International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지
DOI QR코드

DOI QR Code

Exosome isolation from hemolymph of white-spotted flower chafer, Protaetia brevitarsis (Kolbe) (Coleoptera: Scarabaeidae).

  • Lee, Seokhyun (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Kisang (Department of Biomedical Laboratory Science, College of Health and Welfare, Kyungwoon University) ;
  • Song, Myung-Ha (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Kwan-ho (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University) ;
  • Choi, Ji-young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2016.10.17
  • Accepted : 2016.11.04
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Exosomes are homogenous vesicles of 40-100 nm diameter produced endogenously. Exosomes are generated by inward budding into multi-vesicular bodies (MVB) and then released to extracellular space. Exosomes contain various nucleic acid and protein cargoes from their cells of origin and this endosomal cellular molecules are used for intracellular communication and for both promotion and suppression of immune responses. Recently, they are also considered as delivery vehicle for therapeutic proteins due to their characteristics of stability in body fluids and ability for target uptake. Also, they show less immune reactivity because the isolated exosome harboring therapeutic proteins can be from the same host. White-spotted flower chafer, Protaetia brevitarsis is one of the major insect commercially reared in Korea. There are bacterial and fungal pathogens causing diseases in the beetle, and these diseases incur economic loss to the larva-rearing farms. Due to their endosomal cargoes, exosomes are good candidates in use of disease diagnosis. In this study, we isolated insect exosome from the hemolymph of P. brevitarsis, and verified it by analysis of the exosome-specific surface proteins and RNA.

Keywords

References

  1. Aalberts M, Sostaric E, Wubbolts R, Wauben MWM, Nolte ENM, Gadella BM, et al. (2013) Spermatozoa recruit prostasomes in response to capacitation induction. Biochim Biophys Acta 1834, 2326-2335. https://doi.org/10.1016/j.bbapap.2012.08.008
  2. An Q, Huckelhoven R, Kogel KH, van Bel AJE (2006) Multivesicular bodies participate in a cell wall-associated defence response in barley leaves attacked by the pathogenic powdery mildew fungus. Cell Microbiol 8, 1009-1019. https://doi.org/10.1111/j.1462-5822.2006.00683.x
  3. An Q, van Bel AJE, Huckelhoven R (2007) Do plant cells secrete exosomes derived from multivesicular bodies? Plant Signal Behav 2, 4-7. https://doi.org/10.4161/psb.2.1.3596
  4. Belov L, Matic KJ, Hallal S, Best OG, Mulligan SP, Christopherson RI (2016) Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples. J Extracell Vesicles 5, 25355. https://doi.org/10.3402/jev.v5.25355
  5. Bhatnagar S, Shinagawa K, Castellino FJ, Schorey JS (2007) Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo. Blood 110, 3234-3244. https://doi.org/10.1182/blood-2007-03-079152
  6. Chaput N, Thery C (2011) Exosomes: immune properties and potential clinical implementations. Semin Immunopathol 33, 419-440. https://doi.org/10.1007/s00281-010-0233-9
  7. Clayton A, Turkes A, Navabi H, Mason MD, Tabi Z (2005) Induction of heat shock proteins in B-cell exosomes. J Cell Sci 118, 3631-3638. https://doi.org/10.1242/jcs.02494
  8. El Andaloussi S, Mager I, Breakefield XO, Wood MJ (2013) Extracellular vesicles: biology and emerging therapeutic opportunities. Nat Rev Drug Discov 12, 347-357. https://doi.org/10.1038/nrd3978
  9. Gonorazky G, Laxalt AM, Dekker HL, Rep M, Munnik T, Testerink C, et al. (2012) Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids. Plant Biol 14, 41-49.
  10. Gyorgy B, Szabo TG, Pasztoi M, Pal Z, Misjak P, Aradi B, et al. (2011) Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles. Cell Mol Life Sci 68, 2667-2688. https://doi.org/10.1007/s00018-011-0689-3
  11. Ju S, Mu J, Dokland T, Zhuang X, Wang Q, Jiang H, et al. (2013) Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis. Mol Ther 21, 1345-1357 https://doi.org/10.1038/mt.2013.64
  12. Kim HG, Kwon K, Suh HW, Lee S, Park KH, OY Kwon, et al. (2015) Exosome isolation from hemolymph of Korean rhinoceros beetle, Allomyrina dichotoma (Coleoptera: Scarabaeidae). Entomol Res 45, 339-344. https://doi.org/10.1111/1748-5967.12140
  13. Koles K, Budnik V (2012) Exosomes go with the Wnt. Cell Logistic 2, 169-173. https://doi.org/10.4161/cl.21981
  14. Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y, et al. (2012) Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons. J Biol Chem 287, 16820-16834. https://doi.org/10.1074/jbc.M112.342667
  15. Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen RJ, et al. (2011) Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics 11, 4397-4410. https://doi.org/10.1002/pmic.201000774
  16. Korkut C, Ataman B, Ramachandran P, Ashley J, Barria R, Gherbesi N, et al. (2009) Trans-synaptic transmission of vesicular Wnt signals through Evi/Wntless. Cell 139, 393-404 https://doi.org/10.1016/j.cell.2009.07.051
  17. Kowal J, Tkach M, Thery C (2014) Biogenesis and secretion of exosomes. Curr Opin Cell Biol 29, 116-125. https://doi.org/10.1016/j.ceb.2014.05.004
  18. Lai RC, Yeo RW, Lim SK (2015) Mesenchymal stem cell exosomes. Semin Cell Dev Biol 40, 82-88. https://doi.org/10.1016/j.semcdb.2015.03.001
  19. Mathivanan S, Ji H, Simpson RJ (2010) Exosomes: extracellular organelles important in intercellular communication. J Proteomics 73, 1907-1920. https://doi.org/10.1016/j.jprot.2010.06.006
  20. Micali C, Neumann U, Grunewald D, Panstruga R, O'Connell R (2011) Biogenesis of a specialized plant-fungal interface during host cell internalization of Golovinomyces orontii haustoria. Cell Micorbiol 13, 210-226.
  21. Miranda KC, Bond DT, McKee M, Skog J, Paunescu TG, Da Silva N, et al. (2010) Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease. Kidney Int 78, 191-199. https://doi.org/10.1038/ki.2010.106
  22. Mu J, Zhuang X, Wang Q, Jiang H, Deng ZB, Wang B, et al. (2014) Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles. Mol Nutr Food Res 58, 1561-1573. https://doi.org/10.1002/mnfr.201300729
  23. Nolte EN, Buschow SI, Anderton SM, Stoorvogel W, Wauben MH (2009) Activated T cells recruit exosomes secreted by dendritic cells via LFA-1. Blood 113, 1977-1981. https://doi.org/10.1182/blood-2008-08-174094
  24. Palanisamy V, Sharma S, Deshpande A, Zhou H, Gimzewski J, Wong DT (2010) Nanostructural and transcriptomic analyses of human saliva derived exosomes. Plos One 5, e8577. https://doi.org/10.1371/journal.pone.0008577
  25. Pan BT, Johnstone RM (1983) Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor. Cell 33, 967-978. https://doi.org/10.1016/0092-8674(83)90040-5
  26. Pant S, Hilton H, Burczynski ME (2012) The multifaceted exosome: Biogenesis, role in normal and aberrant cellular function, and frontiers for pharmacological and biomarker opportunities. Biochem Pharmacol 83, 1484-1494. https://doi.org/10.1016/j.bcp.2011.12.037
  27. Piper RC, Katzmann DJ (2007) Biogenesis and function of multivesicular bodies. Annu Rev Cell Dev Biol 23, 519-547. https://doi.org/10.1146/annurev.cellbio.23.090506.123319
  28. Sato-Kuwabara Y, Melo SA, Soares FA, Calin GA (2015) The fusion of two worlds: non-coding RNAs and extracellular vesicles -diagnostic and therapeutic implications. Int J Oncol 46, 17-27. https://doi.org/10.3892/ijo.2014.2712
  29. Simons M, Raposo G (2009) Exosomes- vesicular carriers for intercellular communication. Curr Opin Cell Biol 21, 575-581. https://doi.org/10.1016/j.ceb.2009.03.007
  30. Skogberg G, Lundberg V, Berglund M, Gudmundsdottir J, Telemo E, Lindgren S, et al. (2015) Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens. Immunol Cell Biol 93, 724-734.
  31. Song J, Chen X, Wang M, Xing Y, Zheng Z, Hu S (2014) Cardiac endothelial cell-derived exosomes induce specific regulatory B cells. Sci Rep 4, 7583. https://doi.org/10.1038/srep07583
  32. Subra C, Laulagnier K, Perret B, Record M (2007) Exosome lipidomics unravels lipid sorting at the level of multi-vesicular bodies. Biochimie, 89, 205-212. https://doi.org/10.1016/j.biochi.2006.10.014
  33. Szatanek R, Baran J, Siedlar M, Baj-Krzyworzeka M (2015) Isolation of extracellular vesicles: determining the correct approach. Int J Mol Med 36, 11-17. https://doi.org/10.3892/ijmm.2015.2194
  34. Thery C, Zitvogel L, Amigorena S (2002) Exosomes: composition, biogenesis and function. Nat Rev Immunol 2, 569-579. https://doi.org/10.1038/nri855
  35. Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, et al. (2008) Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science, 319, 1244-1247. https://doi.org/10.1126/science.1153124
  36. Van Der Pol E, Boing AN, Harrison P, Sturk A, Nieuwland R (2012) Classification, functions, and clinical relevance of extracellular vesicles. Pharmacol Rev 64, 676-705. https://doi.org/10.1124/pr.112.005983
  37. Vlassov AV, Magdaleno S, Setterquist R, Conrad R (2012) Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochim Biophys Acta - Gen Subj 1820, 940-948. https://doi.org/10.1016/j.bbagen.2012.03.017
  38. Wang J, Ding Y, Wang J, Hillmer S, Miao Y, Lo SW, et al. (2010) EXPO, an exocyst-positive organelle distinct from multivesicular endosomes and autophagosomes, mediates cytosol to cell wall exocytosis in Arabidopsis and tobacco cells. Plant Cell 22, 4009-4030. https://doi.org/10.1105/tpc.110.080697
  39. Winnebeck EC, Millar CD, Warman GR (2010) Why does insect RNA look degraded? J Insect Sci 10, 159.
  40. Wei T, Hibino H, Omura T (2008) Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells. J Gen Virol 89, 2915-2920. https://doi.org/10.1099/vir.0.2008/002063-0
  41. Wei T, Hibino H, Omura T (2009) Release of Rice dwarf virus from insect vector cells involves secretory exosomes derived from multivesicular bodies. Commun Integr Biol 2, 324-326. https://doi.org/10.4161/cib.2.4.8335
  42. Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, et al. (2003) Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. J Biol Chem 278, 10963-10972. https://doi.org/10.1074/jbc.M207550200
  43. Zhang J, Li S, Li L, Li M, Guo C, Yao J, et al. (2015) Exosome and exosomal microRNA: trafficking, sorting, and function. Genomics Proteomics Bioinformatics 13, 17-24 https://doi.org/10.1016/j.gpb.2015.02.001