Biomaterials and Biomechanics in Bioengineering

  • 제3권1호
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  • Pages.59-69
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  • 2016
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  • 2465-9835(pISSN)
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  • 2465-9959(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

DOI QR Code

Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion

  • Chien, Hsiu-Wen (Department of Chemical Engineering, National Taiwan University) ;
  • Keng, Ming-Chun (Department of Chemical Engineering, National Taiwan University) ;
  • Chen, Hsien-Yeh (Department of Chemical Engineering, National Taiwan University) ;
  • Huang, Sheng-Tung (Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Science and Technology) ;
  • Tsai, Wei-Bor (Department of Chemical Engineering, National Taiwan University)
  • 투고 : 2015.10.01
  • 심사 : 2016.02.15
  • 발행 : 2016.03.25
⟨ 이전 논문 다음 논문 ⟩

초록

A surface resisting protein adsorption and cell adhesion is highly desirable for many biomedical applications such as diagnostic devices, biosensors and blood-contacting devices. In this study, a surface conjugated with sulfobetaine molecules was fabricated via the click reaction for the anti-fouling purpose. An alkyne-containing substrate (Alkyne-PPX) was generated by chemical vapor deposition of 4-ethynyl-[2,2]paracyclophane. Azide-ended mono-sulfobetaine molecules were synthesized and then conjugated on Alkyne-PPX via the click reaction. The protein adsorption from 10% serum was reduced by 57%, while the attachment of L929 cells was reduced by 83% onto the sulfobetaine-PPX surface compared to the protein adsorption and cell adhesion on Alkyne-PPX. In conclusion, we demonstrate that conjugation of mono-sulfobetaine molecules via the click chemistry is an effective way for reduction of non-specific protein adsorption and cell attachment.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Science and Technology of Taiwan

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피인용 문헌

  1. Conjugation of Polysulfobetaine via Poly(pyrogallol) Coatings for Improving the Antifouling Efficacy of Biomaterials vol.6, pp.5, 2016, https://doi.org/10.1021/acsomega.0c04643