Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
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Combinatorial Physical Stimulation and Synergistically-Enhanced Fibroblast Differentiation for Skin Regeneration

피부 재생능력 촉진을 위한 물리적 복합자극의 활용 연구

  • Ko, Ung Hyun (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Hong, Jungwoo (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Shin, Hyunjun (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Kim, Cheol Woong (Triple-C Medical Corporation) ;
  • Shin, Jennifer H. (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
  • 고웅현 (한국과학기술원 기계공학과) ;
  • 홍정우 (한국과학기술원 기계공학과) ;
  • 신현준 (한국과학기술원 기계공학과) ;
  • 김철웅 ((주)트리플씨메디칼) ;
  • 신현정 (한국과학기술원 기계공학과)
  • Received : 2015.04.06
  • Accepted : 2015.06.30
  • Published : 2015.08.01
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Abstract

For proper wound healing, dermal contraction and remodeling are critical; during the natural healing process, differentiated fibroblasts called "myofibroblasts" typically undertake these functions. For severe wounds, however, a critical mass of dermal matrix and fibroblasts are lost, making self-regeneration impossible. To overcome this impairment, synthetic wound patches with embedded functional cells can be used to promote healing. In this study, we developed a polydioxanone (PDO)-based cell-embedded sheet on which dermal fibroblasts were cultured and induced for differentiation into myofibroblasts, whereby the following combinatorial physicochemical stimuli were also applied: aligned topology, electric field (EF), and growth factor. The results show that both the aligned topology and EF synergistically enhanced the expression of alpha smooth-muscle actin (${\alpha}$-SMA), a key myofibroblast marker. Our proof-of-concept (POC) experiments demonstrated the potential applicability of a myofibroblast-embedded PDO sheet as a wound patch.

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References

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