Journal of Animal Environmental Science (한국축산시설환경학회지)

The Korean Society of Animal Environmental Science & Technology (한국축산환경학회)
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A Bacterial Strain Identified as Bacillus licheniformis using Vitek 2 Effectively Reduced NH3 Emission from Swine Manure

Vitek 2 Compact System을 이용한 Bacillus licheniformis의 동정 및 NH3 저감효과

  • 임정수 (농촌진흥청 국립축산과학원) ;
  • 한덕우 (농촌진흥청 국립축산과학원) ;
  • 이상룡 (농촌진흥청 국립축산과학원) ;
  • 황옥화 (농촌진흥청 국립축산과학원) ;
  • 곽정훈 (농촌진흥청 국립축산과학원) ;
  • 조성백 (농촌진흥청 국립축산과학원)
  • Received : 2015.07.08
  • Accepted : 2015.08.05
  • Published : 2015.09.30
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Abstract

An attempt to produce more pigs in limited spaces inevitably generalized concentrated feeding operation (CFO). As concentrated pig production practice expanded, concerns on environmental issues grow concurrently. Since odor is the concerned most among those, we attempted to develop means to tackle odor emission from livestock operations. Previously, we excavated few microorganisms from pig manure and, one of them, Bacillus licheniformis was particularly useful to handle odor problem. In this study, we conducted our investigation to further characterize Bacillus licheniformis. Strain identification was conducted using Vitek 2 compact, and the optimal temperature and pH conditions to growth B. licheniformis were searched for by analyzing turbidity on O.D 600 nm. Results of this study can be summarized as these, (1) it was re-verified that the bacterial strain that purified from pig manure was, in fact, Bacillus licheniformis, (2) the bacterial growth was highest when the temperature was kept at $30^{\circ}C$, also (3) growth rate was dependent on media pH as it was high at neutral (6, 7 and 8) but dropped when it was diverged from neutral (4, 5, 9 and 10), and (4) regarding ammonia removal efficiency, B. licheniformis recorded 64% effectiveness after 48 h incubation and reached its highest (80%) at 72 h.

Keywords

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