DOI QR코드

DOI QR Code

명월초 추출물의 항염 효과에 대한 연구

Anti-inflammation Effect of Gynura Procumbens extract

  • 투고 : 2016.09.01
  • 심사 : 2016.10.20
  • 발행 : 2016.10.28

초록

본 연구는 명월초 추출물의 Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)의 농도 및 Nitric Oxide(NO) 측정을 통하여 현대인들의 피부염증 및 감염질환 예방에 도움이 되는 천연원료 개발을 위하여 수행하였다. 명월초 추출물의 cytosol과 nucleus의 분리과정을 거쳐 Human Dermal Fibroblasts(HDF cell)을 이용하여 다양한 염증조절 인자 중 대표적인 NF-kB 농도를 측정하였고 RAW264.7 세포에 대하여 신경전달 매개체로 피부면역 반응에 중요한 역할을 하는 NO의 양을 분석하였다. 명월초 추출물을 투여한 결과, NF-kB의 단백질과 mRNA 밴드가 감소하여 명월초의 항염 효과가 피부염증 및 질환예방에 효과가 있을 것으로 기대할 수 있었다. 또한 명월초 추출물의 농도가 증가함에 따라 NO는 유의적으로 감소하여 농도 의존적 결과를 보였다. 따라서, 향후 임상적 측면에서의 더 세밀한 항염 효과에 대한 연구가 지속된다면 명월초는 식품으로 뿐 아니라 화장품 등 현대인들이 넓게 적용할 수 있는 융합소재로 이용될 수 있으리라 사료된다.

This study conducted the research about the positive effects of Gynura Procumbens on preventing dermatitis and infectious diseases the contemporary people have. To do it, this study measured the concentration of NF-kB, the extract of Gynura Procumbens. In this regard, cytosol, the extract of Gynura Procumbens, and nucleus went through the separation process. The concentration of major NF-kB among various factors to control inflammation was measured with the use of HDF cell. Regarding RAW264.7 cell, the amounts of NO to play an important role in reacting the skin immune system as the media of neural transmission were analyzed. The outcome about the extracts of Gynura Procumbens injected show that it can be expected that as the NF-kB protein and mRNA band were reduced, Gynura Procumbens would have anti-inflammatory effects that could contribute to preventing dermatitis and diseases. In addition, the extracts of Gynura Procumbens have significantly reduced NO with their concentration increasing. In other words, Gynura Procumbens are considered to regulate dependently the production of NO in the concentration of extracts. Thus there is an expectation that the more intensive research would be conducted to heal dermatitis. And it is deemed that Gynura Procumbens would be used as materials for cosmetics as well as foods that the contemporary people can widely consume if a more careful research about anti-inflammatory effects would be sustained on a human bodies' clinical level.

키워드

참고문헌

  1. J. W. Kim, S. H. Kang, K. W. Lee, "Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells", Journal journal of the Korean Ophthalmological Society, Vol. 54, No. 3, 490-495, 2013. https://doi.org/10.3341/jkos.2013.54.3.490
  2. H. J. Lee, S. E. Oh, "Interrelationship between Environmental Stresses , Reactive Oxygen Species, and Stress - Ethylene", Journal of Ecology and Environment, Vol. 17, No. 1, 91-100, 1994.
  3. Su-Jeong Oh, "Physiological Activating Material Searching and Skin Whitening Improvement Effect of the Extracts from Phragmitis Rhizoma", Doctorate thesis, Graduate School Kwangju women's University. 2011.
  4. S. H. Park, K. O. Cha, "Oriental and Western Food Effects Analysis of Misutgaru for Fusion Remedy in Diabetes Mellitus", Journal of the Korea Convergence Society, Vol. 7 No. 1, pp. 137-143, 2016. https://doi.org/10.15207/JKCS.2016.7.1.137
  5. Eun-Kyung Kim,. "Phytochemical study of Gynura procumbens", Master of Kyoung Hee Univ. 2011.
  6. Hee-Souk Kwon, "Inhibition of melanogenesis and inflammation by ethanol extract of Cordyceps militaris", Ph.D. dissertation, Chungang University. 2005.
  7. J. I. Han, H. H. Sung, C. E. Park, "Study on Convergence Technique Using the Antimicrobial Resistance and Virulence Genes Analysis in Escherichia coli", Journal of the Korea Convergence Society, Vol. 6, No. 5, pp. 77-84, 2015. https://doi.org/10.15207/JKCS.2015.6.5.077
  8. J. H. Ryu, H. Anh, J. Y. Kim, Y. K. Kim, "Inhibitory activity ofplant extracts on nitric oxide synthesis in LPS-activated macrophage", Phytother, Res, Vol. 17 No. 5 pp. 485-489, 2003. https://doi.org/10.1002/ptr.1180
  9. Sur, I. M. Ulvmar., R. Toftgard. "The two-faced NF-kappaB in the skin" Int Rev Immunol, Vol. 27 No. 4 , pp. 205-228, 2008. https://doi.org/10.1080/08830180802130319
  10. C. C. Cha, H.. W. Lee, M. Y. Choi, "Antioxidative and antimicrobial effects of nut species", Kor. J. Pharmacogn, Vol. 112 No. 3 pp.28-34 1998.
  11. Liang. Y. C. Huang. Y. T. Tsai. S. H. Lin-shiau. S. Y. Chen. C. F. Lin. J. K, "Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mousemacrophage", Carcinogenesis, Vol. 20 No. 10 pp. 1945-1952, 1999. https://doi.org/10.1093/carcin/20.10.1945
  12. K. M. Lim., G. T. Jung, D. H. Park, "Study of antimicrobial and DPPH radical scavenger activity of wood vinegar", Korean Journal Biotechnol. Bioeng., Vol. 19 No. 5, pp. 381-384, 2004.
  13. Dahye Park, Eunhee Jang, "Convergence Factors Related to Glycemic Control in Workers with Diabetes Mellitus : using the Korean National Health and Nutrition Examination Survey, 2009-2013", Journal of the Korea Convergence Society, Vol. 6, No. 6, pp. 95-103, 2015. https://doi.org/10.15207/JKCS.2015.6.6.095
  14. Patel. T. N. M. H. Shishehbor, D. L. Bhatt, "A review of high-dose statin therapy: targeting cholesterol and inflammation in atherosclerosis", Eur Heart .Journal, Vol. 28 No. 6, pp. 664-736, 2007. https://doi.org/10.1093/eurheartj/ehl445
  15. Ding. A. H. Nathan. C. F. Stuhr. D. J, "Release of reactive nitrogen intermediates and reactive oxygen intermediates from mousse peritonealmacrophages", J. Immunol, Vol. 141 No 7 .2407-2412, 1988.

피인용 문헌

  1. The Functional Effects on Anti-oxidant and Anti-inflammation of Veronica persica Poir. Extracts vol.26, pp.4, 2018, https://doi.org/10.11625/KJOA.2018.26.4.661