The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells

  • Moslehi, Akram (Department of Immunology, Medical School, Isfahan University of Medical Sciences) ;
  • Hashemi-beni, Batool (Department of Anatomical Science and Molecular Biology, Medical School, Isfahan University of Medical Sciences) ;
  • Moslehi, Azam (Department of Physiology & Pharmacology, Medical School, Qom University of Medical Sciences) ;
  • Akbari, Maryam Ali (Department of Anatomical Science and Molecular Biology, Medical School, Isfahan University of Medical Sciences) ;
  • Adib, Minoo (Department of Immunology, Medical School, Isfahan University of Medical Sciences)
  • Received : 2014.12.05
  • Accepted : 2015.03.17
  • Published : 2016.07.01
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Abstract

Membrane-bound HLA-G (mHLA-G) discovery on adipose derived stem cells (ADSCs) as a tolerogenic and immunosuppressive molecule was very important. Many documents have shown that HLA-G expression can be controlled via some hormones such as progesterone (P4) and estradiol (E2). Therefore, this study was designed to evaluate progesterone and estradiol effects on mHLA-G in ADSCs at restricted and combination concentrations. Three independent cell lines were cultured in complete free phenol red DMEM and subcultured to achieve sufficient cells. These cells were treated with P4, E2 and P4 plus E2 at physiologic and pregnancy concentrations for 3 days in cell culture conditions. The HLA-G positive ADSCs was measured via monoclonal anti HLA-G-FITC/ MEMG-09 by means of flow cytometry in nine groups. Data were analyzed by one way ANOVA and Tukey's post hoc tests. There were no significant values of the mean percentage of HLA-G positive cells in E2-treated and the combination of P4 plus $E_2-treated$ ADSCs compared to control cells (p value>0.05) but P4 had a significant increase on mHLA-G in ADSCs (p value<0.05). High P4 concentration increased mHLA-G but E2 and the combination of P4 plus E2 could not change mHLA-G on ADSCs.

Keywords

References

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