Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of plant growth regulators and carbon source on the shoot regeneration and rooting of 'Wonhwang' pear (Pyrus pyrifolia L.)

배 '원황'의 재분화와 발근에 미치는 식물생장조절물질과 탄소원의 영향

  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Park, Seo Jun (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lee, Han Chan (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 김세희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 박서준 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 조강희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 이한찬 (농촌진흥청 국립원예특작과학원 과수과)
  • Received : 2016.11.04
  • Accepted : 2016.11.11
  • Published : 2016.12.31
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Abstract

The aim of this research is to establish shoot regeneration system for 'Wonhwang' pear (Pyrus pyrifolia L.) using various concentrations of 1-Naphthaleneacetic acid (NAA) 0.01, 0.05, 0.1, 0.5 mg/L in combination with benzylaminopurine (BA) 3, 5, 10 mg/L. Medium containing 4.4 g/L of Murashige and Skoog (MS) medium with vitamins containing 8 g/L of plant agar and 30 g/L of sucrose with NAA 0.05 mg/L and BA 3 mg/L showed 13.3% of shoot regeneration rate. 'Wonhwang' showed no root growth on existing rooting media of P. pyrifolia cv. Niitaka, 'Whang-keumbae' and 'Bae Yun No. 3'. We evaluated the effect of concentration and kinds of plant growth regulators and carbon source to establish efficient rooting condition for 'Wonhwang' pear. In the result of using various concentrations of NAA 0.5 mg/L and 1.0 mg/L in combination with indolebutyric acid (IBA) 3, 5, 10 mg/L, rooting rate of 24% was observed using 1/4 Linsmaier and Skoog (LS) medium supplemented with 7.5 g/L glucose as carbon source and IBA 1.0 mg/L with NAA 1.0 mg/L.

본 연구의 목적은 배 '원황'의 효율적인 재분화 조건을 규명하기 위해 NAA 0.01, 0.05, 0.1, 0.5 mg/L과 BA 3, 5, 10 mg/L을 조합하여 처리한 결과, NAA 0.05 mg/L, BA 3 mg/L의 조성에 plant agar 8 g/L와 sucrose 30 g/L의 MS 배지에서 재분화율이 13.3%로 나왔다. 기존의 '신고'와 '황금배'의 발근에 사용하였던 배지 조성과 '배연3호'의 발근 배지조성에서는 '원황' 품종의 발근이 전혀 이뤄지지 않았다. 식물생장조절물질이 '원황'의 발근에 미치는 영향을 알아보기 위하여 NAA 0.5, 1.0 mg/L, IBA 0.3, 0.5, 1.0 mg/L을 조합하여 처리한 결과 IBA 1.0 mg/L와 NAA 1.0 mg/L의 조합에 1/4 LS vitamin이 포함된 배지에서 발근률이 24%로 나타났으며 효율적인 '원황'의 발근을 위해서는 탄소원으로 glucose 7.5 g/L가 사용되었다.

Keywords

References

  1. Brown DCW, Leung DWM, Thorpe TA (1979) Osmotic requirement for shoot formation in tabacco callus. Physiol Plant 46:36-41 https://doi.org/10.1111/j.1399-3054.1979.tb03182.x
  2. Challice JS, Westwood MN (1973) Numerical taxonomic studies of the genus Pyrus using both chemical and botanical characters. Bot J Linn Soc 67:121-148 https://doi.org/10.1111/j.1095-8339.1973.tb01734.x
  3. Cheng TY (1979) Micropropagation of clonal fruit tree rootstocks. Compact Rruit Trees 12:127-137
  4. Chevreau E, Skirvin RM, Abu-Qaoud HA, Korban SS, Sullivan JG (1989) Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro. Plant Cell Rep 7:688-691
  5. Chevreau E, Leblay C (1993) The effect of mother plant pretreatment and explants choice on regeneration from in vitro pear leaves. Acta Hort 336:263-268
  6. Chun JA, Do KR, Kim SH, Cho KH, Kim HR, Hwang HS, Shin IS (2012) In vitro shoot regeneration from leaf tissue of 'Whangkeumbae' pear (Pyurs pyrifolia Nakai) J Plant Biotechnol 39:288-294 https://doi.org/10.5010/JPB.2012.39.4.288
  7. Hennayake CK, Dissanayake K, Matsuda N, Takasaki T, Nakanishi T (2003) An Efficient and reproducible in vitro plant regeneration from leaf discs in pear cultivars (Pyrus spp). Plant Biotechnol 20:283-289 https://doi.org/10.5511/plantbiotechnology.20.283
  8. Huetteman CA, Preece JE (1993) Thidiazuron: A potent cytokine for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105-119 https://doi.org/10.1007/BF01983223
  9. Kim SH, Shin IS, Cho KH, Kim DH, Kim HR, Kim KO, Lee HB, Do KR, Chun JA, Hwang HS (2013) Shoot regeneration via culture of leaf explants in pear (Pyrus pyrifolia cv. Niitaka). J Plant Biotechnol 40:203-209 https://doi.org/10.5010/JPB.2013.40.4.203
  10. Lane WD (1979) Regeneration of pear plants from shoot meristem-tips. Plant Sci Lett 16:337-342 https://doi.org/10.1016/0304-4211(79)90046-4
  11. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100-127 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
  12. Matsuda N, Gao M, Isuzugawa K, Takashina T, Nishimura K (2005) Development of an Agrobacterium-mediated transformation method of pear (Pyrus communis L.) with leaf-section and axillary shoot-meristem explants. Plant Cell Rep 24:45-51 https://doi.org/10.1007/s00299-005-0924-1
  13. Moon JG, Choo BK, Doo HS, Kwon TH, Yahn MS, Ryu JH (2000) Effect of growth regulators on plant regeneration from the cotyledon explants in oriential melon (Cucumis melo L.). Kor J Plant Tiss Cult 27:1-6
  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Phyiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Nakajima I, Sato Y, Saito T, Moriguchi T, Yamamoto T (2013) Agrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia). Breeding Science 63:275-283 https://doi.org/10.1270/jsbbs.63.275
  16. Ochatt SJ, Power JB (1988) Plant regeneration from mesophyll protoplasts of Williams' Bon Chretien (syn. Bartlett) pear (Pyrus communis L.). Plant Cell Rep 7:587-589 https://doi.org/10.1007/BF00272764
  17. Sepahvand S, Ebadi A, Kamali K, Ghaemmaghami SA (2012) Effects of myo-inositol and thaiamine on micropropagation of GF677 (Peach${\times}$Almond Hybrid). J Agricult Sci 4:275-280
  18. Singha S (1984) Influence of two commercial agars on in vitro shoot proliferation of 'Almey' crabapple and Pyrus communis 'Seckel'. Hort Science 19:227-228
  19. Sun Q, Sun H, Bell RL, Li H, Xin L (2011) Variation of phenotype, ploidy level, and organogenic potential of in vitro retgenerated polyploids of Pyrus communis. Plant Cell Tissue Organ Cult. doi:10.1007/s11240-011-9965-z
  20. Tukey HB (1934) Artificial culture methods for isolated embryos of deciduous fruits. Proc Am Soc Hort Sci 32:630-665
  21. Yotsuya D (1980) Effects of $GA_{4+7}$ and BA on the in vitro growth of 'Kosui' leaf bud. Sci Rep Fac Kobe Univ 14:45-49

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