Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena

  • Goo, In Bon (Inland Aquaculture Research Center, National Fisheries Research & Development Institute) ;
  • Im, Jae Hyun (Inland Aquaculture Research Center, National Fisheries Research & Development Institute) ;
  • Gil, Hyun Woo (Dept. of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Lim, Sang Gu (Future Aquaculture Research Center, National Fisheries Research & Development Institute) ;
  • Park, In-Seok (Dept. of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
  • Received : 2015.05.04
  • Accepted : 2015.05.30
  • Published : 2015.09.30
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Abstract

The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

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References

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