Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Cytogenetic Study of Diploid and Triploid Marine Medaka, Oryzias dancena

해산송사리, Oryzias dancena 유도 3배체의 세포유전학적 연구

  • Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Gil, Hyun Woo (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Lee, Tae Ho (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Nam, Yoon Kwon (Department of Marine and Aquaculture, Pukyung National University) ;
  • Ko, Min Gyun (Department of Marine and Aquaculture, Pukyung National University) ;
  • Kim, Dong Soo (Department of Marine and Aquaculture, Pukyung National University)
  • 박인석 (한국해양대학교 해양생명과학부) ;
  • 길현우 (한국해양대학교 해양생명과학부) ;
  • 이태호 (한국해양대학교 해양생명과학부) ;
  • 남윤권 (부경대학교 해양바이오신소재공학과) ;
  • 고민균 (부경대학교 해양바이오신소재공학과) ;
  • 김동수 (부경대학교 해양바이오신소재공학과)
  • Received : 2016.10.19
  • Accepted : 2016.12.13
  • Published : 2016.12.31
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Abstract

Triploidy was induced in the marine medaka, Oryzias dancena by cold shock treatment ($0^{\circ}C$) of fertilized eggs for 30, 45, or 60 min, applied two minutes after fertilization. The triploid genotype was induced by each of the thermal shock regimes tested. The best result was obtained when the eggs were treated for 45 min, which induced triploidy in all the resulting fish. Triploidy was confirmed using chromosomal and flow cytometer analyses, and erythrocyte measurements. The surface areas and volumes of the erythrocytes of triploid fish were significantly larger than those of diploid fish, and their chromosome number (3N=72) was 1.5 times greater that for the diploids (2N=48). Based on a flow cytometer analysis, the triploid fish had approximately 1.5 times the cellular DNA content (2.40 pg/cell) of the diploid specimens (1.61 pg/cell). Data from this study provide the basis for the development of unique models for studying reproductive confinement in transgenic fish.

본 연구는 3배체 해산송사리, Oryzias dancena를 생산하기 위해서 다양한 조건에서 실험을 실시하였다. 저온처리($0^{\circ}C$)에서 30, 45, 60분간 처리하여 3배체를 유도 생산하였다. 여러 유도조건 결과, 45분간 처리하였을 때 가장 높은 3배체 생산율이 나타났다. 3배체 판별은 Chromosome 관찰, flow cytometer 분석 및 적혈구 측정을 통해서 판별하였다. 3배체 해산송사리의 적혈구 표면적과 부피는 2배체 해산송사리보다 크게 나타났고, 3배체 Chromosome number는 72개, 2배체는 48개가 관찰되었다. Flow cytometer 분석에서도 3배체가 2.40 pg/cell 그리고 2배체가 1.61 pg/cell 측정되어 DNA contents도 3배체가 2배체보다 1.5배 정도 크게 관찰되었다. 본 연구 결과는 불임 형질전환 어류를 위한 실험동물로의 3배체 해산송사리의 유용성 및 가치성을 제공한다.

Keywords

References

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