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Biological Activity and Manufacturing of Yanggeng with Yangha Flower Buds

양하 꽃대의 생리활성 및 양갱 제조

  • Kim, Min-Ju (Department of Alternative Medicine, Kyonggi University) ;
  • Kim, Ae-Jung (Department of Alternative Medicine, Kyonggi University)
  • 김민주 (경기대학교 일반대학원 대체의학과) ;
  • 김애정 (경기대학교 일반대학원 대체의학과)
  • Received : 2015.04.16
  • Accepted : 2015.07.10
  • Published : 2015.08.31

Abstract

This study was performed to investigate the biological activity of yangha flower buds as well as to manufacture of yanggeng prepared with various levels (0 g, 3 g, 6 g, 9 g, and 12 g) of yangha flower buds. DPPH and ABTS scavenging activities of yangha flower buds were 96% and 57% compared to levels of vitamin C, respectively. In the oxygen radical antioxidant capacity assay, antioxidant activity increased dose dependently up to $500{\mu}g/mL$ of yangha flower buds. There was no toxicity up to $1,000{\mu}g/mL$ in vascular smooth muscle cells, and yanggeng significantly reduced migration and proliferation by platelet-derived growth factor-BB-stimulated rat aortic smooth muscle cell migration and proliferation. In the sensory evaluation, the optimal sample was YY9, which was prepared with 9 g of yangha flower buds. It can be concluded that yangha flower buds show antioxidant and vascular protective activities. The optimal sample (YY9) is expected to contribute as a new functional food.

본 연구에서는 폐경 이후 발생률이 높은 심혈관계 질환 예방에 도움을 줄 수 있는 식품을 개발할 목적으로 PDGF-BB로 혈관평활근세포의 이주와 증식을 유도한 다음 양하 꽃대 추출물이 혈관평활근세포의 이주와 증식에 미친 억제 효과를 알아보았다. 그리고 양하 꽃대 시료를 첨가한 양갱을 제조하여 그 품질 특성을 평가, 최적 비율의 양하 꽃대 양갱을 확인하고자 하였다. 혈관평활근세포의 이주에서는 양하 꽃대 추출물 $100{\mu}g/mL$에서부터 정상세포와 유사한 수준을 나타냈다. 또한 증식에서는 양하 꽃대 추출물 $10{\mu}g/mL$에서부터 확연히 억제되어 $1,000{\mu}g/mL$에서는 정상세포 수준까지 떨어졌다. 양하 양갱의 경우 양하 꽃대 시료 첨가량이 높아질수록 명도와 황색도는 유의적(P<0.05)으로 감소한 반면 적색도는 증가하였다. 양하 꽃대를 이용한 양갱의 관능평가 결과를 종합해보면 양하 꽃대 시료 9 g 첨가군(YY9)에서 가장 높은 점수가 나타났다. 양하 꽃대는 항산화 활성 향상과 혈관평활근세포의 이주와 증식을 억제하는 작용이 나타났으므로, 혈관 손상을 예방해줄 수 있는 기능성식품으로서의 가능성을 확인하였다. 따라서 관능평가에서 우수한 점수를 보여준 양하 꽃대 시료 9 g 첨가 양하 양갱은 중년여성의 심혈관질환 예방에 도움을 줄 수 있는 식품으로 기대된다.

Keywords

References

  1. Barrett-Connor E, Grady D. 1998. Hormone replacement therapy, heart disease, and other considerations. Annu Rev Public Health 19: 55-72. https://doi.org/10.1146/annurev.publhealth.19.1.55
  2. Kim CJ, Kim TH, Rhu WS, Ryoo UH. 2000. Influence of menopause on high density lipoprotein-cholesterol and lipids. J Korean Med Sci 15: 380-386. https://doi.org/10.3346/jkms.2000.15.4.380
  3. Yeoum SG. 2003. The investigation on the risk factors of cardiovascular disease for postmenopausal women over 50 years. J Korean Soc Menopause 9: 266-272.
  4. Kang BH, Yoon SJ, Park HM, Huh M. 1999. Effects of therapeutic mode of short-term hormonal replacement therapy by on serum lipids and lipoproteins in hypercholesterolemic postmenopausal women. J Korean Soc Menopause 5: 283-294.
  5. Hattori Y, Matsumura M, Kasai K. 2003. Vascular smooth muscle cell activation by C-reactive protein. Cardiovasc Res 58: 186-195. https://doi.org/10.1016/S0008-6363(02)00855-6
  6. Innes KE, Selfe TK, Taylor AG. 2008. Menopause, the metabolic syndrome, and mind-body therapies. Menopause 15:1005-1013. https://doi.org/10.1097/01.gme.0b013e318166904e
  7. Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL. 1989. Beyond cholesterol. N Engl J Med 320: 915-924. https://doi.org/10.1056/NEJM198904063201407
  8. Braun-Dullaeus RC, Mann MJ, Dzau VJ. 1998. Cell cycle progression: new therapeutic target for vascular proliferative disease. Circulation 98: 82-89. https://doi.org/10.1161/01.CIR.98.1.82
  9. Park KW, Kim JY, Rhyu MR, Choi DS, Bae DS, Yoon BK. 2004. Effects of medicinal plants on proliferation of vascular smooth muscle cells. J Korean Soc Menopause 10:205-209.
  10. Choi SK, Seo YN. 1993. Study on the clonal multiplication of Zingiber mioga ROSC. through in vitro culture of shoot Apex. I. Effects of basal media and growth regulators on plant regeneration and growth of plantlet. Korean J Medicinal Crop Sci 1: 38-42.
  11. Lee JW, Chon SU, Han SK, Choi DG, Ryu J. 2007. Effects of antioxidant and flavor components of Zingiber mioga Rosc. Korean J Medicinal Crop Sci 15: 203-209.
  12. Cho KH, Oh MS, Kim HG, Lee SH, Chung KS, Kim AJ. 2014. Effects of Korean Zingiber mioga R. (flower buds and rhizome) extract on memory. J Korean Soc Food Sci Nutr 43: 1519-1526. https://doi.org/10.3746/jkfn.2014.43.10.1519
  13. Lee WR. 1998. Antioxidant use in coronary artery disease. Korean Circ J 28: 658-662. https://doi.org/10.4070/kcj.1998.28.4.658
  14. Lindenschmidt RC, Tryka AF, Goad ME, Witschi HP. 1986. The effects of dietary butylated hydroxytoluene on liver and colon tumor development in mice. Toxicology 38: 151-160. https://doi.org/10.1016/0300-483X(86)90116-2
  15. Flood MT, Kondo M. 2004. Toxicity evaluation of a ${\beta}$-galactosidase preparation produced by Penicillium multicolor. Regul Toxicol Pharmacol 40: 281-292. https://doi.org/10.1016/j.yrtph.2004.07.011
  16. Abe M, Ozawa Y, Uda Y, Morimitsu Y, Nakamura Y, Osawa T. 2006. A novel labdane-type trialdehyde from myoga (Zingiber miora Roscoe) that potently inhibits human platelet aggregation and human 5-lipoxygenase. Biosci Biotechnol Biochem 70: 2494-2500. https://doi.org/10.1271/bbb.60226
  17. Kim TS. 1998. Natural plant of Korea. Seoul National University Publishing Dep., Seoul, Korea. p 203.
  18. Hirono I, Mori H, Kanto K, Hosaka S, Aiso S. 1982. Carcinogenicity examination of inflorescence of Zingiber mioga Roscoe. Cancer Lett 15: 203-208. https://doi.org/10.1016/0304-3835(82)90119-7
  19. Shin JH, Lee SJ, Sung NJ. 2002. Effects of Zingiber mioga root and Zingiber officinale on the lipid concentration in hyperlipidemic rats. J Korean Soc Food Sci Nutr 31: 679-684. https://doi.org/10.3746/jkfn.2002.31.4.679
  20. Jeong SJ, Im SI, Jung BM. 2005. Comparison of nutritional constituents of native yangha (Zingiber mioga) in Yeosu and Cheju area. Korean J Food Sci Technol 37: 713-716.
  21. Park BH. 2007. Physicochemical properties of jujube paste and quality characteristics of yanggaeng added jujube paste. PhD Dissertation. Sejong University, Seoul, Korea.
  22. KFDA. 2004. Food materials search engine 2004. Available from: http://fse.foodnara.go.kr/origin/search_data_list.jsp (accessed Feb 2015).
  23. Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free-radical method to evaluate antioxidant activity. LWT -Food Sci Technol 28: 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  24. Jeong JH, Jung H, Lee SR, Lee HJ, Hwang KT, Kim TY. 2010. Anti-oxidant, anti-proliferative and anti-inflammatory activities of the extracts from black raspberry fruits and wine. Food Chem 123: 338-344. https://doi.org/10.1016/j.foodchem.2010.04.040
  25. Zheng L, Cao Y, Liu S, Peng Z, Zhang S. 2014. Neferine inhibits angiotensin II-induced rat aortic smooth muscle cell proliferation predominantly by downregulating fractalkine gene expression. Exp Ther Med 8: 1545-1550. https://doi.org/10.3892/etm.2014.1952
  26. Jiang X, Kim B, Lin H, Lee CK, Kim J, Kang H, Lee P, Jung SH, Lee HM, Won KJ. 2010. Tetrahydrobiopterin inhibits PDGF-stimulated migration and proliferation in rat aortic smooth muscle cells via the nitric oxide synthase-independent pathway. Korean J Physiol Pharmacol 14: 177-183. https://doi.org/10.4196/kjpp.2010.14.3.177
  27. Kim MJ. 2013. Quality characteristics of Dolichos lablab Yanggaeng added with Astragalus membranaceus. MS Thesis. Myungji University, Gyeonggi, Korea.
  28. Korotaeva AA, Samoilova EV, Kaminny AI, Pirkova AA, Resink TJ, Erne P, Prokazova NV, Tkachuk VA, Chazov EI. 2005. The catalytically active secretory phospholipase A2 type IIA is involved in restenosis development after PTCA in human coronary arteries and generation of atherogenic LDL. Mol Cell Biochem 270: 107-113. https://doi.org/10.1007/s11010-005-5266-3
  29. Hong Y, Hui SS, Chan BT, Hou J. 2003. Effect of berberine on catecholamine levels in rats with experimental cardiac hypertrophy. Life Sci 72: 2499-2507. https://doi.org/10.1016/S0024-3205(03)00144-9
  30. Mii S, Khalil RA, Morgan KG, Ware JA, Kent KC. 1996. Mitogen-activated protein kinase and proliferation of human vascular smooth muscle cells. Am J Physiol 270: H142-H150.
  31. Tallquist M, Kazlauskas A. 2004. PDGF signaling in cells and mice. Cytokine Growth Factor Rev 15: 205-213. https://doi.org/10.1016/j.cytogfr.2004.03.003
  32. Han JH, Park SN, Yoon MS, Choi OB. 2011. Effects of Houttuynia cordata Thunb extract inhibits on the migration and proliferation of vascular smooth muscle cell. Kor J Pharmacogn 42: 182-186.
  33. Kim AJ. 2012. Quality characteristics of yanggeng prepared with different concentrations of mulberry fruit syrup. J East Asian Soc Dietary Life 22: 62-67.
  34. Oh HL, Ahn MH, Kim NY, Song JE, Lee SY, Song MR, Park JY, Kim MR. 2012. Quality characteristics and antioxidant activities of yanggeng with added Rehmanniae radix Preparata concentrate. Korean J Food Cookery Sci 28:1-8. https://doi.org/10.9724/kfcs.2012.28.1.001

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