Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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L-DOPA Synthesis Using Tyrosinase-immobilized on Electrode Surfaces

  • Rahman, Siti Fauziyah (Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University) ;
  • Gobikhrisnan, Siramulu (Department of Bioscience and Technology, Karunya University) ;
  • Gozan, Misri (Bioprocess Engineering Research Group, Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia) ;
  • Jong, Gwi Taek (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Park, Don-Hee (Department of Biotechnology and Bioengineering, Chonnam National University)
  • Received : 2016.09.21
  • Accepted : 2016.10.25
  • Published : 2016.12.01
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Abstract

Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the direct precursor of the neurotransmitter dopamine. L-DOPA is a well-known neuroprotective agent for the treatment of Parkinson's disease symptoms. L-DOPA was synthesized using the enzyme, tyrosinase, as a biocatalyst for the conversion of L-tyrosine to L-DOPA and an electrochemical method for reducing L-DOPAquinone, the product resulting from enzymatic synthesis, to L-DOPA. In this study, three electrode systems were used: A glassy carbon electrode (GCE) as working electrode, a platinum, and a Ag/AgCl electrode as auxiliary and reference electrodes, respectively. GCE has been modified using electropolymerization of pyrrole to facilitate the electron transfer process and immobilize tyrosinase. Optimum conditions for the electropolymerization modified electrode were a temperature of $30^{\circ}C$ and a pH of 7 producing L-DOPA concentration 0.315 mM. After 40 days, the relative activity of an enzyme for electropolymerization remained 38.6%, respectively.

Keywords

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