Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Inhibition of cell wall synthesis in Cryptococcus neoformans and decrease of skin allergy induced with Alternaria alternata in mouse model by a chitinase from an inky cap

먹물버섯 키틴질 분해효소에 의한 인체유해성 Cryptococcus neoformans 세포벽 생성억제 및 Alternaria alternata에 기인한 생쥐의 피부알레르기의 감소

  • Kang, Yuri (Department of Biochemistry, Kangwon National University) ;
  • Choi, Hyoung T. (Department of Biochemistry, Kangwon National University)
  • 강유리 (강원대학교 자연과학대학 생화학과) ;
  • 최형태 (강원대학교 자연과학대학 생화학과)
  • Received : 2016.05.09
  • Accepted : 2016.06.13
  • Published : 2016.06.30
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Abstract

The growth of two different fungal species, human pathogenic Cryptococcus neoformans and plant pathogenic Alternaria alternata were inhibited by a chitinase (Chi2) expressed in the autolysing tissue of Coprinellus congregatus. The cell wall thickness was reduced (up to 32%) in C. neoformans compared with that of normal cell, and polysaccharide fibers located outside of the cell wall were also severely removed. The hyphal growth of A. alternata on agar plate was stopped by the enzyme. The allergic inflammation induced by A. alternata was reduced by the enzyme reaction when compared with untreated control in a mouse model.

먹물버섯 Coprinellus congregatus의 버섯조직이 자가분해되는 시기에 발현되는 chitinase (Chi2)는 사람에게 질병을 일으키는 Cryptococcus neoformans의 세포벽 두께를 정상 세포벽의 32% 수준까지 감소시킬 뿐만 아니라, 세포 외 섬유상 물질도 제거하였다. Chi2 처리에 의하여 사람에게 알레르기를 유발하는 Alternaria alternata의 생장은 물론 생쥐 모델에서 이 균에 의한 피부알레르기 증상도 감소시켰다.

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References

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