Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지
DOI QR코드

DOI QR Code

Chemical Compositions and Insecticidal Activity of Eucalyptus urophylla Essential oil Against Culex quinquefasciatus Mosquito

  • Pujiarti, Rini (Department of Forest Product Technology, Faculty of Forestry, Gadjah Mada University) ;
  • Kasmudjo, Kasmudjo (Department of Forest Product Technology, Faculty of Forestry, Gadjah Mada University)
  • Received : 2015.09.18
  • Accepted : 2016.04.22
  • Published : 2016.07.25
Download PDF
⟨ Previous Next ⟩

Abstract

Eucalyptus oils are widely used as spices, perfume industrial materials, food flavorings, and medicines. Several types of Eucalyptus oils also have insecticidal activity and as carminative. This study investigated the chemical composition, insecticidal (larvicidal and repellent) activity of E. urophylla oil against filarial mosquito Culex quinquefasciatus. E. urophylla oil was obtained from fresh leaves by water-steam distillation with oil yield 1.08%. E. urophylla oil in this study had no color (clear), has odor (typical eucalyptus), with specific gravity 0.941; refractive index 1.465; miscibility in 70% ethanol 1 : 3; and optical rotation (-) $5.83^{\circ}$. The major compounds of the oil were ${\alpha}$-pinene (11.73%), 1,8-cineole (49.86%), ${\beta}$-ocimene (6.25%), ${\gamma}$-terpinene (9.11%), and ${\alpha}$-terpinyl acetate (7.63%). The result showed the excellent insecticide activity against C. quinquefasciatus. The oil provided larvicidal activity with $LC_{50}$: 80.21 ppm and $LC_{90}$: 210.18 ppm, and repellent activity with $IC_{50}$: 0.82% and $IC_{90}$: 4.88%. The present study showed the effectiveness of E. urophylla as natural insecticide against C. quinquefasciatus, the mosquito vector of filariasis.

Keywords

References

  1. Agarwal, R., Lakshmi, T. 2013. Eucalyptus oil in dentistry: A mini review. International Journal of Drug Development & Research 5(4): 58-61.
  2. Alzogaray, R.A., Lucia, A., Zerba, E.N., Masuh, H.M. 2011. Insecticidal activity of essential oils from eleven Eucalyptus spp. and two hybrids: Lethal and sublethal effects of their major components on Blattella germanica. Journal of Economic Entomology 104(2): 595-600. https://doi.org/10.1603/EC10045
  3. Anonymous. 2006. Cajuput oil. Indonesia National Standardization, SNI 06-3954-2006. National Standardization Agency of Indonesia, Indonesia.
  4. Anonymous. 2013. Lymphatic Filariasis: A Handbook of Practical Entomology for National Lymphatic Filariasis Elimination Programmes. World Health Organization. Avenue Appia, Geneva.
  5. Astiani, D.P., Jayuska, A., Arreneuz, S. 2014. Antibacterial activity of Eucalyptus pellita essential oil Escherichia coli and Staphylococcus aureus. Equator Journal Chemical 3(3): 44-48.
  6. Baser, K.C.B., Buchbauer, G. 2010. Handbook of Essential Oil, Science, Technology and Application. CRC Press, London, New York.
  7. Briassoulis, G., Narlioglou, M., Hatzis, T. 2001. Toxic encephalopathy associated with use of DEET insect repellents: a case analysis of its toxicity in children. Human & Experimental Toxicology 20: 8-14. https://doi.org/10.1191/096032701676731093
  8. Cheng, S.S., Huang, G.G., Chen, Y.J., Jane Yu, D., Chen, W.J., Chang, S.T. 2009. Chemical compositions and larvicidal activities of leaf essential oils from two Eucalyptus species. Bioresource Technology 100: 452-456. https://doi.org/10.1016/j.biortech.2008.02.038
  9. Chu, S.S., Jiang, G.H. 2011. Insecticidal activity and composition of essential oil of Ostericum sieboldii (Apiaceae) against Sitophilus zeamais and Tribolium castaneum. Records of Natural Products 5(2): 74-81.
  10. Coffi, K., Soleymane, K., Harisolo, R., Bi Balo, T., Claude, C.J., Pierre, C., Gilles, F., Antoine, A.C. 2012. Monoterpene hydrocarbons, major components of the dried leaves essential oils of five species of the genus Eucalyptus from Cote d'Ivoire. Natural Science 4 (2): 106-111. https://doi.org/10.4236/ns.2012.42015
  11. Elaissi, A., Medini, H., Khouja, M.L., Simmonds, M., Lynene, F., Farhat, F., Chemli, R., Harzallah-Skhiri, F. 2010. Variation in volatile leaf oils of eleven Eucalyptus species harvested from Korbous Arboreta (Tunisia). Chemistry & Biodiversity 7(7): 1841-1854. https://doi.org/10.1002/cbdv.200900381
  12. Elaissi, A., Medini, H., Simmonds, M., Lynene, F., Farhat, F., Chemli, R., Harzallah-Skhiri, F. Khouja, M.L. 2011.Variation in volatile leaf oils of seven Eucalyptus species harvested from Zerniza Arboreta (Tunisia). Chemistry & Biodiversity 8(2): 362-372. https://doi.org/10.1002/cbdv.201000103
  13. Joker, D. 2004. Eucalyptus urophylla S.T. Blake. Seed Leaflet. Forest and Landscape.
  14. Maia, M.F., Moore, S.J. 2011. Plant-based insect repellents: A review of their efficacy, development and testing. Malaria Journal 10: 11-15. https://doi.org/10.1186/1475-2875-10-11
  15. Makhaik, M., Naik, S.N., Tewary, D.K. 2005. Evaluation of anti-mosquito properties of essential oils. Journal of Scientific & Industrial Research 64: 129-133.
  16. Manaf, S. 1988. Preliminary Study on The Insectcide Effect of Seed Extract Annona squamosal Against Martinus demestoides. Food and Nutritions Report, Biological Scinence and Biotechnology. Yogyakarta.
  17. Mohtar, M., Yarmo, M.A., Kadri, A. 1999. The effects of Neriumindicum leaf extract on Aedes aegypti larvae. Journal of Tropical Forest Products 5(1): 87-92.
  18. Monzon, R.B., Alvior, J.P., Luczon, L.L.C., Morales, A.S., Mutuc, F.E.S. 1994. Larvacidal potential of five Philippine plants against Aedes aegypty (Linnaeus) and Culex quinquefasciatus (Say.). Southeast Asian Journal of Tropical Medicine Public Health 25: 755-759.
  19. Nerio, L.S., Olivero-Verbel, J., Stashenko, E. 2010. Repellent activity of essential oils: A review. Bioresource Technology 101: 372-378. https://doi.org/10.1016/j.biortech.2009.07.048
  20. Park, H.M., Kim, J., Chang, K.S., Kim, B.S., Yang, Y.J., Kim, G.H. 2011. Larvacidal activity of Myrtaceae essential oil and their components againt Aedes aegypty, acute toxicity on Daphnia Magna and Aqueous Residue. Journal of Medical Entomology 48 (2): 405-410. https://doi.org/10.1603/ME10108
  21. Pujiarti, R., Ohtani, Y., Ichiura, H. 2011. Physicochemical properties and chemical compositions of Melaleuca leucadendron leaf oils taken from the plantations in Java, Indonesia. Journal of Wood Science 57: 446-451. https://doi.org/10.1007/s10086-011-1183-0
  22. Song, A., Wang, Y., Liu, Y. 2009. Study on the chemical constituents of the essential oil of the leaves of Eucalyptus globulus Labill. Asian Journal of Traditional Medicines 4(4): 134-140.
  23. Ukeh, D.A., Sylvia, B.A., Umoetok. 2011. Repellent effects of five monoterpenoid odours against Tribolium castaneum (Herbst) and Rhyzopertha dominica (F.) in Calabar, Nigeria. Crop Protection 30: 1351-1355. https://doi.org/10.1016/j.cropro.2011.05.016
  24. WHOPES (World Health Organization Pesticides Evaluation Scheme). 2009. Guideline for Efficacy Testing of Mosquito Repellent for Human Skin. Geneva.