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Stalk Rot Caused by Fusarium thapsinum on Sorghum at Organic Paddy-upland Rotation System in Korea

유기농 답전윤환지 Fusarium thapsinum에 의한 수수 줄기썩음병

  • Kim, Byung-Ryun (Agricultural Environment Division, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Han, Kwang-Seop (Agricultural Environment Division, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Hahm, Soo-Sang (Agricultural Environment Division, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Kang, Young-Sik (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Park, In-Hee (Agricultural Environment Division, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Yoon, Seong Tak (College of Bio-Resource Science, Dankook University)
  • 김병련 (충청남도농업기술원 농업환경과) ;
  • 한광섭 (충청남도농업기술원 농업환경과) ;
  • 함수상 (충청남도농업기술원 농업환경과) ;
  • 강영식 (충청남도농업기술원 작물연구과) ;
  • 박인희 (충청남도농업기술원 농업환경과) ;
  • 윤성탁 (단국대학교 생명자원과학대학)
  • Received : 2016.10.05
  • Accepted : 2016.11.05
  • Published : 2016.12.31

Abstract

In late June 2016, stalk rot symptoms were observed on five vatieties of sorghum (Sorghum bicolar) at organic paddy-upland rotation system in Anseong city, Korea. The initial symptom on stalk surfaces was red color with a dark red spot lesion. A fungus was isolated from the initial lesion, and cultured on potato dextrose agar. Size of microconidia mostly extend to $5-19{\times}2-{\mu}m$ in culture, with 0-1 septa and macroconidia extend to $29-52{\times}3-4{\mu}m$ with 4-6 septa. Pathogenicity was investigated using conidial suspension spray to seedling of sorghum. After 3 days of inoculation, the dark red lesion was produced on stalks. On the basis of mycological characteristics, pathogenicity, and internal transcribed spacer (ITS) rDNA sequence analysis, this fungus was identified as Fusarium thapsinum. This is the first report of stalk rot on sorghum caused by F. thapsinum in Korea.

2016년 6월 말 경기도 안성시의 유기농 답전윤환 시험연구포장의 수수 5개 품종에서 줄기썩음병 증상이 관찰되었다. 초기 병징은 줄기의 표면에 적색의 병반이 형성되고 내부에 흑갈색의 반점이 존재하였다. 이후 진전되면 병반 표면에 연한 오렌지색의 포자가 형성되었다. 병원균을 분리하여 배양하면 균총은 백색에서 연한 오렌지색 또는 연노란색을 나타내고 소형분생포자는 $5-19{\times}2-4{\mu}m$의크기로 1-2개의격막을, 대형 분생포자는 $29-52{\times}3-4{\mu}m$로 4-6개의 격막을 갖고 있었다. 병원균의 분생포자 현탁액으로 수수유묘에 분무접종 3일 후 줄기에 진한 적색의 병반이 형성되었고 병의 진전에 따라 줄기가 고사하여 식물체는 쓰러졌다. 분리된 균주의 균학적 특징, 병원성, rDNA ITS 영역의 염기서열을 기초로 병원균은 F. thapsinum으로 동정되었고, 수수의 줄기썩음병으로 명명하였다.

Keywords

References

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