Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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A Comparison of Spinosin Content in Zizyphi Semen and Its Processed Products by Roasting

산조인의 수치에 따른 Spinosin 함량 비교

  • Seo, Chang-Seob (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Byoung-Soo (Department of Physiology, College of Korean Medicine, Daejeon University)
  • 서창섭 (한국한의학연구원 K-herb연구단) ;
  • 김정훈 (한국한의학연구원 K-herb연구단) ;
  • 신현규 (한국한의학연구원 K-herb연구단) ;
  • 김병수 (대전대학교 한의과대학)
  • Received : 2016.10.19
  • Accepted : 2016.12.12
  • Published : 2016.12.30
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Abstract

The aim of this study was to compare the amount of spinosin in the 70% ethanol extracts of non-processed Zizyphi Semem (ZS) and processed ZS by roasting using a high-performance liquid chromatography equipped with photodiode array detector. Separation of the spinosin was used $SunFire^{TM}$ $C_{18}$ analytical column ($5{\mu}m$, $4.6{\times}150mm$) using two mobile phase consisting of distilled water and acetonitrile, both with 1.0% (v/v) acetic acid. The flow rate was 1.0 mL/min and injection volume was $10{\mu}L$. Calibration curve of the spinosin was y = 22339.45x+483.99 in tested concentration range ($1.28-20.00{\mu}g/mL$) and correlation coefficient was 1.0000. In non-processed ZS sample, the amount of the spinosin was 0.94 m/g, while, the amount of the marker compound in processed ZS samples were 0.66-1.10 mg/g.

Keywords

References

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