Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Application of a Undaria pinnatifida for industrial cultivation of Lactobacillus

유산균의 산업적 배양을 위한 미역의 유용성 평가

  • Chang, Bo Yoon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Han, Ji Hye (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Kim, Ju-Hee (Young Science Scharlau Korea, Jeonnam Biotechnology Research Center) ;
  • Cha, Bum-Suk (Young Science Scharlau Korea, Jeonnam Biotechnology Research Center) ;
  • Ann, Sung-Ho (Young Science Scharlau Korea, Jeonnam Biotechnology Research Center) ;
  • Kim, Sung Yeon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
  • 장보윤 (원광대학교 약학대학 약품연구소) ;
  • 한지혜 (원광대학교 약학대학 약품연구소) ;
  • 김주희 (영사이언스샬라우코리아) ;
  • 차범석 (영사이언스샬라우코리아) ;
  • 안성호 (영사이언스샬라우코리아) ;
  • 김성연 (원광대학교 약학대학 약품연구소)
  • Received : 2015.02.06
  • Accepted : 2015.03.30
  • Published : 2015.04.30
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Abstract

To optimize the medium composition of Undaria pinnatifida as a pH modulator the growth of Lactobacillus plantarum industrial scale, we analyzed the pH and growth characteristics of L. plantarum in basal medium. Subsequently, the medium compositions addition of carbon, nitrogen sources and buffering agents were optimized. When 0.5% yeast extract and 2% glucose, L. plantarum grew to maximum cell density in experimental condition. However, the growth of L. plantarum rapidly pH 4.0 in basal medium. A high alkali-ash value and low cost-effective utilization n the waste part as examined. ddition of U. pinnatifida extract alleviated the serious decrease. Among them, juice of U. pinnatifida was most helpful for the growth of L. plantarum ($36.3{\pm}1.810^8CFU/mL$). These results show that U. pinnatifida be large-scale cultivation of L. plantarum. This optimized U. pinnatifida medium can be used for safe and economical production of Lactobacillus.

산업적 유산균 배양의 가격대비 효율성을 높이기 위하여 질소원, 탄소원 및 무기염물을 기본량으로 기본배지를 제조하고, 효율적인 고농도 유산균체를 수득하기 위하여 pH 조절제로서 따로 첨가되어지는 합성 화학물질을 대신하여 미역을 실험에 사용하였다. 미역의 최적의 첨가 방법을 찾고자 추출, 착즙, 분말의 형태로 배지를 제조하여 유산균증식능 평가한 결과, 착즙의 형태에서 유산균 증식능이 가장 큰 것으로 확인되었다. 이러한 실험 결과를 통해, 폐기되어 환경오염의 원인이 되는 가식부 외 미역을 유산균 배양배지에 착즙형태로 첨가함으로서 종래 pH 조절을 위해 완충액으로 첨가되는 합성 화학물질을 대체하여 활용될 수 있음을 확인하였다. 또한 미역첨가 기본배지 선정에 따른 배지 단가를 절감하면서 높은 균체 수율을 얻는 등 충분한 경제적 장점을 기대할 수 있다. 이러한 연구결과는 미역을 이용함으로써 배양 시 해조류의 식이섬유 등이 정장작용에 유산균의 시너지 효과뿐만 아니라 폐기되는 천연 식물자원을 활용함으로써 새로운 부가가치 창출과 환경오염을 예방하는 효과를 기대할 수 있을 것이다.

Keywords

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