Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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STAT3 Potentiates SIAH-1 Mediated Proteasomal Degradation of β-Catenin in Human Embryonic Kidney Cells

  • Shin, Minkyung (Department of Pharmacology, Seoul National University College of Medicine) ;
  • Yi, Eun Hee (Department of Pharmacology, Seoul National University College of Medicine) ;
  • Kim, Byung-Hak (Department of Pharmacology, Seoul National University College of Medicine) ;
  • Shin, Jae-Cheon (Pohang Center for Evaluation of Biomaterials) ;
  • Park, Jung Youl (Industry-Academic Cooperation Foundation, Hanbat National University) ;
  • Cho, Chung-Hyun (Department of Pharmacology, Seoul National University College of Medicine) ;
  • Park, Jong-Wan (Department of Pharmacology, Seoul National University College of Medicine) ;
  • Choi, Kang-Yell (Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University) ;
  • Ye, Sang-Kyu (Department of Pharmacology, Seoul National University College of Medicine)
  • Received : 2016.09.13
  • Accepted : 2016.10.31
  • Published : 2016.11.30
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Abstract

The ${\beta}$-catenin functions as an adhesion molecule and a component of the Wnt signaling pathway. In the absence of the Wnt ligand, ${\beta}$-catenin is constantly phosphorylated, which designates it for degradation by the APC complex. This process is one of the key regulatory mechanisms of ${\beta}$-catenin. The level of ${\beta}$-catenin is also controlled by the E3 ubiquitin protein ligase SIAH-1 via a phosphorylation-independent degradation pathway. Similar to ${\beta}$-catenin, STAT3 is responsible for various cellular processes, such as survival, proliferation, and differentiation. However, little is known about how these molecules work together to regulate diverse cellular processes. In this study, we investigated the regulatory relationship between STAT3 and ${\beta}$-catenin in HEK293T cells. To our knowledge, this is the first study to report that ${\beta}$-catenin-TCF-4 transcriptional activity was suppressed by phosphorylated STAT3; furthermore, STAT3 inactivation abolished this effect and elevated activated ${\beta}$-catenin levels. STAT3 also showed a strong interaction with SIAH-1, a regulator of active ${\beta}$-catenin via degradation, which stabilized SIAH-1 and increased its interaction with ${\beta}$-catenin. These results suggest that activated STAT3 regulates active ${\beta}$-catenin protein levels via stabilization of SIAH-1 and the subsequent ubiquitin-dependent proteasomal degradation of ${\beta}$-catenin in HEK293T cells.

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References

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