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Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro

  • Park, Yun-Gwi (Stem Cell Research Center, Jeju National University) ;
  • Lee, Seung-Eun (Stem Cell Research Center, Jeju National University) ;
  • Kim, Eun-Young (Stem Cell Research Center, Jeju National University) ;
  • Hyun, Hyuk (Stem Cell Research Center, Jeju National University) ;
  • Shin, Min-Young (Stem Cell Research Center, Jeju National University) ;
  • Son, Yeo-Jin (Stem Cell Research Center, Jeju National University) ;
  • Kim, Su-Young (Dept. of Preventive Medicine, College of Medicine, Jeju National University) ;
  • Park, Se-Pill (Stem Cell Research Center, Jeju National University)
  • Received : 2015.05.01
  • Accepted : 2015.05.22
  • Published : 2015.09.30

Abstract

The suitable feeder cell layer is important for culture of embryonic stem (ES) cells. In this study, we investigated the effect of two kinds of the feeder cell, MEF cells and STO cells, layer to mouse ES (mES) cell culture for maintenance of stemness. We compare the colony formations, alkaline phosphatase (AP) activities, expression of pluripotency marker genes and proteins of D3 cell colonies cultured on MEF feeder cell layer (D3/MEF) or STO cell layers (D3/STO) compared to feeder free condition (D3/-) as a control group. Although there were no differences to colony formations and AP activities, interestingly, the transcripts level of pluripotency marker genes, Pou5f1 and Nanog were highly expressed in D3/MEF (79 and 93) than D3/STO (61and 77) or D3/- (65 and 81). Also, pluripotency marker proteins, NANOG and SOX-2, were more synthesized in D3/MEF ($72.8{\pm}7.69$ and $81.2{\pm}3.56$) than D3/STO ($32.0{\pm}4.30$ and $56.0{\pm}4.90$) or D3/- ($55.0{\pm}4.64$ and $62.0{\pm}6.20$). These results suggest that MEF feeder cell layer is more suitable to mES cell culture.

Keywords

References

  1. Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS,Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG (2005) Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122:947-956. https://doi.org/10.1016/j.cell.2005.08.020
  2. Capela A, Temple S (2002) LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal. Neuron 35:865-875. https://doi.org/10.1016/S0896-6273(02)00835-8
  3. Capela A, Temple S (2006) LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1. Developmental Biology 291:300-313. https://doi.org/10.1016/j.ydbio.2005.12.030
  4. Dinsmore J, Ratliff J, Deacon T, Pakzaban P, Jacoby D, Galpern W, Isacson O (1996) Embryonic stem cells differentiated in vitro as a novel source of cells for transplantation. Cell Transplantation 5:131-143. https://doi.org/10.1016/0963-6897(95)02040-3
  5. Gail RM (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proceedings of the National Academy of Sciences of the USA 78:7634-7638. https://doi.org/10.1073/pnas.78.12.7634
  6. Goomez MC, Serrano MA., Earle PC, Jenkins JA, Biancardi MN, Loopez M, Dumas C, Galiguis J, Dresser BL (2010) Derivation of cat embryonic stem-like cells from in vitroproduced blastocysts on homologous and heterologous feeder cells. Theriogenology 74:498-515. https://doi.org/10.1016/j.theriogenology.2010.05.023
  7. Han HW, Kim JH, Kang MJ, Moon SJ, Kang YK, Koo DB, Cho YS (2008) Human embryonic stem cell-derived neuroectodermal spheres revealing neural precursor cell properties. Development & Reproduction 12:87-95.
  8. Julie M, Zhan Z, Wenyu Z, Amy JW, John MH, Claudia MAP, Alexis H, Bradford S, Michael C, Merav B, Muneesh T, Alvin L, Robert V, Robert R, Donald B, Marshall H, Carol W, Anthony B, Michele AC, Jeremy NR, Hannele RB (2011) HIF induces human embryonic stem cell markers in cancer cells. Cancer Cell Research 71:4640-4652. https://doi.org/10.1158/0008-5472.CAN-10-3320
  9. Katerina S, Jilina P, Jilíi P (2015) Alkaline phosphatase in stem cells. Stem Cells International 2015:doi:10.1155/2015/628368
  10. Kenichiro A, Tadahide F, Mamoru N (2006) Conditioned medium from feeder STO cells increases the attachment of mouse embryonic stem cells. Biological and Phamaceutical Bulletin 29:1747-1750. https://doi.org/10.1248/bpb.29.1747
  11. Kim EY, Noh EJ, Park HY, Park MJ, Noh EH, Lee JB, Jeong CJ, Lee DS, Riu KZ, Park SP (2012) Establishment of bovine embryonic stem cell lines using a minimized feeder cell drop. Cellular Reprogramming 14:520-529. https://doi.org/10.1089/cell.2012.0038
  12. Kim SK, Shim JH, Woo DH, Kim JH (2007) Directed differentiation of pancreatic islets from human embryonic stem cells and cell therapy of diabetes mellitus. Development & Reproduction 11:67-77.
  13. Lee SH, Lumelsky N, Studer L, Auerbach JM, McKay RD (2000) Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nature Biotechnology 18:675-679. https://doi.org/10.1038/76536
  14. Levine AJ, Brivanlou AH (2006) GDF3, a BMP inhibitor, regulates cell fate in stem cells and early embryos. Development 133:209-216.
  15. Linzhao C, Holly H, Zhaohui Y, Xiangcan Z, Gautam D (2003) Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells 21:131-142. https://doi.org/10.1634/stemcells.21-2-131
  16. Loh YH, Wu Q, Chew JL, Vega VB, Zhang W, Chen X, Bourque G, George J, Leong B, Liu J (2006) The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nature Genetics 38:431-440. https://doi.org/10.1038/ng1760
  17. Park JH, Kim SJ, Oh EJ, Moon SY, Roh SI, Kim CG, Yoon HS (2003) Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line. Biology of Reproduction 69:2007-2014. https://doi.org/10.1095/biolreprod.103.017467
  18. Park SP, Lee YJ, Lee KS, Shin HA, Cho HY, Chung KS, Kim EY, Lim JH (2004) Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers. Human Reproduction 19:676-684. https://doi.org/10.1093/humrep/deh102
  19. Sachinidis A, Fleischmann BK, Kolossov E, Wartenberg M, Sauer H, Hescheler J (2003) Cardiac specific differrentiation of mouse embryonic stem cells. Cardiovascular Research 58:278-291. https://doi.org/10.1016/S0008-6363(03)00248-7
  20. Schroeder IS, Rolletschek A, Blyszczuk P, Kania G, Wobus AM. (2006) Differentiation of mouse embryonic stem cells to insulin-producing cells. Nature Protocols 1:495-507. https://doi.org/10.1038/nprot.2006.71
  21. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-872. https://doi.org/10.1016/j.cell.2007.11.019
  22. Thomas CD, Harald E, Margot K, Werner S, Rolf K (1985) The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. Journal of Embryology & Experimental Morphology 87:27-45.
  23. Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH (2006) A protein interaction network for pluripotency of embryonic stem cells. Nature 444:364-368. https://doi.org/10.1038/nature05284
  24. Warren LG, Feng Z, Bruce B, Teng M (2007) Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cell. Biochemical and Biophysical Research Communications 358:948-953. https://doi.org/10.1016/j.bbrc.2007.05.054
  25. Ye ZE, Burkholder JK, Qiu P, Schultz JC, Shahidi NT, Yang NS (1994) Establishment of an adherent cell feeder layer from human umbilical cord blood for support of long-term hematopoietic progenitor cell growth. Proceedings of the National Academy of Sciences of the USA 91:12140-12144.
  26. Yue Z, Hongli M, Binata J, Nobuhisa U, Yasushi S, Kenlchi W, Chieko N, Eiki T, Yi W, Yoshihiro I (2015) The significance of membrane fluidity of feeder cellderived substrates for maintenance of iPS cell stemness. Scientific Reports 5:doi:10.1038/srep11386
  27. Zheng B, Mills AA, Bradley A (1999) A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice. Nucleic Acids Research 27:2354-2360. https://doi.org/10.1093/nar/27.11.2354