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Study on Sawdust Bag Cultivation of Shiitake (Lentinula edodes), using Oak Wilt-Diseased Logs

참나무시들음병 피해목을 이용한 표고 톱밥재배에 대한 연구

  • Kim, Sehun (Wood Chemistry and Microbiology Division, National Institute of Forest Science) ;
  • Seo, Sooyoung (Wood Chemistry and Microbiology Division, National Institute of Forest Science) ;
  • Jang, Yeongseon (Wood Chemistry and Microbiology Division, National Institute of Forest Science) ;
  • Ryoo, Rhim (Wood Chemistry and Microbiology Division, National Institute of Forest Science) ;
  • Seo, Sang-Tae (Forest Insect Pests and Diseases Division, National Institute of Forest Science) ;
  • Ka, Kang-Hyeon (Wood Chemistry and Microbiology Division, National Institute of Forest Science)
  • 김세훈 (국립산림과학원 화학미생물과) ;
  • 서수영 (국립산림과학원 화학미생물과) ;
  • 장영선 (국립산림과학원 화학미생물과) ;
  • 유림 (국립산림과학원 화학미생물과) ;
  • 서상태 (국립산림과학원 산림병해충연구과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Received : 2016.11.10
  • Accepted : 2016.12.07
  • Published : 2016.12.30

Abstract

Recently, the incidence of oak wilt disease has been increasing in Korea, resulting in an increasing number of dead trees. In this study, we performed sawdust bag cultivation of shiitake (Lentinula edodes) using oak wilt-diseased logs and measured the antioxidant activities of the resulting mushrooms. For sawdust media, 4 types of logs (healthy, infected, dead, and fumigated ones) were used. As inocula, three strains of L. edodes (NIFoS 764, Sanjo 701ho, and Sanmaru 2ho) were used. The productivity of L. edodes from dead logs was similar to that from healthy logs. Except for the Sanmaru 2ho strain, fumigated logs and infected logs yielded a lower productivity of L. edodes compared to healthy logs. In terms of antioxidant activity, L. edodes grown on sawdust from fumigated logs showed higher values than those from other logs.

본 연구에서는 참나무시들음병 피해목을 이용해서 표고 톱밥재배 가능성을 알아보고 생산된 표고의 항산화 활성을 알아보았다. 총 3개 균주와 4가지 톱밥재료를 이용하여 실험을 진행하였으며, 생산량 검정 결과 고사목을 이용하여 재배한 모든 균주는 건전목과 통계적으로 비슷한 생산량을 나타냈으며 산마루2호를 제외하고 훈증목과 고사진행목을 이용하였을 때 건전목보다 생산성이 떨어지는 것으로 확인되었다. 각 처리구에서 수확한 표고의 항산화 활성 측정 결과는 총 폴리페놀 함량과 DPPH 라디칼 소거능, FRAP, reducing power에서 훈증목이 다른 배지재료에 비해서 항산화 활성이 유의적으로 높게 나타났다. 본 연구에서 사용된 참나무시들음병 피해목의 이용 증진을 위해 추가적인 연구가 필요하며 참나무시들음병 피해목을 이용한 표고 톱밥재배 가능성과 생산된 표고의 항산화 활성을 확인함으로써 향후 참나무시들음병 피해목을 활용한 톱밥재배 관련 기초 자료로 활용될 수 있을 것으로 생각된다.

Keywords

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