Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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H2O2 Pretreatment Modulates Growth and the Antioxidant Defense System of Drought-stressed Zoysiagrass and Kentucky Bluegrass

  • Bae, Eun-Ji (Southern Forest Resource Research Center, National Institute of Forest Science) ;
  • Han, Jeong-Ji (Southern Forest Resource Research Center, National Institute of Forest Science) ;
  • Choi, Su-Min (Southern Forest Resource Research Center, National Institute of Forest Science) ;
  • Lee, Kwang-Soo (Southern Forest Resource Research Center, National Institute of Forest Science) ;
  • Park, Yong-Bae (Southern Forest Resource Research Center, National Institute of Forest Science) ;
  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University)
  • Received : 2016.02.21
  • Accepted : 2016.03.16
  • Published : 2016.06.30
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Abstract

This study investigated the effect of exogenous hydrogen peroxide ($H_2O_2$) on the antioxidant responses and growth of warm-season turfgrass (Zoysia japonica Steud.) and cool-season turfgrass (Poa pratensis L.) subjected to drought stress. Compared with control plants that were not pretreated with $H_2O_2$, plants pretreated with $H_2O_2$ had significantly greater fresh and dry weights of shoots and roots, and increased water content. $H_2O_2$ pretreatments before drought stress significantly decreased the concentrations of malondialdehyde and $H_2O_2$. DPPH radical scavenging and glutathione activities were significantly increased. The responsive activities of the antioxidant enzymes superoxide dismutase, ascorbate peroxidase, catalase, and peroxidase were also significantly enhanced. Our results suggest that exogenous $H_2O_2$ could improve the growth of warm-season and cool-season turfgrass under drought stress by increasing the activity of their antioxidant enzymes, while decreasing lipid peroxidation.

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References

  1. Aebi H (1974) Catalase. In HU Bergmeyer, ed, Methods of enzymatic analysis. Academic Press, London, U.K., pp 673-677. doi:10.1016/B978-0-12-091302-2
  2. Azevedo Neto AD, Prisco JT, Eneas-Filho J, Medeiros JV, Gomes-Filho E (2005) Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. J Plant Physiol 162:1114-1422. doi:10.1016/j.jplph.2005.01.007
  3. Baier M, Dietz KJ (1998) The costs and benefits of oxygen for photosynthesizing plant cells. Prog Bot 60:282-314. doi:10.1007/978-3-642-59940-8_11
  4. Bartosz G (1997) Oxidative stress in plants. Acta Physiol Plant 19:47-64. doi:10.1007/s11738-997-0022-9
  5. Beard JB (1989) Turfgrass water stress: drought resistance components physiological mechanisms and species-genotypes diversity. Proc of the Sixth Int Turf Sci, Tokyo Japan, pp 23-28
  6. Beauchamp C, Fridovich I (1971) Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276-287. doi:10.1016/0003-2697(71)90370-8
  7. Blios MS (1958) Antioxidant determination by the use of stable free radical. Nature 26:1199-1202. doi:10.1038/1811199a0
  8. Carrow RN (1996) Drought avoidance characteristics of diverse tall fescue cultivars. Crop Sci 36:371-377. doi:10.2135/cropsci1996.0011183X003600020026x
  9. Chen GX, Asada K (1989) Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30:987-998.
  10. Chen Z, Wang ML, Waltz C, Raymer P (2009) Genetic diversity of warm-season turfgrass: Seashore paspalum, bermudagrass, and zoysiagrass by AFLPs. Floriculture & Ornamental Biotechnol 3:20-24.
  11. Chen ZL, Li XM, Zhang LH (2014) Effect of salicylic acid pretreatment on drought stress responses of zoysiagrass (Zoysia japonica). Russian J. Plant Physiol. 61:619-625. doi:10.1134/S1021443714050057
  12. Dat JF, Vandenbeele S, Vranova E, Montagu MV, Inze D, Berusegem FV (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779-795. doi:10.1007/s000180050041
  13. Fadzilla, N.M., R.P. Finch, and R.H. Burdon. (1997) Salinity, oxidative stress and antioxidant reponses in shoot cultures of rice. J Exp Bot 48:325-331. doi: 10.1093/jxb/48.2.325
  14. Farooq M, Wahid A, Kobayashi N, Fujita D, Basra S (2009) Plant drought stress: effects, mechanisms and management. Sustain. Agr. Springer, pp 153-188
  15. Foyer CH, Souriau N, Perret S, Lelandais M, Kunert KJ, Pruvost C, Jouanin L (1995) Over-expression of glutathione reductase but not glutathione synthetase leads to increase in antioxidant capacity and improved photosynthesis in poplar (Populus tremula $\times$ P. alba) tress. Plant Physiol 109:1047-1057. doi:10.1104/pp.109.3.1047
  16. Foyer CH, Looez-Delgado H, Dat J, Scott IM (1997) Hydrogen peroxide and glutathione-associated mechanisms of acclamatory stress tolerance and signaling. Physiol Plant 100:241-254. doi:10.1034/j.1399-3054.1997.1000205.x
  17. Foyer CH, Noctor G (2000) Oxygen processing in photosynthesis: regulation and signalling. New Phytol 146:359-388. doi: 10.1046/j.1469-8137.2000.00667.x
  18. Fridovich I (1975) Superoxide dismutase. Annu Rev Biochem 44:147-159. doi: 10.1146/annurev.bi.44.070175.001051
  19. Fu J, Huang B (2001) Involvement of antioxidants and lipid peroxidation in the adaption of two cool-season grasses to localized drought stress. Environ Exp Bot 45:104-114. doi:10.1016/S0098-8472(00)00084-8
  20. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909-930. doi:10.1016/j.plaphy.2010.08.016
  21. Gong M, Chen B, Li ZG, Guo LH (2001) Heat-shock-induced cross adaptation to heat, chilling, drought and salt stress in maize seedlings and involvement of $H_2O_2$. J Plant Physiol 158:1125-1130. doi:10.1078/0176-1617-00327
  22. Gong HJ, Zhu XY, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313-321. doi:10.1016/j.plantsci.2005.02.023
  23. Guri A (1983) Variation in Glutathion and ascorbic acid content among selected cultivars of Phaseolus vulgaris prior to and affect exposure to ozone. Can J Plant Sci 63:733-737. doi:10.4141/cjps83-090
  24. Heath RL, Pacher L (1968) Photo peroxidation in isolated chloroplast I. Kinetics and stoichemistry of fatty acid peroxidation. Arch Biochem Biophys 125:189-198. doi:10.1016/0003-9861(68)90654-1
  25. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:1-32
  26. Huang B, Duncan RR, Carrow RN (1997) Drought-resistance mechanisms of seven warm-season turfgrasses under surface soil drying: I. Shoot Response. Crop Sci 37:1858-1869. doi:10.2135/cropsci1997.0011183X003700060032x
  27. Jin R, Shi H, Han C, Zhong B, Wang Q, Chan Z (2015) Physiological changes of purslane (Portulaca oleracea L.) after progressive drought stress and rehydration. Scientia Horticulturae 194:215-221. doi:10.1016/j.scienta.2015.08.023
  28. Kranner I, Grill D (1996) Significance of thioldisulphide exchange in resting of plant development. Bot Acta 109:8-14. doi:10.1111/j.1438-8677.1996.tb00864.x
  29. Larkindale J, Huang B (2004) Thermotolerance and antioxidant systems in Agrostis stolonifera : involvement of salicylic acid, abscisic acid, calcium, hydrogen peroxide, and ethylene. J Plant Physiol 161: 405-413. doi:10.1078/0176-1617-01239
  30. Liang YC, Chen Q, Zhang WH, Ding RX (2003) Exogenous silicon (Si) increase antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160:1157-1164. doi:10.1078/0176-1617-01065
  31. Liu J, Xie X, Du J, Sun J, Bai X (2008) Effects of simultaneous drought and heat stress on Kentucky bluegrass. Sci Hortic 115:190-195. doi:10.1016/j.scienta.2007.08.003
  32. Nicolas, M.E., R.M. Gleadow, and M.J. Dalling. (1984) Effects of drought and high temperature on grain growth in wheat. Aust J Plant Physiol 11:553-566. doi:10.1071/PP9840553
  33. Noctor G, Strohm M, Jouanin L, Kuuert KJ, Foyer CH, Rennenberg H (1996) Synthesis of glutathione in leaves of transgenic popular (Populus tremula $\times$ P. alba ) over-expressing ${\gamma}$-glutamyl cystein synthetase. Plant physiol 112:1071-1078 https://doi.org/10.1104/pp.112.3.1071
  34. Perdomo P, Murphy JA, Berkowitz GA (1996) Physiological changes associated with performance of Kentucky bluegrass cultivars during summer stress. HortScience 31:1182-1186
  35. Reddy AR, Chaitanya KV, Vivekanandan M (2004) Drought induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189-1202. doi:10.1016/j.jplph.2004.01.013
  36. Rios JJ, Rosales MA, Blasco B, Cervilla LM, Romero L, Ruiz JM (2008) Biofortification of Se and induction of the antioxidant capacity in lettuce plants. Sci Hortic 116:248-255. doi:10.1016/j.scienta.2008.01.008
  37. Sadasivam S, Manickam A (1996) Biochemical methods. 2nd ed. New Age International Ltd. Publisher, New Delhi, India, pp 179-186
  38. Shekhawat GS, Verma K, Jana S, Singh K, Teotia P, Prasad A (2010) In vitro biochemical evaluation of cadmium tolerance mechanism in callus and seedlings of Brassica juncea. Protoplasma 239:31-38. doi:10.1007/s00709-009-0079-y
  39. Shi H, Ye T, Chan Z (2014) Comparative proteomic responses of two bermudagrass [Cynodon dactylon (L.) Pers.] varieties contrasting in drought stress resistance. Plant Physiol Biochem 82:218-228. doi:10.1016/j.plaphy.2014.06.006
  40. Shri M, Kumar S, Chakrabarty D, Trivedi PK, Mallick S, Misra P, Shukla D, Mishra S, Srivastava S, et al (2009) Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotoxicol Environ Saf 72:1102-1110. doi:10.1016/j.ecoenv.2008.09.022
  41. Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27-58. doi:10.1111/j.1469-8137.1993.tb03863.x
  42. Uchida A, Jagendorf AT, Hibino T, Takabe T (2002) Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Sci 63:515-523. doi:10.1016/S0168-9452(02)00159-0
  43. Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective some of exogenous polyamines. Plant Sci 151:59-66. doi:10.1016/s0168-9452(99)00197-1
  44. Wahid A, Perveen M, Gelani S, Basra SM (2007) Pretreatment of seed with $H_2O_2$ improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J Plant Physiol 164:283-294. doi:10.1016/j.jplph.2006.01.005
  45. Zhang X, Ervin EH, Schmidt RE (2005) The role of leaf pigment and antioxidant levels in UV-B resistance of dark-and light -green Kentucky bluegrass cultivars. J Am Soc Hortic Sci 130:836-841
  46. Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt stressed cucumber (Cucumis sativus L.). Plant Sci 167:527-533. doi:10.1016/j.plantsci.2004.04.020