DOI QR코드

DOI QR Code

Indole-3-butyric acid(IBA) 침지처리에 의한 highbush blueberry 'Duke', 'Elizabeth', 'Elliot' (Vaccinium corymbosum L.)의 녹지삽목 발근 촉진

Promoted rooting of softwood cuttings of highbush blueberry 'Duke', 'Elizabeth', 'Elliot' (Vaccinium corymbosum L.) as affected by indole-3-butyric acid(IBA) dipping treatment

  • Kang, S.K. (Korea National College of Agriculture and Fisheries)
  • 발행 : 2015.07.30

초록

간이 녹지삽목시설의 실용성과 발근촉진제 IBA의 발근촉진효과를 조사하기 위하여 북부 highbush blueberry 3품종 'Duke', 'Elizabeth', 'Elliot'를 공시하여 IBA 100ppm, 300ppm, 500ppm, 1,000ppm의 2시간 처리와 3,000ppm 20초 침지처리를 실시한 후 발근특성을 조사하였다. 분산분석에 의한 요인분석 결과, 발근율과 근계지수에 품종효과가 있었으며 발근율, 최대근장, 근계지수에서 IBA의 처리효과도 관찰되었다. 특히 근계지수에서 고도의 유의성 있는 처리효과가 있었다. 그러나 두 요인의 상호작용은 없었다. 무처리구에서 'Duke'의 발근율은 45%, 'Elizabeth'와 'Elliot'는 각각 90%와 85%로 품종간 차이가 있었으며 3품종 모두 고농도 20초 처리와 저농도 2시간 처리구에 의하여 발근이 촉진되었다. 그러나 무처리의 발근율이 낮았던 'Duke'의 발근촉진효과가 다른 두 품종에 비해 현저하였다.

To investigate the practicality of a small scale softwood propagation plastic film tent and the rooting promoting effect of indole-3-butyric acid(IBA) for northern highbush blueberry, softwood cuttings of 3 varieties (Duke, Elizabeth, Elliot) were made after dipping in the IBA solutions of 100ppm, 300ppm, 500ppm, 1,000ppm for 2 hour, and 3,000ppm for 20 seconds, respectively. Two-way ANOVA confirmed the significant differences in the percentage of rooting of cutting, longest root length and rooting volume index (RVI) induced by IBA treatment, as well as the percentage of rooting of cutting and RVI among examined varieties. In particular, the highly significant difference was observed in th RVI for IBA treatment. However, there was no interactions between the auxin treatments and varieties. Spontaneous rooting ability (control) depends on the varieties, showing that the lowest rooting ability was observed in the 'Duke' (45%) followed by 'Elliot'(85%) and 'Elizabeth' (90%). In addition, the rooting of the examined 3 varieties were enhanced by both of short time (20sec) dipping treatment of low level solutions, and long time (2hr) dipping treatment of high level solution, with the remarkably promoted rooting of the 'Duke' cuttings.

키워드

참고문헌

  1. Arai, Y., H. Shidara, and M. Kobayashi. 2004. Effect of top-pinching on hardwood cutting of highbush blueberry varieties. Keisen Jogakuen University Research Bulletin 35:9-11.
  2. Arai, Y. and M. Kobayashi. 2005. Effect of some auxins and bed soil on hardwood cutting of highbush blueberry varieties 'Darrow'. Keisen Jogakuen University Research Bulletin 36:9-11.
  3. Celik H. and M. S. Odabas. 2009. Mathematical modeling of the indole-3- butyric add applications on rooting of northern highbush blueberry (Vaccinium corymbosum L.) softwood-cuttings. Acta Physiologia Plantarum 31: 2915-299.
  4. Debnath, S. C. 2007. Influence of indole-3- butyric add and propagation method on growth and development of in vitro- and ex vitro-derived lowbush blueberry plants. Plant Growth Regulation 51: 245-253.
  5. Douglas, J. 1966. The propagation of highbush blueberries by softwood cuttings. Euphytica 15:304-312.
  6. Eck, P. 1988. Blueberry sdence. 7. advances in propagation, pp. 120-134. Rutgers Univ. Press. New Brunswick, N. J.
  7. Giroux, G. J., B. K. Maynard, and W. A. Johnson. 1999. Comparison of Perlite and Peatperlite Rooting Media for Rooting Softwood Stem Cuttings in a Subirrigation System with Minimal Mist. J. Environ Hort. 17:147-151.
  8. Gonzalez, M. V., M. Lopez, A. E. Valdes, and R. J. Ordas. 2000. Micro-propagation of three berry fruit species using nodal segments from field-grown plants. Annals of Applied Biology 137: 73-78.
  9. Good, G.L. and H.B. Tukey, Jr. 1966. The leaching of metabolites from cuttings propagated under intermittent mist. Proc. Amer. Soc. Hort. Sci. 89:727-733.
  10. Howell, A. B. 2009. Update on health benefits of cranberry and bleuberry. Acta Hort. 810:79-785.
  11. Isutsa D.K., M. P. Pritts, and K. W. Mudge. 1994. Rapid propagation of blueberry plants using ex vitro rooting and controlled acclimatization of micropropagules. HortSdence 29: 1124-1126.
  12. Li S. W., L. Xue, S. Xu, H. Feng, and L. An. 2009. Mediators, genes and signaling in adventitious rooting. Botanical Reviews 75: 230-247.
  13. Litwinczuk W., G. Szczerba, and D. Wrona. 2005. Field performance of highbush blueberries (Vaccinium x corymbosum L.) cv. 'Herbert' propagated by cuttings and tissue culture. Sdentia Horticulturae 06: 162-169.
  14. Ludwig-Muller, J. 2000. Indole-3-butyric add in plant growth and development. Plant Growth Regulation 32: 219-230.
  15. Meiners J., M. Schwab, and I. Szankowski. 2007. Effident in vitro regeneration systems for Vaccinium spedes. Plant Cell Tissue and Organ Culture 676: 169-176.
  16. Mihaljevic, S. and B. Salopek-sondi. 2012. Aanine conjugate of indole-3-butyric add improves rooting of highbush blueberries. Plant Soil Environ 58:236-241.
  17. Moore, J. N. and D. P. Ink. 1964. Effect of rooting medium shading, type of cutting and cold storage of cutting in the propagation of highbush blueberry varieties. Proc. Amer. Soc. Hort. 85:285-294.
  18. Nakazyo, T. 1983. The varietal chatacteristics and cutting propagation of blueberry. Bull. Gunina Hort. Exp. Stn. 11:40-54.
  19. Osterc G., M. Stefancic, and F. Stairpar. 2009. Juvenile stockplant material enhances root development through higher endogenous auxin level. Acta Physiologia Plantarum 31: 899-903.
  20. Pritts, J. N. and J. F. Hancock 1992. Highbush blueberry production guide. Chapter 4, plant selection and propagation. Northeast Regional Agricultural Engineering Service 55:22-23.
  21. Ruzic, D., T. Vujovic, G. Libiakova, R. Gerovic and A. Gajdosova. 2012. Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). J. berry Research 2:97-103.
  22. Stefancic M., F. Stampar, R., Veberic and G. Osterc. 2007. The levels of IAA, IAAsp and some phenolics in cherry rootstock 'Gisela 5' leafy cuttings pretreated with IAA and IBA. Scientia Horticulturae 112: 399-405.
  23. Strik B., G. Fisher, J. Hart, R. Ingham, D. Kaufman, R. Penhallegon, J. Pscheidt, R. William, C. Brun, ML Ahmedullah, A. Antonelli, L. Askham, P. Bristow, D. Havens, B. Scheer, G. Shanks, and D. Barney. 1993. Highbush Blueberry Production Guide. Oregon State University, Comvallis, USA.
  24. Zhang, H. and W.R. Graves. 1995. Subirrigation to root stem cuttings: comparison to intermittent mist and influence of fertilization. HortTechnology 5:265-268.