National University College of Medicine) ;
  • Choi, Woong (Department of Pharmacology, Chungbuk National University College of Medicine) ;
  • Myung, Sun Chul (Department of Urology, College of Medicine, Chung-Ang University) ;
  • Lee, Moo Yeol (Department of Physiology, College of Medicine, Chung-Ang University) ;
  • You, Ra Young (Department of Physiology, Chungbuk National University College of Medicine) ;
  • Kim, Chan Hyung (Department of Pharmacology, Chungbuk National University College of Medicine) ;
  • Kwon, So Yeon (VHS Medical Center) ;
  • Suzuki, Hikaru (Department of Physiology, Nagoya City University Medical School) ;
  • Park, Yeon Jin (Department of Obstetrics and Gynecology, Cheongju St. Mary's Hospital) ;
  • Jeong, Eun-Hwan (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Kim, Hak Soon (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine) ;
  • Kim, Heon (Department of Preventive Medicine, Chungbuk National University College of Medicine) ;
  • Lim, Seung Woon (Department of Anesthesiology and Pain Medicine, Chungbuk National University College of Medicine) ;
  • Xu, Wen-Xie (Department of Physiology, Shanghai Jiaotong University, School of Medicine) ;
  • Lee, Sang Jin (Department of Medical Education, Chungbuk National University College of Medicine) ;
  • Ji, Il Woon (Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine)
  • Received : 2016.07.04
  • Accepted : 2016.08.08
  • Published : 2016.09.01
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Abstract

Myometrial relaxation of mouse via expression of two-pore domain acid sensitive (TASK) channels was studied. In our previous report, we suggested that two-pore domain acid-sensing $K^+$ channels (TASK-2) might be one of the candidates for the regulation of uterine circular smooth muscles in mice. In this study, we tried to show the mechanisms of relaxation via TASK-2 channels in marine myometrium. Isometric contraction measurements and patch clamp technique were used to verify TASK conductance in murine myometrium. Western blot and immunehistochemical study under confocal microscopy were used to investigate molecular identity of TASK channel. In this study, we showed that TEA and 4-AP insensitive non-inactivating outward $K^+$ current (NIOK) may be responsible for the quiescence of murine pregnant longitudinal myometrium. The characteristics of NIOK coincided with two-pore domain acid-sensing $K^+$ channels (TASK-2). NIOK in the presence of $K^+$ channel blockers was inhibited further by TASK inhibitors such as quinidine, bupivacaine, lidocaine, and extracellular acidosis. Furthermore, oxytocin and estrogen inhibited NIOK in pregnant myometrium. When compared to non-pregnant myometrium, pregnant myometrium showed stronger inhibition of NIOK by quinidine and increased immunohistochemical expression of TASK-2. Finally, TASK-2 inhibitors induced strong myometrial contraction even in the presence of L-methionine, a known inhibitor of stretch-activated channels in the longitudinal myometrium of mouse. Activation of TASK-2 channels seems to play an essential role for relaxing uterus during pregnancy and it might be one of the alternatives for preventing preterm delivery.

Keywords

References

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