The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Nafamostat mesilate promotes endothelium-dependent vasorelaxation via the Akt-eNOS dependent pathway

  • Choi, Sujeong (Department of physiology & BK21Plus CNU Integrative Biomedical Education Initiative, School of Medicine, Chungnam National University) ;
  • Kwon, Hyon-Jo (Department of Neurosurgery, Regional Cerebrovascular Center, Chungnam National University Hospital) ;
  • Song, Hee-Jung (Department of Neurology, Chungnam National University Hospital) ;
  • Choi, Si Wan (Division of Cardiology, Internal Medicine, School of Medicine, Chungnam National University, Chungnam National University Hospital) ;
  • Nagar, Harsha (Department of physiology & BK21Plus CNU Integrative Biomedical Education Initiative, School of Medicine, Chungnam National University) ;
  • Piao, Shuyu (Department of physiology & BK21Plus CNU Integrative Biomedical Education Initiative, School of Medicine, Chungnam National University) ;
  • Jung, Saet-byel (Department of Endocrinology, Chungnam National University Hospital) ;
  • Jeon, Byeong Hwa (Department of physiology & BK21Plus CNU Integrative Biomedical Education Initiative, School of Medicine, Chungnam National University) ;
  • Kim, Dong Woon (Department of Anatomy & BK21Plus CNU Integrative Biomedical Education Initiative, School of Medicine, Chungnam National University) ;
  • Kim, Cuk-Seong (Department of physiology & BK21Plus CNU Integrative Biomedical Education Initiative, School of Medicine, Chungnam National University)
  • Received : 2016.06.27
  • Accepted : 2016.07.11
  • Published : 2016.09.01
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Abstract

Nafamostat mesilate (NM), a synthetic serine protease inhibitor, has anticoagulant and anti-inflammatory properties. The intracellular mediator and external anti-inflammatory external signal in the vascular wall have been reported to protect endothelial cells, in part due to nitric oxide (NO) production. This study was designed to examine whether NM exhibit endothelium dependent vascular relaxation through Akt/endothelial nitric oxide synthase (eNOS) activation and generation of NO. NM enhanced Akt/eNOS phosphorylation and NO production in a dose- and time-dependent manner in human umbilical vein endothelial cells (HUVECs) and aorta tissues obtained from rats treated with various concentrations of NM. NM concomitantly decreased arginase activity, which could increase the available arginine substrate for NO production. Moreover, we investigated whether NM increased NO bioavailability and decreased aortic relaxation response to an eNOS inhibitor in the aorta. These results suggest that NM increases NO generation via the Akt/eNOS signaling pathway, leading to endothelium-dependent vascular relaxation. Therefore, the vasorelaxing action of NM may contribute to the regulation of cardiovascular function.

Keywords

References

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