Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Optimized pH condition of protein extraction of Gastrodia elata Blume by alkaline method

알칼리에 의한 천마 단백질 추출의 최적 pH 조건

  • 장혜림 (영남대학교 식품영양학과) ;
  • 윤경영 (영남대학교 식품영양학과)
  • Received : 2015.01.02
  • Accepted : 2015.02.12
  • Published : 2015.04.30
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Abstract

This study investigated the optimum pH condition for the efficient extraction of protein from Gastrodia elata Blume. Five extraction pH values (8, 9, 10, 11, and 12) and three precipitation pH values (2, 4, and 6) were used. The protein content, browning degree, and recovery yield of the protein obtained under each pH condition were determined. Most of the G. elata Blume was made up of carbohydrates, and its protein content was also high. The amount of the extracted protein increased according to the increase in the extraction pH, but did not significantly differ between pH 8 and pH 9. The browning degree of the protein significantly increased as the extraction pH increased. The greatest amount of protein was precipitated at pH 4, the recovery yield of which was also the highest. As a result, it was found that the combination of extraction pH 9 and precipitation pH 4, which resulted in a 38.7% recovery yield and a low browning degree, is the optimum condition for the efficient extraction of protein from G. elata Blume.

본 연구에서는 알칼리 사용에 따른 갈변을 최소화하고 영양적으로 우수한 천마 단백질을 보다 효율적으로 추출하기 위하여 용출 및 침전 pH에 따른 단백질의 갈변도와 함량을 측정함으로써 탈지 천마 단백질 알칼리 추출의 최적 pH 조건을 설정하고자 하였다. 천마의 수분은 약 15%로 측정되었으며, 대부분 탄수화물로 구성되어 있었고, 단백질의 함량이 높은 것으로 확인되었다. 이러한 단백질을 다양한 pH에서 용출시킨 결과 pH가 증가함에 따라 용출된 단백질의 양이 증가하였으며, 갈변도 또한 증가함을 보였다. 침전 pH에 따른 단백질 함량은 pH 4에서 침전된 pellet이 가장 많은 함량을 나타내었으며, 상등액의 단백질 함량 또한 대부분 pH 4로 침전시킨 경우 가장 적은 것으로 확인되었다. 뿐만 아니라 단백질의 회수율도 침전 pH가 4일 때 가장 높은 값을 나타내었다. 따라서 갈변도와 단백질 함량을 고려한 탈지 천마 단백질 추출을 위한 최적조건은 용출 pH 9와 침전 pH 4로 결정되었다.

Keywords

References

  1. Shin CS, Park CK, Lee JW, Lee JG, Jang CK, Kim YK (1999) Analysis of the components with freeze drying and steam drying of Gastrodia elata Blume. J Korean Soc Food Sci Nutr, 28, 1058-1063
  2. Choi SR, Jang I, Kim CS, You DH, Kim JY, Kim YG, Ahn YS, Kim JM, Kim YS, Seo KW (2011) Changes of components and quality in Gastrodiae rhizoma by different dry methods. Korean J Medicinal Crop Sci, 19, 354-361 https://doi.org/10.7783/KJMCS.2011.19.5.354
  3. Ha JH, Lee DU, Yong CS, Kim JA, Huh K (1999)Agonistic activities to the benzodiazepine receptor by extracts of medicinal plants (II). Activities of component and active fractions from Gastrodia elata. Kor J Pharmacogn, 30, 284-289
  4. Chung HS, Ji GE (1996) Composition and functionality of Chonma. Korean J Food Sci Technol, 28, 53-57
  5. Lin JH, Liu YC, Hau JP, Wen KC (1996) Parishins B and C from rhizomes of Gastrodia elata. Phytochem, 42, 549-551 https://doi.org/10.1016/0031-9422(95)00955-8
  6. Kim JK, Cha WS, Park JH, Oh SL, Cheon SH, Chung SK (1997) Studies on the mineral component and antioxidative activity of Gastrodia elata Blume. Korean J Post-Harvest Sci Technol Agri Products, 4, 317-321
  7. Lee BY, Choi HS, Hwang JB (2002) Analysis of food components of Gastrodiae Rhizoma and changes in several characteristics at the various drying conditions. Korean J Food Sci Technol, 34, 37-42
  8. Kwon HJ, Lee KH, Kim JH, Chun SS, Cho YJ, Cha WS (2006) Effect of protease on the extraction and properties of the protein from silkworm pupa. J Korean Soc Appl Biol Chem, 49, 304-308
  9. Gnanasambandam R, Heitiarachchy NS (1995) Protein concentrates from unstabilized and stabilized rice bran : preparation and properties. J Food Sci, 60, 1066-1069 https://doi.org/10.1111/j.1365-2621.1995.tb06293.x
  10. Kim W, Jung SY, Hong KW (2011) Optimum pH condition of defatted rice protein extraction by alkaline method. Food Eng Prog, 15, 143-147
  11. Shen L, Wang X, Wang Z, Wu Y, Chen J (2008) Studies on tea protein extraction using alkaline and enzyme methods. Food Chem, 107, 929-938 https://doi.org/10.1016/j.foodchem.2007.08.047
  12. Batista I (1999) Recovery of proteins from fish waste products by alkaline extraction. Eur Food Res Technol, 210, 84-89 https://doi.org/10.1007/s002170050539
  13. Ju ZY, Hettiarachchy NS, Rath N (2001) Extraction, denaturation and hydrophobic properties of rice flour proteins. J Food Sci, 66, 229-232 https://doi.org/10.1111/j.1365-2621.2001.tb11322.x
  14. Song PS, Chichester CO, Stadtman FH (1966) Kinetic behavior and mechanism of inhibition in the Maillard reaction. I. Kinetic behavior of the reaction between D-glucose and glycine. J Food Sci, 31, 906-913 https://doi.org/10.1111/j.1365-2621.1966.tb03269.x
  15. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem, 150, 76-85 https://doi.org/10.1016/0003-2697(85)90442-7
  16. Kim SH, Kim IH, Kang BH, Lee SH, Kim JH, Lee JM (2006) Hot-water extraction condition of Gastrodia elata Blume by response surface methodology. Korean J Food Preserv, 13, 131-137
  17. Kim JM, Moon YS, Yoon KY, Suh SG (2010) Quality properties and preference of fermented Gastrodia elata Blume. Korean J Hort Sci Technol, 28, 507-514
  18. Kang TS, Kong YJ, Kwon HJ, Choi BK, Hong JG, Park YK (2002) A studies on the chemical composition and in vitro biological activities of a hot water extracts of Gastrodia elata. Korean J Mycol, 30, 136-141 https://doi.org/10.4489/KJM.2002.30.2.136
  19. Ajandouz EH, Puigserver A (1999) Nonenzymatic browning reaction of essential amino acids : effect of pH on caramelization and Maillard reaction kinetics. J Agric Food Chem, 47, 1786-1793 https://doi.org/10.1021/jf980928z
  20. Ajandouz EH, Tchiakpe LS, Dalle Ore F, Benajiba A, Puigserver A (2001) Effects of pH on caramelization and Maillard reaction kinetics in fructose-lysine model systems. J Food Sci, 66, 926-931 https://doi.org/10.1111/j.1365-2621.2001.tb08213.x
  21. Kim EJ, Park JR (1995) The effect of protein extraction pH on the components of sesame protein concentrates. J Korean Soc Food Nutr, 24, 613-618
  22. Wang M, Hettiarachchy NS, Qi M, Burks W, Siebenmorgen T (1999) Preparation and functional properties of rice bran protein isolate. J Agric Food Chem, 47, 411-416 https://doi.org/10.1021/jf9806964