Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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An Outbreak of Gregarious Nymphs of Locusta migratoria (Orthoptera: Acrididae) in Korea and Their Genetic Lineage Based on mtDNA COI Sequences

한국에서 군집형 풀무치의 대발생과 그 집단의 유전적 계통

  • Lee, Gwan Seok (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Kwang Ho (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Chang Seok (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Wonhoon (Department of Plant Medicine and Institute of Agriculture & Life Science, Gyeongsang National University)
  • 이관석 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 김광호 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 김창석 (국립농업과학원 농산물안전성부 작물보호과) ;
  • 이원훈 (경상대학교 식물의학과(농업생명과학연구원))
  • Received : 2016.11.06
  • Accepted : 2016.11.16
  • Published : 2016.12.01
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Abstract

The migratory locust Locusta migratoria, one of the world's most notorious insect pests, has polyphenic (gregarious or solitarious) characteristics. Although this species is known to have several morphological variants, it is genetically divided into two different lineages using mitochondrial genome analysis: Southern (Africa, Southern Europe, Southern Asia, and Australia) and Northern (East Asia and the Eurasian continent). In 2014, a large number of orange black-colored gregarious L. migratoria nymphs suddenly appeared at Haenamgun, Jeollanamdo in the south of Korea. This is the first report of gregarious phase locusts occurring in Korea. In this study, mitochondrial COI sequences of one nymph and 11 adults of L. migratoria were analyzed to examine the genetic lineage of the gregarious nymphs of L. migratoria. Our results showed that all 12 individuals belong to the Northern linage and have low intraspecific genetic divergences (0.0% - 0.9%).

풀무치(Locusta migratoria)는 벼과 작물(벼, 옥수수 등)에 큰 피해를 주는 세계적으로 유명한 메뚜기목 해충의 하나로서 밀도요인에 따라 형태와 행동적으로 구별되는 단독형(solitaria)과 군집형(gregaria)을 나타낸다. 풀무치는 전세계적으로 다양한 형태적인 변이가 알려져 있으나 최근의 분자생물학적 연구에 따르면 2가지 계통, 즉 남방계통(아프리카, 남유럽, 동남아시아, 호주)과 북방계통(동아시아, 유라시아)으로 나뉜다. 2014년 8월 전남 해남군 산이면에서 군집형 약충들이 대발생하여 주변 잡초 및 작물(벼, 기장)에 큰 피해를 주었다. 우리나라에서 군집형 풀무치의 발생에 대한 명확한 학술적 보고는 이번이 처음이다. 해남지역의 풀무치 대발생 지점에서 채집한 풀무치 12개체의 COI 염기서열을 분석한 결과, 0.0%-0.9%의 유전적 변이를 보였으며, 모두 북방계통에 속했다.

Keywords

References

  1. Chapuis, M.-P., Lecoq, M., Michalakis, Y., Loiseau, A., Sword, G.A., Piry, S., Estoup, A., 2008. Do outbreaks affect genetic population structure? A worldwide survey in Locusta migratoria, a pest plagued by microsatellite null alleles. Mol. Ecol. 17, 3640-3653. https://doi.org/10.1111/j.1365-294X.2008.03869.x
  2. COPR, 1982. The locust and grasshoppers agricultural manual. Centre for Overseas Pest Research, London.
  3. Farrow, R.A., Colless, D.H., 1980. Analysis of the interrelationships of geographical races of Locusta migratoria (Linnaeus) (Orthoptera: Acrididae) by numerical taxonomy, with special reference to sub-speciation in the tropics and affinities of the Australian race. Acrida 9, 77-99.
  4. Folmer, O., Black, M., Hoew, W., Lutz, R., Vrijenhoek, R., 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Marine Biol. Biotechnol. 3, 294-299.
  5. Hakomori, T., Tanaka, S., 1992. Genetic control of diapause and other developmental traits in Japanese strains of the migratory locust, Locusta migratoria L.: univoltine vs. bivoltine. Jap. J. Entomol. 60, 319-328.
  6. Herbert, P.D.N., Cywinska, A., Ball, S.L., DeWaard J.R., 2003. Biological identifications through DNA barcodes. Proc. Roy. Soc. 270, 313-321. https://doi.org/10.1098/rspb.2002.2218
  7. Ito, Y., Yamagishi, M., 1976. Outbreaks and partial phase transformation of Locusta migratoria L. in sugarcane fields of Minami-and Kita-Daito Zima, Okinawa. Acrida 5, 17-26.
  8. Kang, L., Chen, Y.L., 1992. An overview of locust catastrophe with special references to its research contents and analysis of the plague trends. Trans. Ecol. Soc. Chinese Youths. 2, 56-64.
  9. Kimura, M., 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide-sequences. J. Mol. Evol. 16, 111-120. https://doi.org/10.1007/BF01731581
  10. Kumar, R., Khan, Z.H., Ramaurthy, V.V., 2009. Tropical outbreak of migratory locust, Locusta migratoria migratorioides (Reiche & Fairmaire) in Ladakh valley of Jammu and Kashmir. Biological Forum-An International Journal 1, 107-109.
  11. Launois, M., 1974. Influence du facteur pluviometrique sur l'evolution saisonniere du Criquet migrateur en phase solitaire et sur sa gregarisation a Madagascar. Ministere de la cooperation, Paris. 159.
  12. Lecoq, M., 1974. Report of expert on a visit to Mali and the Lake Chad basin. African Migratory Locust Project RAF /69/146 Biological studies. UNDP(SF)AML/BIO/5. United Nations Development Programme, Food and Agriculture Organization of the United Nations, Rome. 32.
  13. Lecoq, M., 1991. The Migratory Locust, Locusta migratoria, in Africa and Malagasy. The Orthopterists' Society Field Guide Series, C2E. Orthopterists' Society, Ste-Anne de Bellevue, Quebec, Canada. 28.
  14. Lecoq, M., 1995. Forecasting systems for migrant pests. III. Locusts and grasshoppers in Africa and Madagascar. 377-395. IN: Drake VA, Gatehouse AG (eds), Insect migration: physical factors and physiological mechanisms. Cambridge University Press, Cambridge.
  15. Lecoq, M., Sukirno, 1999. Drought and an exceptional outbreak of the oriental migratory locust, Locusta migratoria manilensis (Meyen 1835) in Indonesia (Orthoptera: Acrididae). J. Orthoptera Resear. 8, 153-161.
  16. Lovejoy, N.R., Mullen, S.P., Sword, G.A., Chapman, R.F., Harrison, R.G., 2006. Ancient trans-Atlantic flight explains locust biogeography: molecular phylogenetics of Schistocerca. Proc. R. Soc. Lond. B Biol. Sci. 273, 767-774. https://doi.org/10.1098/rspb.2005.3381
  17. Ma, C., Yang P., Jiang, F., Chapuis, M.-P., Shall, Y., Sword, G.A., Kang, L., 2012. Mitochondrial genomes reveal the global phylogeography and dispersal routes of the migratory locust. Mol. Ecol. 21, 4344-4358. https://doi.org/10.1111/j.1365-294X.2012.05684.x
  18. Moulton, M.J., Song, H., Whiting, M.F., 2010. Assessing the effects of primer specificity on eliminating numt coamplification in DNA barcoding: a case study from Orthoptera (Arthropoda: Insecta). Mol. Ecol. Resour. 10, 615-627. https://doi.org/10.1111/j.1755-0998.2009.02823.x
  19. Muramtsu, S., 1921. The control of biwhang occurring in Joseon. J. Plant Diseas. Pest 8, 241-245, 302-305 (in Japanese).
  20. Paik, W.H., 1976. Records of brown planthoppers in the Annals of Joseon-Dynasty. Kyujanggak 1, 1-12 (in Korean).
  21. Park, H.C., Young, M.J., Lee Y.B., Lee, G.S., Kang, T.H., Han, T.M., Hwan, S.J., and Kim, T.W., 2010. Biological identity of hwangchung and history on the control of hwangchung outbreaks in Joseon Dynasty analyzed through the database program on the Annals of the Joseon Dynasty and the Enrollment of Haegoeje. Korean J. Appl. Entomol. 49, 375-384 (in Korean). https://doi.org/10.5656/KSAE.2010.49.4.375
  22. Pener, M.P., 1991. Locust phase polymorphism and its endocrine relations. Adv. Insect Physiol. 23, 1-79. https://doi.org/10.1016/S0065-2806(08)60091-0
  23. Song, H., Buhay, J.E., Whiting, M.F., Crandall, K.A., 2008. Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proc. Natl. Acad. Sci. U.S.A. 105, 13486-13491. https://doi.org/10.1073/pnas.0803076105
  24. Tanaka, H., 1994a. Geographic variation of embryonic diapause in the migratory locust, Locusta migratoria (Orthoptera: Acrididae), in Japan. Jap. J. Entomol. 62, 629-639.
  25. Tanaka, S., 1994b. Diapause as a pivotal factor for latitudinal and seasonal adaptation in Locusta migratoria in Japan. In: Danks HV, ed. Insect life-cycle polymorphism. Dordrecht: Kluwer Aca. Pub. 173-190.
  26. Tanaka, S., Zhu, D.H., 2008. Geographic variation in embryonic diapause, cold-hardiness and life cycles in the migratory locust Locusta migratoria (Orthoptera : Acrididae) in China. Entomol. Sci. 11, 327-339. https://doi.org/10.1111/j.1479-8298.2008.00284.x
  27. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731-2739. https://doi.org/10.1093/molbev/msr121
  28. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  29. Tokuda, M., Tanaka, S., Zhu, D.H., 2010. Multiple origins of Locusta migratoria (Orthoptera: Acrididae) in the Japanese Archipelago and the presence of two major clades in the world: evidence from a molecular approach. Bio. J. Linn. Soc. 99, 570-581. https://doi.org/10.1111/j.1095-8312.2010.01386.x
  30. Verdier, M., 1972. The different life cycles in Locusta in relation to climatic and genetic diversity. In: Hemming CF, Taylor THC, eds. Proceedings of the International Study Conference of Current and Future Problems of Acridology; 6-16 July 1970, London. London: Centre for Overseas Pest Research, 335-338.
  31. Uichanco L.B. 1936. Secular trends of locust outbreaks in the Philippines and their apparent relation with sunspot cycles. Philipp. J. Agric. 25, 321-354.
  32. Uvarov, B.P., 1921. A revision of the genus Locusta L. (=Pachytylus Fieb.), with a new theory as to the periodicity and migrations of locusts. Bull. Entomol. Res. 12, 135-163. https://doi.org/10.1017/S0007485300044989
  33. Uvarov, B., 1966. Grasshoppers and locusts, Vol. 1. Cambridge University Press, Cambridge, UK.
  34. Uvarov, B., 1977. Grasshoppers and locusts, Vol. 2. Centre for Overseas Pest Research, London.
  35. Yamagishi, M., Tanaka, S., 2009. Overwintering biology and morphological characteristics of the migratory locust, Locusta migratoria after outbreaks on Iheya Island, Japan. Appl. Entomol. Zool. 44, 165-174. https://doi.org/10.1303/aez.2009.165
  36. Zhang, Z., Li, D., 1999. A possible relationship between outbreaks of the oriental migratory locust (Locusta migratoria manilensis Meyen) in China and the El Nino episodes. Ecol. Res. 14, 267-270. https://doi.org/10.1046/j.1440-1703.1999.t01-1-143305.x