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pxn-1 and pxn-2 May Interact Negatively during Neuronal Development and Aging in C. elegans

  • Cho, Injeong (Department of Biology Education, College of Education, Chosun University) ;
  • Hwang, Gyu Jin (Department of Biology Education, College of Education, Chosun University) ;
  • Cho, Jeong Hoon (Department of Biology Education, College of Education, Chosun University)
  • Received : 2015.05.11
  • Accepted : 2015.06.08
  • Published : 2015.08.31

Abstract

C. elegans has two functional peroxidasins (PXN), PXN-1 and PXN-2. PXN-2 is essential to consolidate the extracellular matrix during development and is suggested to interact with PXN-1 antagonistically. pxn-1 is involved in neuronal development and possibly maintenance; therefore, we investigated the relationship between pxn-1 and pxn-2 in neuronal development and in aging. During neuronal development, defects caused by pxn-1 overexpression were suppressed by overexpression of both pxn-1 and pxn-2. In neuronal aging process, pxn-1 mutants showed less age-related neuronal defects, such as neuronal outgrowth, neuronal wavy processes, and enhanced short-term memory performance. In addition, pxn-2 overexpressing animals retained an intact neuronal morphology when compared with age-matched controls. Consistent with these results, overexpression of both pxn-1 and pxn-2 restored the severe neuronal defects present with pxn-1 overexpression. These results implied that there is a negative relationship between pxn-1 and pxn-2 via pxn-1 regulating pxn-2. Therefore, pxn-1 may function in neuronal development and age-related neuronal maintenance through pxn-2.

Keywords

References

  1. Bargmann, C.I., Hartwieg, E., and Horvitz, H.R. (1993). Odorantselective genes and neurons mediate olfaction in C. elegans. Cell 74, 515-527. https://doi.org/10.1016/0092-8674(93)80053-H
  2. Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71-94.
  3. Busch, S.A., and Silver, J. (2007). The role of extracellular matrix in CNS regeneration. Curr. Opin. Neurobiol. 17, 120-127. https://doi.org/10.1016/j.conb.2006.09.004
  4. Chen, C.H., Chen, Y.C., Jiang, H.C., Chen, C.K., and Pan, C.L. (2013). Neuronal aging: learning from C. elegans. J. Mol. Signal. 8, 14. https://doi.org/10.1186/1750-2187-8-14
  5. Fidler, A.L., Vanacore, R.M., Chetyrkin, S.V., Pedchenko, V.K., Bhave, G., Yin, V.P., Stothers, C.L., Rose, K.L., McDonald, W.H., Clark, T.A., et al. (2014). A unique covalent bond in basement membrane is a primordial innovation for tissue evolution. Proc. Natl. Acad. Sci. USA 111, 331-336. https://doi.org/10.1073/pnas.1318499111
  6. Gotenstein, J.R., Swale, R.E., Fukuda, T., Wu, Z., Giurumescu, C.A., Goncharov, A., Jin, Y., and Chisholm, A.D. (2010). The C. elegans peroxidasin PXN-2 is essential for embryonic morphogenesis and inhibits adult axon regeneration. Development 137, 3603-3613. https://doi.org/10.1242/dev.049189
  7. Jin, Y., Jorgensen, E., Hartwieg, E., and Horvitz, H.R. (1999). The Caenorhabditis elegans gene unc-25 encodes glutamic acid decarboxylase and is required for synaptic transmission but not synaptic development. J. Neurosci. 19, 539-548.
  8. Kamath, R.S., Martinez-Campos, M., Zipperlen, P., Fraser, A.G., and Ahringer, J. (2001). Effectiveness of specific RNAmediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2, RESEARCH0002.
  9. Kauffman, A.L., Ashraf, J.M., Corces-Zimmerman, M.R., Landis, J.N., and Murphy, C.T. (2010). Insulin signaling and dietary restriction differentially influence the decline of learning and memory with age. PLoS Biol. 8, e1000372. https://doi.org/10.1371/journal.pbio.1000372
  10. Kauffman, A., Parsons, L., Stein, G., Wills, A., Kaletsky, R., and Murphy, C. (2011). C. elegans positive butanone learning, shortterm, and long-term associative memory assays. J. Vis. Exp. 11, pii: 2490,
  11. Lee, J., Bandyopadhyay, J., Lee, J.I., Cho, I., Park, D., and Cho, J.H. (2015). A role for peroxidasin PXN-1 in aspects of C. elegans development. Mol. Cells 38, 51-57.
  12. Mello, C., and Fire, A. (1995). DNA transformation. Methods Cell Biol. 48, 451-482. https://doi.org/10.1016/S0091-679X(08)61399-0
  13. Nelson, R.E., Fessler, L.I., Takagi, Y., Blumberg, B., Keene, D.R., Olson, P.F., Parker, C.G., and Fessler, J.H. (1994). Peroxidasin: a novel enzyme-matrix protein of Drosophila development. EMBO J. 13, 3438-3447.
  14. Pan, C.L., Peng, C.Y., Chen, C.H., and McIntire, S. (2011). Genetic analysis of age-dependent defects of the Caenorhabditis elegans touch receptor neurons. Proc. Natl. Acad. Sci. USA 108, 9274-9279. https://doi.org/10.1073/pnas.1011711108
  15. Peterfi, Z., Toth, Z.E., Kovacs, H.A., Lazar, E., Sum, A., Donko, A., Sirokmany, G., Shah, A.M., and Geiszt, M. (2014). Peroxidasinlike protein: a novel peroxidase homologue in the human heart. Cardiovasc Res. 101, 393-399. https://doi.org/10.1093/cvr/cvt256
  16. Soudi, M., Zamocky, M., Jakopitsch, C., Furtmuller, P.G., and Obinger, C. (2012). Molecular evolution, structure, and function of peroxidasins. Chem. Biodivers 9, 1776-1793. https://doi.org/10.1002/cbdv.201100438
  17. Tank, E.M., Rodgers, K.E., and Kenyon, C. (2011). Spontaneous age-related neurite branching in Caenorhabditis elegans. J. Neurosci. 31, 9279-9288. https://doi.org/10.1523/JNEUROSCI.6606-10.2011
  18. Toth, M.L., Melentijevic, I., Shah, L., Bhatia, A., Lu, K., Talwar, A., Naji, H., Ibanez-Ventoso, C., Ghose, P., Jevince, A., et al. (2012). Neurite sprouting and synapse deterioration in the aging Caenorhabditis elegans nervous system. J. Neurosci. 32, 8778-8790. https://doi.org/10.1523/JNEUROSCI.1494-11.2012

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