Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Probiotic Mixture KF Attenuates Age-Dependent Memory Deficit and Lipidemia in Fischer 344 Rats

  • Jeong, Jin-Ju (Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Kim, Kyung-Ah (Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Ahn, Young-Tae (R&BD Center, Korea Yakult Co., Ltd.) ;
  • Sim, Jae-Hun (R&BD Center, Korea Yakult Co., Ltd.) ;
  • Woo, Jae-Yeon (Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Huh, Chul-Sung (Graduate School of International Agricultural Technology/GBST, Seoul National University) ;
  • Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Sciences, Kyung Hee University)
  • Received : 2015.05.06
  • Accepted : 2015.05.14
  • Published : 2015.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the memory-enhancing effect of lactic acid bacteria, we selected the probiotic mixture KF, which consisted of Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 (1 × 1011 CFU/g of each strain), and investigated its antilipidemic and memoryenhancing effects in aged Fischer 344 rats. KF (1 × 1010 CFU/rat/day), which was administered orally once a day (6 days per week) for 8 weeks, significantly inhibited age-dependent increases of blood triglyceride and reductions of HDL cholesterol (p < 0.05). KF restored agereduced spontaneous alternation in the Y-maze task to 94.4% of that seen in young rats (p < 0.05). KF treatment slightly, but not significantly, shortened the escape latency daily for 4 days. Oral administration of KF restored age-suppressed doublecortin and brain-derived neurotrophic factor expression in aged rats. Orally administered KF suppressed the expression of p16, p53, and cyclooxygenase-2, the phosphorylation of Akt and mTOR, and the activation of NF-κB in the hippocampus of the brain. These findings suggest that KF may ameliorate age-dependent memory deficit and lipidemia by inhibiting NF-κB activation.

Keywords

References

  1. Bubici C, Papa S, Dean K, Franzoso G. 2006. Mutual crosstalk between reactive oxygen species and nuclear factorkappa B: molecular basis and biological significance. Oncogene 25: 6731-6748. https://doi.org/10.1038/sj.onc.1209936
  2. Cammarota G, Ianiro G, Bibbo S, Gasbarrini A. 2014. Gut microbiota modulation: probiotics, antibiotics or fecal microbiota transplantation? Intern. Emerg. Med. 9: 365-373. https://doi.org/10.1007/s11739-014-1069-4
  3. Chapple IL. 1997. Reactive oxygen species and antioxidants in inflammatory diseases. J. Clin. Periodontol. 24: 287-296 https://doi.org/10.1111/j.1600-051X.1997.tb00760.x
  4. Chung H, Lee E, Choi Y, Kim J, Kim D, Zou Y, et al. 2011. Molecular inflammation as an underlying mechanism of the aging process and age-related diseases. J. Dent. Res. 90: 830-840. https://doi.org/10.1177/0022034510387794
  5. Craft S. 2005. Insulin resistance syndrome and Alzheimer’s disease: age- and obesity-related effects on memory, amyloid, and inflammation. Neurobiol. Aging 26: 65-69. https://doi.org/10.1016/j.neurobiolaging.2005.08.021
  6. Fitzgerald PJ, Seemann JR, Maren S. 2014. Can fear extinction be enhanced? A review of pharmacological and behavioral findings. Brain Res. Bull. 105: 46-60 https://doi.org/10.1016/j.brainresbull.2013.12.007
  7. Hirsch E, Breidert T, Rousselet E, Hunot S, Hartmann A, Michel P. 2003. The role of glial reaction and inflammation in Parkinson’s disease. Ann. NY Acad. Sci. 991: 214-228. https://doi.org/10.1111/j.1749-6632.2003.tb07478.x
  8. Jang SE, Hyam SR, Han MJ, Kim SY, Lee BG, Kim DH. 2013. Lactobacillus brevis G-101 ameliorates colitis in mice by inhibiting NF-κB, MAPK and AKT pathways and by polarizing M1 macrophages to M2-like macrophages. J. Appl. Microbiol. 115: 888-896. https://doi.org/10.1111/jam.12273
  9. Jeong JJ, Kim KA, Jang SE, Woo JY, Han MJ, Kim DH. 2015. Orally administrated Lactobacillus pentosus var. plantarum C29 ameliorates age-dependent colitis by inhibiting the nuclear factor-kappa B signaling pathway via the regulation of lipopolysaccharide production by gut microbiota. PLoS One 10: e9116533. https://doi.org/10.1371/journal.pone.0116533
  10. Luine V, Frankfurt M. 2013. Interactions between estradiol, BDNF and dendritic spines in promoting memory. Neuroscience 239: 34-45. https://doi.org/10.1016/j.neuroscience.2012.10.019
  11. McGeer PL, McGeer EG. 2004. Inflammation and the degenerative diseases of aging. Ann. NY Acad. Sci. 1035: 104-116. https://doi.org/10.1196/annals.1332.007
  12. McKhann GM, Knopman DS, Chertkow H, Hyman BT, Jack Jr CR, Kawas CH, et al. 2011. The diagnosis of dementia due to Alzheimer's disease: recommendations from the National Institute on Aging-Alzheimer's Association workgroups on diagnostic guidelines for Alzheimer's disease. Alzheimer Dement. 7: 263-269. https://doi.org/10.1016/j.jalz.2011.03.005
  13. Michaud M, Balardy L, Moulis G, Gaudin C, Peyrot C, Vellas B, et al. 2013. Proinflammatory cytokines, aging, and age-related diseases. J. Am. Med. Dir. Assoc. 14: 877-882. https://doi.org/10.1016/j.jamda.2013.05.009
  14. Morelli L. 2014. Yogurt, living cultures, and gut health. Am. J. Clin. Nutr. 99: 1248S-1250S. https://doi.org/10.3945/ajcn.113.073072
  15. Neff F, Flores-Dominguez D, Ryan DP, Horsch M, Schroder S, Adler T, et al. 2013. Rapamycin extends murine lifespan but has limited effects on aging. J. Clin. Invest. 123: 3272-3291. https://doi.org/10.1172/JCI67674
  16. Park DY, Ahn YT, Park SH, Huh CS, Yoo SR, Yu R, et al. 2013. Supplementation of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 in diet-induced obese mice is associated with gut microbial changes and reduction in obesity. PLoS One 8: e59470. https://doi.org/10.1371/journal.pone.0059470
  17. Peterson CT, Sharma V, Elmen L, Peterson SN. 2015. Immune homeostasis, dysbiosis and therapeutic modulation of the gut microbiota. Clin. Exp. Immunol. 179: 363-377 https://doi.org/10.1111/cei.12474
  18. Sarter M, Bruno JP. 2004. Developmental origins of the agerelated decline in cortical cholinergic function and associated cognitive abilities. Neurobiol. Aging 25: 1127-1139. https://doi.org/10.1016/j.neurobiolaging.2003.11.011
  19. Terry AV, Buccafusco JJ. 2003. The cholinergic hypothesis of age and Alzheimer’s disease-related cognitive deficits: recent challenges and their implications for novel drug development. J. Pharmacol. Exp. Therap. 306: 821-827. https://doi.org/10.1124/jpet.102.041616
  20. Woo JY, Gu W, Kim KA, Jang SE, Han MJ, Kim DH. 2014. Lactobacillus pentosus var. plantarum C29 ameliorates memory impairment and inflammaging in a D-galactose-induced accelerated aging mouse model. Anaerobe 27: 22-26. https://doi.org/10.1016/j.anaerobe.2014.03.003
  21. Yu BP, Yang R. 1996. Critical evaluation of the free radical theory of aging. Ann. NY Acad. Sci. 786: 1-11. https://doi.org/10.1111/j.1749-6632.1996.tb39047.x

Cited by

  1. The microbiome of the built environment and mental health vol.3, pp.1, 2015, https://doi.org/10.1186/s40168-015-0127-0
  2. Dietary intake of heat-killed Lactococcus lactis H61 delays age-related hearing loss in C57BL/6J mice vol.6, pp.None, 2015, https://doi.org/10.1038/srep23556
  3. Mechanisms and therapeutic effectiveness of lactobacilli vol.69, pp.3, 2015, https://doi.org/10.1136/jclinpath-2015-202976
  4. Effect of Probiotics on Central Nervous System Functions in Animals and Humans: A Systematic Review vol.22, pp.4, 2015, https://doi.org/10.5056/jnm16018
  5. Cafeteria diet and probiotic therapy: cross talk among memory, neuroplasticity, serotonin receptors and gut microbiota in the rat vol.23, pp.2, 2015, https://doi.org/10.1038/mp.2017.38
  6. The Microbiota-Gut-Brain Axis in Neuropsychiatric Disorders: Patho-physiological Mechanisms and Novel Treatments vol.16, pp.5, 2018, https://doi.org/10.2174/1570159x15666170915141036
  7. Effects of Lactobacillus plantarum PS128 on Children with Autism Spectrum Disorder in Taiwan: A Randomized, Double-Blind, Placebo-Controlled Trial vol.11, pp.4, 2015, https://doi.org/10.3390/nu11040820
  8. Probiotic supplementation attenuates hippocampus injury and spatial learning and memory impairments in a cerebral hypoperfusion mouse model vol.46, pp.5, 2015, https://doi.org/10.1007/s11033-019-04949-7
  9. The Microbiota-Gut-Brain Axis vol.99, pp.4, 2015, https://doi.org/10.1152/physrev.00018.2018
  10. Probiotic treatment differentially affects the behavioral and electrophysiological aspects in ethanol exposed animals vol.23, pp.6, 2015, https://doi.org/10.22038/ijbms.2020.41685.9846
  11. The emerging role of probiotics in neurodegenerative diseases: new hope for Parkinson’s disease? vol.16, pp.4, 2021, https://doi.org/10.4103/1673-5374.295270
  12. Modulation of the PI3K/Akt/mTOR signaling pathway by probiotics as a fruitful target for orchestrating the immune response vol.13, pp.1, 2015, https://doi.org/10.1080/19490976.2021.1886844
  13. Interplay of Good Bacteria and Central Nervous System: Cognitive Aspects and Mechanistic Considerations vol.15, pp.None, 2015, https://doi.org/10.3389/fnins.2021.613120
  14. Could the Gut Microbiota Serve as a Therapeutic Target in Ischemic Stroke? vol.2021, pp.None, 2015, https://doi.org/10.1155/2021/1391384
  15. Psychoactive Effects of Lactobacillus johnsonii Against Restraint Stress-Induced Memory Dysfunction in Mice Through Modulating Intestinal Inflammation and permeability-a Study Based on the Gut-Brain A vol.12, pp.None, 2021, https://doi.org/10.3389/fphar.2021.662148