Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidant Activity and Antimicrobial Effect for Foodborne Pathogens from Extract and Fractions of Sanguisorba officinalis L.

지유 추출물 및 분획물의 항산화 활성과 식중독 원인균에 대한 항균활성

  • Seo, Go Eun (Biotechnology Industrialization Center, Dongshin University) ;
  • Kim, Sun Min (Deptartment of Oriental Medicine Materials, Dongshin University) ;
  • Pyo, Byoung Sik (Deptartment of Oriental Medicine Materials, Dongshin University) ;
  • Yang, Sun A (Biotechnology Industrialization Center, Dongshin University)
  • 서고은 (동신대학교 생물자원산업화지원센터) ;
  • 김선민 (동신대학교 한약재산업학과) ;
  • 표병식 (동신대학교 한약재산업학과) ;
  • 양선아 (동신대학교 생물자원산업화지원센터)
  • Received : 2016.07.03
  • Accepted : 2016.08.05
  • Published : 2016.08.30
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Abstract

Background: This study aimed to investigate the antioxidat and antimicrobial activities of the methanol extract and its fractions prepared from the roots of Sanguisorba officinalis L. Methods and Results: The antioxidant activities were compared by evaluating the DPPH radical and nitric oxide (NO) scavenging ability. Measurement of DPPH radical scavenging ability showed that the $SC_{50}$ values of the ethyl acetate fraction was $3.85{\mu}g/m{\ell}$. The ethyl acetate fraction exhibited the most effective DPPH radical scavenging ability compared with the other samples. As for the NO scavenging ability, at all tested concentrations, the ethyl acetate fraction showed a higher scavenging activity than that of the extract and other fractions. These results are related to the total phenolic compound and flavonoid contents of the ethyl acetate fraction. Antimicrobial activity against foodborne pathogens was investigated using the disc diffusion assay. The ethyl acetate fraction showed the highest antimicrobial activity against gram-positive Staphylococcus aureus and Bacillus cereus. However, the chloroform fraction had a higher antimicrobial activity against gram-negative Vibrio vulnificus than that of the extract and other fractions. Conclusions: The results show that the ethyl acetate fraction had a higher antioxidant as well as antimicrobial activity, than did the other samples. Therefore, the ethyl acetate fraction has potential application in the food industry.

Keywords

References

  1. Ahn DJ, Kwak YS, Kim MJ, Lee JC, Shin CS and Jeong KT. (2000). Screening of herbal plant extracts showing antimicrobial activity against some food spoilage and pathogenic microorganisms. Korean Journal of Medicinal Crop Science. 8:109-116.
  2. An BJ, Lee JT, Lee SA, Kwak JH, Park JM, Lee JY and Son JH. (2004a). Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis L. Journal of the Korean Society for Applied Biological Chemistry. 47:244-250.
  3. An BJ, Lee SA, Son JH, Kwak JH, Park JM and Lee JY. (2004b). Cytotoxic and antibacterial activities of Sanguisorba officinalis L. Journal of the Korean Society for Applied Biological Chemistry. 47:141-145.
  4. An HJ, Park KJ, Kim SS, Hyun JM, Park JH, Park SM and Yun SH. (2014). Antioxidative activities of new citrus hybrid 'Hamilgam' peel extracts. Korean Journal of Medicinal Crop Science. 22:442-448. https://doi.org/10.7783/KJMCS.2014.22.6.442
  5. Ban JY, Cho SO, Jeon SY, Song KS, Bae KH and Seong YH. (2005). Protective effect of Sanguisorba officinalis L. root on amyloid ${\beta}$ protein(25-35)-induced neuronal cell damage in cultured rat cortical neuron. Korean Journal of Medicinal Crop Science. 13:219-226.
  6. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  7. Bryan LE. (1982). Current problems of resistant bacteria. Cambridge University Press. Cambridge, England. p.161-191.
  8. Choi MY and Rhim TJ. (2013). Antimicrobial effects against food-borne pathogens of Sanguisorbae Officinalis L. ethanol extract. The Korean Journal of Community Living Science. 24:27-36. https://doi.org/10.7856/kjcls.2013.24.1.27
  9. Cirino G, Distrutti E and Wallace JL. (2006). Nitric oxide and inflammation. Inflammation and Allergy-Drug Targets. 5:115-119. https://doi.org/10.2174/187152806776383143
  10. Dewick PM. (2002). Medicinal natural products: A biosynthetic approach. John Wiley and Sons, Chichester, England. p.149-151.
  11. Folin O and Denis W. (1912). On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological Chemistry. 12:239-243.
  12. Goun EA, Petrichenko VM, Solodnikov SU, Suhinina TV, Kline MA, Cunningham G, Nguyen C and Miles H. (2002). Anticancer and antithrombin activity of Russian plants. Journal of Ethnopharmacology. 81:337-342. https://doi.org/10.1016/S0378-8741(02)00116-2
  13. Hwang SY, Kim MH, Kang JS and Kim BS. (2014). Study of immunosuppressive activiy and insulin secretion by treated Sanguisorba officinalis. Journal of Physiology and Pathology in Korean Medicine. 28:499-505.
  14. Jang SJ. (2010). Studies on the antimicrobial effect and antioxidative activity of fermented Sanguisorbae radix extract. Ph.D. Thesis. Joongbu National University. p.1-13.
  15. Jo YH, Ok DI and Lee SC. (2009). Antimicrobial characteristics of different parts of guava against food-borne bacteria. Journal of the Korean Society of Food Science and Nutrition. 38:1773-1778. https://doi.org/10.3746/jkfn.2009.38.12.1773
  16. Kim SH, Kim DK, Eom DO, Kim SY, Kim SH and Shin TY. (2002). Sanguisorba officinalis inhibits immediate type allergic reactions. Natural Product Sciences. 8:177-182.
  17. Kim SR, Won JH and Kim MR. (2011). Antimicrobial activity against food hazardous microorganisms and antimutagenicity against Salmonella serotype Typhimurium TA100 of an ethanol extract from Sanguisorba officinalis L. Korean Journal of Food and Cookery Science. 27:17-26. https://doi.org/10.9724/kfcs.2011.27.4.017
  18. Kim YS, Suh HJ and Park S. (2013). Antioxidant and photoprotective activities of various extracts from the roots of Rumex crispus L. Korean Journal of Food Preservation. 20:684-690. https://doi.org/10.11002/kjfp.2013.20.5.684
  19. Lee JJ, Choi HS, Lee JH, Jung CJ and Lee MY. (2005). The effects of ethylacetate fraction of Sanguisorba officinalis L. on experimentally-induced acute gastritis and peptic ulcers in rats. Journal of the Korean Society of Food Science and Nutrition. 34:1545-1552. https://doi.org/10.3746/jkfn.2005.34.10.1545
  20. Manach C, Williamson G, Morand C, Scalbert A and Rmsy C. (2005). Bioavailability and bioefficacy of polyphenols in humans: I. Review of 97 bioavailability studies 1,2,3. The American Journal of Clinical Nutrition. 81:2305-2425.
  21. Marcocci L, Maguire JJ, Droylefaix MT and Packer L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 201:748-755. https://doi.org/10.1006/bbrc.1994.1764
  22. Moreno MIN, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several region of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  23. Oldfield EC. (2001). Emerging foodborne pathogens: Keeping your patients and your families safe. Reviews in Gastroenterological Disorders. 1:177-186.
  24. Park GH, Lee JT and An BJ. (2012a). Anti-microbial effect on Streptococcus mutans and anti-oxidant effect of the butanol fractions of Sanguisorbae Radix. The Korea Journal of herbology. 27:23-29.
  25. Park HJ, Kang SA, Lee JY and Cho YJ. (2012b). Antioxidant activities of extracts from medicinal plants. Korean Journal of Food Preservation. 19:744-750. https://doi.org/10.11002/kjfp.2012.19.5.744
  26. Peterson J and Dwyer J. (1998). Flavonoids: Dietary occurrence and biochemical activity. Nutrition Research. 18:1995-2018. https://doi.org/10.1016/S0271-5317(98)00169-9
  27. Seo YC, Choi WY, Kim JS, Zou YY, Lee CG, Ahn JH, Shin IS and Lee HY. (2010). Enhancement of antimicrobial activity of nano-encapsulated horseradish aqueous extracts against foodborne pathogens. Korean Journal of Medicinal Crop Science. 18:389-397.
  28. Shin JH, Lee HG, Kang MJ, Lee SJ and Sung NJ. (2010). Antioxidant activity of solvent fraction from black garlic. Journal of the Korean Society of Food Science and Nutrition. 39:933-940. https://doi.org/10.3746/jkfn.2010.39.7.933
  29. Son KJ, Lee SA, Lee GD, Kim YS, Jeon JG and Chang KY. (2004). Effects of crude Sanguisorba officinalis L. extract on the growth and the adherence to hydroxyapatite beads of mutans streptococci. Journal of Korean Academy of Oral Health. 28:97-104.
  30. Song JH and Lee SR. (2015). Anti-oxidant and inhibitory activity on NO production of extract and its fractions from Rosa davurica Pall. leaves. Korean Journal of Medicinal Crop Science. 23:20-26. https://doi.org/10.7783/KJMCS.2015.23.1.20
  31. Song YJ, Park SH, You JY, Cho YS and Oh KH. (2009). Antibacterial activity against food-poisoning causing bacteria and characterization of Lactobacillus plantarum YK-9 isolated from Kimchi. Korean Journal of Biotechnology and Bioengineering. 24:273-278.