Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory Effects of the Water Extract of Phyllostachys Folium via NF-κB Inhibition

죽엽 열수추출물의 염증억제 효과

  • Son, Jin Won (College of Korean Medicine, Daegu Haany University) ;
  • Park, Sang Mi (College of Korean Medicine, Daegu Haany University) ;
  • Jung, Ji Yun (College of Korean Medicine, Daegu Haany University) ;
  • Hwangbo, Min (College of Korean Medicine, Daegu Haany University) ;
  • Cho, Il Je (College of Korean Medicine, Daegu Haany University) ;
  • Jung, Tae Young (College of Korean Medicine, Daegu Haany University) ;
  • Park, Chung A (College of Korean Medicine, Daegu Haany University) ;
  • Kim, Sang Chan (College of Korean Medicine, Daegu Haany University) ;
  • Jee, Seon Young (College of Korean Medicine, Daegu Haany University)
  • 손진원 (대구한의대학교 한의과대학) ;
  • 박상미 (대구한의대학교 한의과대학) ;
  • 정지윤 (대구한의대학교 한의과대학) ;
  • 황보민 (대구한의대학교 한의과대학) ;
  • 조일제 (대구한의대학교 한의과대학) ;
  • 정태영 (대구한의대학교 한의과대학) ;
  • 박정아 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학) ;
  • 지선영 (대구한의대학교 한의과대학)
  • Received : 2016.09.29
  • Accepted : 2016.11.08
  • Published : 2016.11.30
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Abstract

Objectives : Phyllostachys Folium is leaves of Phyllostachys nigra var. henesis $S_{TAPF}$. In the East Asian traditional medicine, the herb has been used to treat nasal bleeding, dysuria, epilepsy and etc. The present study was conducted to evaluate the anti-inflammatory effects of the Phyllostachys Folium water extracts (PFE) in vitro and in vivo model. Methods : Cell viability was measured by MTT assay after the treatment of PFE and NO production was monitored by measuring the nitrite content in culture medium. iNOS, COX-2, $I{\kappa}B$, $p-I{\kappa}B{\alpha}$ amd $NF{\kappa}B$ were detected by immunoblot analysis, and levels of cytokine were analyzed by sandwich ELISA kit. Anti-edema effect of PFE was determined in the carrageenan-induced paw edema model in rats. Results : LPS increased NO and cytokines levels compared with control, these increases were attenuated by PFE. In addition, LPS-induced pro-inflammatory proteins such as iNOS, COX-2 were down regulated by PFE. These anti-inflammatory effect of PFE results from inhibition of phosphorylation of $I{\kappa}B$ and translocation of $NF-{\kappa}B$. Conclusion : These results show that PFE has some anti-inflammatory effects which might play a role in gram-negative bacterial infection inflammation and $NF{\kappa}B$ activated diseases.

Keywords

References

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