Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of a Multiplex PCR for Discrimination of the TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor Strains

  • Kim, Eun Jin (Department of Pharmacy, College of Pharmacy, Hanyang University) ;
  • Yu, Hyun Jin (Department of Pharmacy, College of Pharmacy, Hanyang University) ;
  • Nair, G. Balakrish (South East Asia Regional Office, World Health Organization) ;
  • Kim, Dong Wook (Department of Pharmacy, College of Pharmacy, Hanyang University)
  • Received : 2016.07.08
  • Accepted : 2016.08.30
  • Published : 2016.12.28
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Abstract

Vibrio cholerae O1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains.

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References

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