Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Characterization of the IgA Fc Receptor from Swine

  • Chen, Yumei (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Liu, Yunchao (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Zhang, Gaiping (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Feng, Hua (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Ji, Pengchao (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Wang, Guoqiang (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Liu, Chang (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Song, Yapeng (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Su, Yunfang (Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences) ;
  • Qiao, Songlin ;
  • Wang, Aiping (School of Life Sciences, Zhengzhou University)
  • Received : 2016.07.05
  • Accepted : 2016.08.25
  • Published : 2016.12.28
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Abstract

The myeloid-specific IgA Fc receptor ($Fc{\alpha}R$) is a cell surface molecule on immunocytes that provides a fundamental connection between humoral and cellular immunity. In this study, the full-length cDNA sequence of swine $Fc{\alpha}RI$ ($swFc{\alpha}RI$) was isolated and characterized and found to contain a 792-base-pair open reading frame, encoding a 264-amino-acid transmembrane glycoprotein with a predicted molecular mass of 29.4 kDa. The $swFc{\alpha}RI$ shares high amino acid sequence homology (>50%) with its counterparts from cattle, seal, and horse. Rosetting analysis confirmed that COS-7 cells transfected with an $swFc{\alpha}RI$ expression plasmid was able to combine with chicken erythrocytes sensitized with porcine IgA, but not IgG.

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References

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